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Photoluminesce (PL) spectroscopy offers excellent methods for mapping the PL decay on the nanosecond time scale. However, capturing maps of emission dynamics on the microsecond time scale can be highly time-consuming. We present a new…

光学 · 物理学 2021-02-24 Jiri Junek , Karel Zidek

Intensity correlation microscopy (ICM), which is prominently known through antibunching microscopy or super-resolution optical fluctuation imaging (SOFI), provides superresolution through a correlation analysis of antibunching of…

光学 · 物理学 2018-10-08 Anton Classen , Joachim von Zanthier , Girish S. Agarwal

Measuring subdiffraction separations between single fluorescent particles is important for biological, nano-, and medical-technology studies. Major challenges include (i) measuring changing molecular separations with high temporal…

生物物理 · 物理学 2015-05-18 Shawn H. DeCenzo , Michael C. DeSantis , Y. M. Wang

Bird sound data collected with unattended microphones for automatic surveys, or mobile devices for citizen science, typically contain multiple simultaneously vocalizing birds of different species. However, few works have considered the…

机器学习 · 计算机科学 2013-05-30 Forrest Briggs , Xiaoli Z. Fern , Jed Irvine

Structured illumination microscopy (SIM) has attained high spatiotemporal delineation of subcellular architecture, yet offers limited insight into chemical composition. We develop Chem-SIM, a structured-illumination fluorescence detected…

Subspace methods are essential to high-resolution environment sensing in the emerging unmanned systems, if further combined with the millimeter-wave (mm-Wave) massive multi-input multi-output (MIMO) technique. The estimation of signal/noise…

信号处理 · 电气工程与系统科学 2021-01-13 Li Bin , Wang Shuseng , Zhang Jun , Cao Xianbin , Zhao Chenglin

Imaging across both the full transverse spatial and temporal dimensions of a scene with high precision in all three coordinates is key to applications ranging from LIDAR to fluorescence lifetime imaging. However, compromises that sacrifice,…

图像与视频处理 · 电气工程与系统科学 2021-01-12 C. Callenberg , A. Lyons , D. den Brok , A. Fatima , A. Turpin , V. Zickus , L. Machesky , J. Whitelaw , D. Faccio , M. B. Hullin

Confocal and multiphoton microscopy are effective techniques to obtain high-contrast images of 2-D sections within bulk tissue. However, scattering limits their application to depths only up to ~1 millimeter. Multimode fibers make excellent…

光学 · 物理学 2022-02-23 Sakshi Singh , Simon Labouesse , Rafael Piestun

Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…

生物物理 · 物理学 2023-01-25 Jack W Shepherd , Alex L Payne-Dwyer , Ji-Eun Lee , Aisha Syeda , Mark C Leake

Tissue texture is known to exhibit a heterogeneous or non-stationary nature, therefore using a single resolution approach for optimum classification might not suffice. A clinical decision support system that exploits the subband textural…

计算机视觉与模式识别 · 计算机科学 2015-12-29 Omar S. Al-Kadi

Multicolor super-resolution imaging remains an intractable challenge for both far-field and near-field based super-resolution techniques. Planar super-oscillatory lens (SOL), a far-field subwavelength-focusing diffractive lens device, holds…

Recently, pre-trained models for music information retrieval based on self-supervised learning (SSL) are becoming popular, showing success in various downstream tasks. However, there is limited research on the specific meanings of the…

声音 · 计算机科学 2025-05-23 Yizhi Zhou , Haina Zhu , Hangting Chen

We propose a straightforward sample-based technique to calibrate the axial detection in 3D single molecule localization microscopy (SMLM). Using microspheres coated with fluorescent molecules, the calibration curves of PSF-shaping- or…

Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…

图像与视频处理 · 电气工程与系统科学 2022-01-19 Varun Mannam , Xiaotong Yuan , Scott Howard

Nanosecond temporal resolution enables new methods for wide-field imaging like time-of-flight, gated detection, and fluorescence lifetime. The optical efficiency of existing approaches, however, presents challenges for low-light…

光学 · 物理学 2021-03-09 Adam J. Bowman , Brannon B. Klopfer , Thomas Juffmann , Mark A. Kasevich

Early diagnosis of lung cancer is a key intervention for the treatment of lung cancer computer aided diagnosis (CAD) can play a crucial role. However, most published CAD methods treat lung cancer diagnosis as a lung nodule classification…

图像与视频处理 · 电气工程与系统科学 2022-10-12 Junhua Chen , Haiyan Zeng , Chong Zhang , Zhenwei Shi , Andre Dekker , Leonard Wee , Inigo Bermejo

High-speed image acquisition in light microscopy is essential for a wide range of applications, including observing dynamic biological processes and enabling high-throughput sample analysis. However, traditional imaging speeds are often…

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

光学 · 物理学 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

Fluorescence microscopy is widely employed for the analysis of living biological samples; however, the utility of the resulting recordings is frequently constrained by noise, temporal variability, and inconsistent visualisation of signals…

计算机视觉与模式识别 · 计算机科学 2026-01-16 Hassan Eshkiki , Sarah Costa , Mostafa Mohammadpour , Farinaz Tanhaei , Christopher H. George , Fabio Caraffini

The accurate measurement of complex liquid permittivity in a frequency range provides important information on liquid properties in comparison to single frequency permittivity investigations. The multifrequency microwave characterization…