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Here, we present a novel microscopic technique for measuring wavelength-dependent three-dimensional (3-D) distributions of the refractive indices (RIs) of microscopic samples in the visible wavelengths. Employing 3-D quantitative phase…

光学 · 物理学 2016-04-20 JaeHwang Jung , Kyoohyun Kim , Jonghee Yoon , YongKeun Park

Integrating optical diffraction tomography with imaging flow cytometry enables label-free quantifications of the three-dimensional (3D) morphology and hemoglobin content of red blood cells (RBCs) in their natural form. Self-rotation of RBCs…

医学物理 · 物理学 2023-09-28 Yu-Hsiang Chang , Yang-Hsien Lin , Kung-Bin Sung

Intravital microscopy is an essential tool that reveals behaviours of live cells under conditions close to natural physiological states. So far, although various approaches for imaging cells in vivo have been proposed, most require the use…

生物物理 · 物理学 2016-07-04 Kyoohyun Kim , Kibaek Choe , Inwon Park , Pilhan Kim , YongKeun Park

Label-free single-cell imaging offers a scalable, non-invasive alternative to fluorescence-based cytometry, yet inferring molecular phenotypes directly from bright-field morphology remains challenging. We present a unified Deep Learning…

计算机视觉与模式识别 · 计算机科学 2026-05-15 Saqib Nazir , Ardhendu Behera

Non-invasive morphological assessment is the cornerstone of oocyte and embryo selection in assisted reproductive technology, yet clinical practice remains limited by two-dimensional, qualitative microscopy. While three-dimensional (3D)…

Optical diffraction tomography (ODT) has emerged as a powerful label-free three-dimensional (3D) bioimaging techniques for observing living cells and thin tissue layers. We report a new reflection-mode ODT (rODT) method for imaging thick…

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

光学 · 物理学 2022-01-26 Yi Xue , David Ren , Laura Waller

The examination of blood samples at a microscopic level plays a fundamental role in clinical diagnostics, influencing a wide range of medical conditions. For instance, an in-depth study of White Blood Cells (WBCs), a crucial component of…

计算机视觉与模式识别 · 计算机科学 2023-12-27 Satoshi Tsutsui , Winnie Pang , Bihan Wen

The microscopic examination of white blood cells (WBCs) plays a fundamental role in pathology and is essential for diagnosing blood disorders such as leukemia and anemia. To support further research on WBC images, multiple datasets have…

计算机视觉与模式识别 · 计算机科学 2026-05-20 Satoshi Tsutsui , Winnie Pang , Shuting He , Bihan Wen

The measurement of three-dimensional (3D) images and the analysis of subcellular organelles are crucial for the study of the pathophysiology of cells and tissues. Optical diffraction tomography (ODT) facilitates label-free and quantitative…

图像与视频处理 · 电气工程与系统科学 2019-10-08 Taean Chang , Seungwoo Shin , Moosung Lee , YongKeun Park

Here we report a method for visualization of volumetric structural information of live biological samples with no exogenous contrast agents. The process is made possible through a technique that involves generation, synthesis and analysis…

creating automated processes in different areas of medical science with the application of engineering tools is a highly growing field over recent decades. In this context, many medical image processing and analyzing researchers use…

计算机视觉与模式识别 · 计算机科学 2019-07-17 Hamed Talebi , Amin Ranjbar , Alireza Davoudi , Hamed Gholami , Mohammad Bagher Menhaj

Three-dimensional imaging of biological cells is crucial for the investigation of cell biology, provide valuable information to reveal the mechanisms behind pathophysiology of cells and tissues. Recent advances in optical diffraction…

生物物理 · 物理学 2016-03-03 Kyoohyun Kim , Jonghee Yoon , Seungwoo Shin , SangYun Lee , Su-A Yang , YongKeun Park

Optical diffraction tomography (ODT) is an interferometric microscopy technique capable of measuring 3-D refractive index (RI) distribution of transparent samples. Multiple 2-D holograms of a sample illuminated with various angles are…

White blood cells (WBCs) are the most diverse cell types observed in the healing process of injured skeletal muscles. In the course of healing, WBCs exhibit dynamic cellular response and undergo multiple protein expression changes. The…

图像与视频处理 · 电气工程与系统科学 2024-09-12 Yang Jiao , Hananeh Derakhshan , Barbara St. Pierre Schneider , Emma Regentova , Mei Yang

This paper proposes a novel automatic classification framework for the recognition of five types of white blood cells. Segmenting complete white blood cells from blood smears images and extracting advantageous features from them remain…

计算机视觉与模式识别 · 计算机科学 2020-09-07 Na Dong , Meng-die Zhai , Jian-fang Chang , Chun-ho Wu

Lipid droplets (LDs) are subcellular organelles with important roles in lipid storage and metabolism and involved in various diseases including cancer, obesity, and diabetes. Conventional methods, however, have limited ability to provide…

生物物理 · 物理学 2016-11-08 Kyoohyun Kim , SeoEun Lee , Jonghee Yoon , JiHan Heo , Chulhee Choi , YongKeun Park

Three-dimensional (3D) imaging of the subcellular organisation and morphology of cells and tissues is essential for understanding biological function. Although staining is the most widely used approach for visualising biological samples…

White blood cells, also known as leukocytes are group of heterogeneously nucleated cells which act as salient immune system cells. These are originated in the bone marrow and are found in blood, plasma, and lymph tissues. Leukocytes kill…

图像与视频处理 · 电气工程与系统科学 2023-12-01 Shrijeet Biswas , Amartya Bhattacharya

The automatic detection of White Blood Cells (WBC) still remains as an unsolved issue in medical imaging. The analysis of WBC images has engaged researchers from fields of medicine and computer vision alike. Since WBC can be approximated by…

计算机视觉与模式识别 · 计算机科学 2014-06-30 Erik Cuevas , Margarita Diaz , Miguel Manzanares , Daniel Zaldivar , Marco Perez
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