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Interferometric scattering microscopy (iSCAT) can image the dynamics of nanometer-scale systems. The typical approach to analyzing interferometric images involves intensive processing, which discards data and limits the precision of…

This paper presents an innovative approach to computational acoustic imaging of biperiodic surfaces, exploiting the capabilities of an acoustic superlens to overcome the diffraction limit. We address the challenge of imaging physical…

偏微分方程分析 · 数学 2026-01-05 Yuliang Wang

In this paper, a methodology is investigated for signal recovery in the presence of non-Gaussian noise. In contrast with regularized minimization approaches often adopted in the literature, in our algorithm the regularization parameter is…

最优化与控制 · 数学 2017-01-23 Yosra Marnissi , Yuling Zheng , Emilie Chouzenoux , Jean-Christophe Pesquet

This article presents a method to perform diffraction tomography in a standard microscope that includes an LED array for illumination. After acquiring a sequence of intensity-only images of a thick sample, a ptychography-based…

光学 · 物理学 2015-10-30 Roarke Horstmeyer , Changhuei Yang

Super-resolution microscopy, or nanoscopy, enables the use of fluorescent-based molecular localization tools to study molecular structure at the nanoscale level in the intact cell, bridging the mesoscale gap to classical structural biology…

亚细胞过程 · 定量生物学 2024-05-28 Ivan R. Nabi , Ben Cardoen , Ismail M. Khater , Guang Gao , Timothy H. Wong , Ghassan Hamarneh

We developed a Bessel light sheet fluorescence microscopy (LSFM) system to enable high-speed, wide-field intra-vital imaging of zebrafish and other thick biological samples. This system uses air objectives for the convenient mounting of…

光学 · 物理学 2023-11-07 Chuhui Wang , Jiaju Chen , Cuiyi Peng , Zhenglin Chen , Dongmei Yu , Peiwu Qin

Optical imaging through scattering media is an important challenge in a variety of fields ranging from microscopy to autonomous vehicles. While advanced wavefront shaping techniques have offered significant breakthroughs in the past decade,…

光学 · 物理学 2023-05-23 Omri Haim , Jeremy Boger-Lombard , Ori Katz

We report an angle-tilted, wavelength-multiplexed ptychographic modulation approach for multispectral lensless on-chip microscopy. In this approach, we illuminate the specimen with lights at 5 wavelengths simultaneously. A prism is added at…

Fisheye lens, which is suitable for panoramic imaging, has the prominent advantage of a large field of view and low cost. However, the fisheye image has a severe geometric distortion which may interfere with the stage of image registration…

计算机视觉与模式识别 · 计算机科学 2023-07-25 Jing Hao , Jingming Xie , Jinyuan Zhang , Moyun Liu

A solution to the inversion problem of scattering would offer aberration-free diffraction-limited 3D images without the resolution and depth-of-field limitations of lens-based tomographic systems. Powerful algorithms are increasingly being…

Diffraction-limited imaging in epi-fluorescence microscopy remains a challenge when sample aberrations are present or when the region of interest rests deep within an inhomogeneous medium. Adaptive optics is an attractive solution albeit…

Fluorescence microscopy is rapidly turning into nanoscopy. Among the various nanoscopy methods, the STED/RESOLFT super-resolution family has recently been expanded to image even large fields of view within a few seconds. This advance relies…

Lensless in-line holography is a simple, portable, and cost-effective method of imaging especially for the biomedical microscopy applications. We propose a multiplicative gradient descent optimization based method to obtain multi-depth…

图像与视频处理 · 电气工程与系统科学 2020-04-20 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

Ptychography is a lensless imaging method that allows for wavefront sensing and phase-sensitive microscopy from a set of diffraction patterns. Recently, it has been shown that the optimization task in ptychography can be achieved via…

图像与视频处理 · 电气工程与系统科学 2021-01-13 Jacob Seifert , Dorian Bouchet , Lars Loetgering , Allard P. Mosk

The single sideband (SSB) framework of analytical electron ptychography can account for the presence of residual geometrical aberrations induced by the probe-forming lens. However, the accuracy of this aberration correction method is highly…

Lensless imaging is a popular research field for the advantages of small size, wide field-of-view and low aberration in recent years. However, some traditional lensless imaging methods suffer from slow convergence, mechanical errors and…

The action of a nanoscopic spherically symmetric refractive index profile on a focused Gaussian beam may easily be envisaged as the action of a phase-modifying element, i.e. a lens: Rays traversing the inhomogeneous refractive index field…

光学 · 物理学 2011-09-14 Selmke Markus , Braun Marco , Cichos Frank

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

Lensless cameras offer significant advantages in size, weight, and cost compared to traditional lens-based systems. Without a focusing lens, lensless cameras rely on computational algorithms to recover the scenes from multiplexed…

图像与视频处理 · 电气工程与系统科学 2024-10-08 Xin Cai , Zhiyuan You , Hailong Zhang , Wentao Liu , Jinwei Gu , Tianfan Xue

Wavefront shaping is increasingly being used in modern microscopy to obtain distortion-free, high-resolution images deep inside inhomogeneous media. Wavefront shaping methods typically rely on the presence of a 'guidestar' in order to find…

光学 · 物理学 2023-07-19 Abhilash Thendiyammal , Gerwin Osnabrugge , Tom Knop , Ivo M. Vellekoop