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We introduce and parameterize a chemomechanical model of microtubule dynamics on the dimer level, which is based on the allosteric tubulin model and includes attachment, detachment and hydrolysis of tubulin dimers as well as stretching of…

亚细胞过程 · 定量生物学 2021-08-31 Matthias Schmidt , Jan Kierfeld

Certain regulatory proteins influence the polymerization dynamics of microtubules by inducing catastrophe with a rate that depends on the microtubule length. Using a discrete formulation, here we show that, for a catastrophe rate…

生物物理 · 物理学 2012-08-27 Vandana Yadav , Sutapa Mukherji

Microtubule dynamic instability arises from the hydrolysis of GTP bound to the beta-monomer of the tubulin dimer. The conformational change induced by hydrolysis is unknown, but microtubules disassemble into protofilaments of GDP-bound…

生物物理 · 物理学 2007-05-23 Deborah Kuchnir Fygenson

We investigate the microtubule polymerization dynamics with catastrophe and rescue events for three different confinement scenarios, which mimic typical cellular environments: (i) The microtubule is confined by rigid and fixed walls, (ii)…

亚细胞过程 · 定量生物学 2015-05-12 Björn Zelinski , Nina Müller , Jan Kierfeld

Microtubules are stiff filamentary proteins that constitute an important component of the cytoskeleton of cells. These are known to exhibit a dynamic instability. A steadily growing microtubule can suddenly start depolymerizing very…

统计力学 · 物理学 2009-11-10 Pankaj Kumar Mishra , Ambarish Kunwar , Sutapa Mukherji , Debashish Chowdhury

Microtubules are a major component of the cytoskeleton distinguished by highly dynamic behavior both in vitro and in vivo. We propose a general mathematical model that accounts for the growth, catastrophe, rescue and nucleation processes in…

生物物理 · 物理学 2015-05-13 Peter Hinow , Vahid Rezania , Jack A. Tuszynski

Microtubules, the primary components of the chromosome segregation machinery, are stabilized by longitudinal and lateral non-covalent bonds between the tubulin subunits. However, the thermodynamics of these bonds and the microtubule…

We investigate the cooperative dynamics of an ensemble of N microtubules growing against an elastic barrier. Microtubules undergo so-called catastrophes, which are abrupt stochastic transitions from a growing to a shrinking state, and…

亚细胞过程 · 定量生物学 2013-01-08 Björn Zelinski , Jan Kierfeld

A simple stochastic model which describes microtubule dynamics and explicitly takes into account the relevant biochemical processes is presented. The model incorporates binding and unbinding of monomers and random phosphate release inside…

统计力学 · 物理学 2015-05-27 Ranjith Padinhateeri , Anatoly B. Kolomeisky , David Lacoste

Dynamic instability of microtubules is considered using frameworks of non-linear thermodynamics and non-equilibrium reaction-diffusion systems. Stochastic assembly/disassembly phases in the polymerization dynamics of microtubules are…

亚细胞过程 · 定量生物学 2016-11-15 Eugene Katrukha

A novel theoretical model of dynamic instability of a system of linear (1D) microtubules (MTs) in a bounded domain is introduced for studying the role of a cell edge in vivo and analyzing the effect of competition for a limited amount of…

亚细胞过程 · 定量生物学 2007-05-23 Gennady Margolin , Ivan V. Gregoretti , Holly V. Goodson , Mark S. Alber

In the mitotic spindle microtubules attach to kinetochores via catch bonds during metaphase, and microtubule depolymerization forces give rise to stochastic chromosome oscillations. We investigate the cooperative stochastic microtubule…

亚细胞过程 · 定量生物学 2020-03-17 Felix Schwietert , Jan Kierfeld

The microtubule assembly process has been extensively studied, but the underlying molecular mechanism remains poorly understood. The structure of an artificially generated sheet polymer that alternates two types of lateral contacts and that…

生物大分子 · 定量生物学 2009-11-10 Zhanghan Wu , Hong-Wei Wang , Weihua Mu , Zhongcan Ouyang , Eva Nogales , Jianhua Xing

We describe simulations of a microscopic elastic filament immersed in a fluid and subject to a uniform external force. Our method accounts for the hydrodynamic coupling between the flow generated by the filament and the friction force it…

软凝聚态物质 · 物理学 2009-11-11 M. Cosentino Lagomarsino , I. Pagonabarraga , C. P. Lowe

Microtubules (MTs) are cytoplasmic protein polymers that are essential for fundamental cellular processes including the maintenance of cell shape, organelle transport and formation of the mitotic spindle. Microtubule dynamic instability is…

生物大分子 · 定量生物学 2014-04-09 Chunlei Li , Jun Li , Holly V. Goodson , Mark S. Alber

In this paper a quantum mechanical description of the assembly/disassembly process for microtubules is proposed. We introduce creation and annihilation operators that raise or lower the microtubule length by a tubulin layer. Following that,…

生物大分子 · 定量生物学 2008-10-22 Vahid Rezania , Jack Tuszynski

The structural integrity of microtubules is paramount for cellular function. We present a theoretical analysis of their lattice fracture, focusing on the influence of multi-seam structures arising from monomer defects and aiming to provide…

生物物理 · 物理学 2025-11-11 Amir Zablotsky , Subham Biswas , Laura Schaedel , Karin John

We investigate stall force and polymerization kinetics of rigid protofilaments in a microtubule or interacting filaments in bundles under an external load force in the framework of a discrete growth model. We introduce the concecpt of…

统计力学 · 物理学 2011-02-11 Jaroslaw Krawczyk , Jan Kierfeld

In this paper a quantum mechanical description of the assembly/disassembly process for microtubules is proposed. We introduce creation and annihilation operators that raise or lower the microtubule length by a tubulin layer. Following that,…

细胞行为 · 定量生物学 2007-05-23 Vahid Rezania , Jack Tuszynski

Buckling induced by viscous flow changes the shape of sheet-like nanomaterial particles suspended in liquids. This instability at the particle scale affects collective behavior of suspension flows and has many technological and biological…

流体动力学 · 物理学 2023-03-10 Hugo Perrin , Heng Li , Lorenzo Botto
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