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Being a dual purpose enzyme, the DNA polymerase is responsible for elongation of the newly formed DNA strand as well as cleaving the erroneous growth in case of a misincorporation. The efficiency of replication depends on the coordination…

软凝聚态物质 · 物理学 2021-10-13 M Sahoo , Arsha N , P R Baral , S Klumpp

DNA polymerase (DNAP) is a dual-purpose enzyme that plays two opposite roles in two different situations during DNA replication. It plays its normal role as a {\it polymerase} catalyzing the elongation of a new DNA molecule by adding a…

生物物理 · 物理学 2013-08-13 Ajeet K. Sharma , Debashish Chowdhury

A chemical kinetic model of the elongation dynamics of RNA polymerase along a DNA sequence is introduced. The proposed model governs the discrete movement of the RNA polymerase along a DNA template, with no consideration given to elastic…

生物大分子 · 定量生物学 2007-05-23 Yujiro Richard Yamada , Charles S. Peskin

In biological cells, DNA replication is carried out by the replisome, a protein complex encompassing multiple DNA polymerases. DNA replication is semi-discontinuous: a DNA polymerase synthesizes one (leading) strand of the DNA continuously,…

生物物理 · 物理学 2025-11-11 Janani G , Deepak Bhat

DNA polymerases are an essential class of enzymes or molecular motors that catalyze processive DNA syntheses during DNA replications. A critical issue for DNA polymerases is their molecular mechanism of processive DNA replication. We have…

软凝聚态物质 · 物理学 2021-11-01 Yu-Ru Liu , Peng-Ye Wang , Wei Li , Ping Xie

A DNA polymerase (DNAP) replicates a template DNA strand. It also exploits the template as the track for its own motor-like mechanical movement. In the polymerase mode it elongates the nascent DNA by one nucleotide in each step. But,…

亚细胞过程 · 定量生物学 2013-10-16 Ajeet K. Sharma , Debashish Chowdhury

Conventionally in a PCR reaction, Primers binds to DNA template in an antiparallel manner and template DNA is amplified as it is. Here we describe an approach in which First primer binds in a complementary parallel orientation leading to…

基因组学 · 定量生物学 2013-11-07 Bhardwaj Vikash , Sharma Kulbhushan

Polymerases are protein enzymes that move along nucleic acid chains and catalyze template-based polymerization reactions during gene transcription and replication. The polymerases also substantially improve transcription or replication…

生物物理 · 物理学 2017-01-19 Jin Yu

DNA codes have many applications, such as in data storage, DNA computing, etc. Good DNA codes have large sizes and satisfy some certain constraints. In this paper, we present a new construction method for reversible DNA codes. We show that…

信息论 · 计算机科学 2024-03-19 Xueyan Chen , Whan-Hyuk Choi , Hongwei Liu

Polymerization of RNA from a template DNA is carried out by a molecular machine called RNA polymerase (RNAP). It also uses the template as a track on which it moves as a motor utilizing chemical energy input. The time it spends at each…

统计力学 · 物理学 2015-05-13 Tripti Tripathi , Gunter M. Schütz , Debashish Chowdhury

A class of nucleosome remodeling motors translocate nucleosomes, to which they are attached, toward the middle of DNA chain in the presence of ATP during in vitro experiments. Such a biological activity is likely based on a physical…

生物物理 · 物理学 2016-05-23 Kuni H. Iwasa , Ana Maria Florescu

Transcription is a key process in gene expression, in which RNA polymerases produce a complementary RNA copy from a DNA template. RNA polymerization is frequently interrupted by backtracking, a process in which polymerases perform a random…

生物物理 · 物理学 2016-06-29 Édgar Roldán , Ana Lisica , Daniel Sánchez-Taltavull , Stephan W. Grill

The effect of sequence heterogeneity on polynucleotide translocation across a pore and on simple models of molecular motors such as helicases, DNA polymerase/exonuclease and RNA polymerase is studied in detail. Pore translocation of RNA or…

统计力学 · 物理学 2009-11-10 Yariv Kafri , David K. Lubensky , David R. Nelson

We propose a new method for electrophoretic separation of DNA in which adsorbed polymers are driven over a disordered two-dimensional substrate which contains attractive sites for the polymers. Using simulations of a model for long polymer…

软凝聚态物质 · 物理学 2008-03-31 C. J. Olson Reichhardt , C. Reichhardt

A multiscale approach is used to simulate the translocation of DNA through a nanopore. Within this scheme, the interactions of the molecule with the surrounding fluid (solvent) are explicitly taken into account. By generating polymers of…

生物物理 · 物理学 2009-11-13 Simone Melchionna , Maria Fyta , Efthimios Kaxiras , Sauro Succi

The two strands of a DNA molecule with a repetitive sequence can pair into many different basepairing patterns. For perfectly periodic sequences, early bulk experiments of Poerschke indicate the existence of a sliding process, permitting…

生物大分子 · 定量生物学 2007-05-23 Richard A. Neher , Ulrich Gerland

DNA renaturation is the recombination of two complementary single strands to form a double helix. It is experimentally known that renaturation proceeds through the formation of a double stranded nucleus of several base pairs (the rate…

软凝聚态物质 · 物理学 2010-04-12 A. Ferrantini , M. Baiesi , E. Carlon

Charge inversion of a DNA double helix by an oppositely charged flexible polyelectrolyte (PE) is considered. We assume that, in the neutral state of the DNA-PE complex, each of the DNA charges is locally compensated by a PE charge. When an…

软凝聚态物质 · 物理学 2009-11-07 T. T. Nguyen , B. I. Shklovskii

Charge inversion of a DNA double helix by an oppositely charged flexible polyelectrolyte (PE) is widely used for gene delivery. It is considered here in terms of discrete charges of DNA. We concentrate on the worst scenario case when in the…

软凝聚态物质 · 物理学 2009-11-07 T. T. Nguyen , B. I. Shklovskii

DNA nanostructures are made using synthetic DNA strands, the sequences of which are designed such that they will self-assemble into the desired form by hybridization of complementary domains. Various structures and devices have been…

生物物理 · 物理学 2015-06-02 Katherine E. Dunn
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