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The scanning electron microscopy (SEM) is probably one the most fascinating examination approach that has been used since more than two decades to detailed inspection of micro scale objects. Most of the scanning electron microscopes could…

计算机视觉与模式识别 · 计算机科学 2016-02-18 Wichai Shanklin

Deep learning techniques have shown their success in medical image segmentation since they are easy to manipulate and robust to various types of datasets. The commonly used loss functions in the deep segmentation task are pixel-wise loss…

图像与视频处理 · 电气工程与系统科学 2022-10-10 Yuan Lan , Yang Xiang , Luchan Zhang

Medical image segmentation has become an essential technique in clinical and research-oriented applications. Because manual segmentation methods are tedious, and fully automatic segmentation lacks the flexibility of human intervention or…

图像与视频处理 · 电气工程与系统科学 2019-04-24 Kevin Karsch , Qing He , Ye Duan

Deep image prior (DIP) has recently attracted attention owing to its unsupervised positron emission tomography (PET) image reconstruction, which does not require any prior training dataset. In this paper, we present the first attempt to…

医学物理 · 物理学 2023-08-08 Fumio Hashimoto , Yuya Onishi , Kibo Ote , Hideaki Tashima , Taiga Yamaya

Differentiating signals from the background in micrographs is a critical initial step for cryogenic electron microscopy (cryo-EM), yet it remains laborious due to low signal-to-noise ratio (SNR), the presence of contaminants and densely…

图像与视频处理 · 电气工程与系统科学 2025-09-30 Szu-Chi Chung , Po-Cheng Chou

In photon upconverting core-shell nanoparticles, structure strongly dictates performance. Conventional imaging in scanning transmission electron microscopy has sufficient resolution to probe the atomic structure of these nanoparticles, but…

We present a numerically efficient method to reconstruct a disordered network of thin biopolymers, such as collagen gels, from three-dimensional (3D) image stacks recorded with a confocal microscope. Our method is based on a template…

定量方法 · 定量生物学 2011-11-17 Patrick Krauss , Claus Metzner , Janina Lange , Nadine Lang , Ben Fabry

Temporal focusing two-photon microscopy enables high resolution imaging of fine structures in vivo over a large volume. A limitation of temporal focusing is that signal-to-background ratio and resolution degrade rapidly with increasing…

Convolutional autoencoders have emerged as popular methods for unsupervised defect segmentation on image data. Most commonly, this task is performed by thresholding a pixel-wise reconstruction error based on an $\ell^p$ distance. This…

计算机视觉与模式识别 · 计算机科学 2019-04-09 Paul Bergmann , Sindy Löwe , Michael Fauser , David Sattlegger , Carsten Steger

The concept of compressive sensing was recently proposed to significantly reduce the electron dose in scanning transmission electron microscopy (STEM) while still maintaining the main features in the image. Here, an experimental setup based…

仪器与探测器 · 物理学 2016-03-23 Armand Béché , Bart Goris , Bert Freitag , Jo Verbeeck

Lensless in-line holography is a simple, portable, and cost-effective method of imaging especially for the biomedical microscopy applications. We propose a multiplicative gradient descent optimization based method to obtain multi-depth…

图像与视频处理 · 电气工程与系统科学 2020-04-20 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

Neurosurgery interventions involve complex tracking systems because a tissue deformation takesplace. The neuronavigation system relies only on preoperative images. In order to overcome the soft tissue deformations and guarantee the accuracy…

医学物理 · 物理学 2007-10-03 Claudio Lobos , Marek Bucki , Yohan Payan , Nancy Hitschfeld

Expansion Microscopy is a super-resolution technique in which physically enlarging samples in an isotropic manner increases inter-molecular distances such that nano-scale structures can be resolved using light microscopy. This is…

神经元与认知 · 定量生物学 2025-01-22 Shakila Behzadi , Jacquelin Ho , Zainab Tanvir , Gal Haspel , Limor Freifeld , Kristen E. Severi

The main focus of this work is a novel framework for the joint reconstruction and segmentation of parallel MRI (PMRI) brain data. We introduce an image domain deep network for calibrationless recovery of undersampled PMRI data. The proposed…

图像与视频处理 · 电气工程与系统科学 2021-02-03 Aniket Pramanik , Mathews Jacob

Neuropathological analyses benefit from spatially precise volumetric reconstructions that enhance anatomical delineation and improve morphometric accuracy. Our prior work has shown the feasibility of reconstructing 3D brain volumes from 2D…

Compressed sensing can decrease scanning transmission electron microscopy electron dose and scan time with minimal information loss. Traditionally, sparse scans used in compressed sensing sample a static set of probing locations. However,…

机器学习 · 计算机科学 2021-03-12 Jeffrey M. Ede

Analytical electron microscopy and spectroscopy of biological specimens, polymers, and other beam sensitive materials has been a challenging area due to irradiation damage. There is a pressing need to develop novel imaging and spectroscopic…

The automated segmentation and tracking of macrophages during their migration are challenging tasks due to their dynamically changing shapes and motions. This paper proposes a new algorithm to achieve automatic cell tracking in time-lapse…

图像与视频处理 · 电气工程与系统科学 2023-01-03 Seol Ah Park , Tamara Sipka , Zuzana Kriva , George Lutfalla , Mai Nguyen-Chi , Karol Mikula

We demonstrate a multi-beam scanning transmission electron microscopy (STEM) imaging that integrates down-sampling with super-resolution image reconstruction via a compressive sensing framework. A custom condenser aperture with six randomly…

仪器与探测器 · 物理学 2026-03-19 Akira Yasuhara , Takumi Sannomiya , Ryoichi Horisaki

Automatic 3D neuron reconstruction is critical for analysing the morphology and functionality of neurons in brain circuit activities. However, the performance of existing tracing algorithms is hinged by the low image quality. Recently, a…

图像与视频处理 · 电气工程与系统科学 2021-09-17 Heng Wang , Chaoyi Zhang , Jianhui Yu , Yang Song , Siqi Liu , Wojciech Chrzanowski , Weidong Cai