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The prospect of neural reconstruction from Electron Microscopy (EM) images has been elucidated by the automatic segmentation algorithms. Although segmentation algorithms eliminate the necessity of tracing the neurons by hand, significant…

计算机视觉与模式识别 · 计算机科学 2015-09-29 Toufiq Parag

Automated sample preparation and electron microscopy enables acquisition of very large image data sets. These technical advances are of special importance to the field of neuroanatomy, as 3D reconstructions of neuronal processes at the nm…

A central problem in neuroscience is reconstructing neuronal circuits on the synapse level. Due to a wide range of scales in brain architecture such reconstruction requires imaging that is both high-resolution and high-throughput. Existing…

计算机视觉与模式识别 · 计算机科学 2012-10-03 Tao Hu , Juan Nunez-Iglesias , Shiv Vitaladevuni , Lou Scheffer , Shan Xu , Mehdi Bolorizadeh , Harald Hess , Richard Fetter , Dmitri Chklovskii

The alignment of serial-section electron microscopy (ssEM) images is critical for efforts in neuroscience that seek to reconstruct neuronal circuits. However, each ssEM plane contains densely packed structures that vary from one section to…

计算机视觉与模式识别 · 计算机科学 2017-12-06 Inwan Yoo , David G. C. Hildebrand , Willie F. Tobin , Wei-Chung Allen Lee , Won-Ki Jeong

Neural circuits can be reconstructed from brain images acquired by serial section electron microscopy. Image analysis has been performed by manual labor for half a century, and efforts at automation date back almost as far. Convolutional…

计算机视觉与模式识别 · 计算机科学 2019-05-01 Kisuk Lee , Nicholas Turner , Thomas Macrina , Jingpeng Wu , Ran Lu , H. Sebastian Seung

Reconstruction of neuroanatomy is a fundamental problem in neuroscience. Stochastic expression of colors in individual cells is a promising tool, although its use in the nervous system has been limited due to various sources of variability…

神经元与认知 · 定量生物学 2017-01-24 Uygar Sümbül , Douglas Roussien , Fei Chen , Nicholas Barry , Edward S. Boyden , Dawen Cai , John P. Cunningham , Liam Paninski

Automated and semi-automated techniques in biomedical electron microscopy (EM) enable the acquisition of large datasets at a high rate. Segmentation methods are therefore essential to analyze and interpret these large volumes of data, which…

计算机视觉与模式识别 · 计算机科学 2023-08-08 Anusha Aswath , Ahmad Alsahaf , Ben N. G. Giepmans , George Azzopardi

Comprehensive, synapse-resolution imaging of the brain will be crucial for understanding neuronal computations and function. In connectomics, this has been the sole purview of volume electron microscopy (EM), which entails an excruciatingly…

图像与视频处理 · 电气工程与系统科学 2023-03-03 Yicong Li , Yaron Meirovitch , Aaron T. Kuan , Jasper S. Phelps , Alexandra Pacureanu , Wei-Chung Allen Lee , Nir Shavit , Lu Mi

To build the connectomics map of the brain, we developed a new algorithm that can automatically refine the Membrane Detection Probability Maps (MDPM) generated to perform automatic segmentation of electron microscopy (EM) images. To achieve…

神经与进化计算 · 计算机科学 2015-06-22 Xundong Wu

This paper addresses the problem of reassembling images from disjointed fragments. More specifically, given an unordered set of fragments, we aim at reassembling one or several possibly incomplete images. The main contributions of this work…

计算机视觉与模式识别 · 计算机科学 2018-09-05 M. -M. Paumard , D. Picard , H. Tabia

Reconstructing a map of neuronal connectivity is a critical challenge in contemporary neuroscience. Recent advances in high-throughput serial section electron microscopy (EM) have produced massive 3D image volumes of nanoscale brain tissue…

The emerging field of connectomics aims to unlock the mysteries of the brain by understanding the connectivity between neurons. To map this connectivity, we acquire thousands of electron microscopy (EM) images with nanometer-scale…

定量方法 · 定量生物学 2016-04-04 Stephen M. Plaza , Stuart E. Berg

This paper presents a new regularization method to train a fully convolutional network for semantic tissue segmentation in histopathological images. This method relies on the benefit of unsupervised learning, in the form of image…

计算机视觉与模式识别 · 计算机科学 2020-11-26 C. T. Sari , C. Sokmensuer , C. Gunduz-Demir

State-of-the-art electron microscopes such as scanning electron microscopes (SEM), scanning transmission electron microscopes (STEM) and transmission electron microscopes (TEM) have become increasingly sophisticated. However, the quality of…

计算物理 · 物理学 2023-03-31 I. Lobato , T. Friedrich , S. Van Aert

Motivation: Serial section microscopy is an established method for detailed anatomy reconstruction of biological specimen. During the last decade, high resolution electron microscopy (EM) of serial sections has become the de-facto standard…

计算机视觉与模式识别 · 计算机科学 2016-06-20 Philipp Hanslovsky , John A. Bogovic , Stephan Saalfeld

If we are ever to unravel the mysteries of brain function at its most fundamental level, we will need a precise understanding of how its component neurons connect to each other. Electron Microscopes (EM) can now provide the nanometer…

计算机视觉与模式识别 · 计算机科学 2016-09-28 Pascal Fua , Graham Knott

We study the problem of image alignment for panoramic stitching. Unlike most existing approaches that are feature-based, our algorithm works on pixels directly, and accounts for errors across the whole images globally. Technically, we…

计算机视觉与模式识别 · 计算机科学 2019-09-04 Yuelong Li , Mohammad Tofighi , Vishal Monga

Serial section Microscopy is an established method for volumetric anatomy reconstruction. Section series imaged with Electron Microscopy are currently vital for the reconstruction of the synaptic connectivity of entire animal brains such as…

计算机视觉与模式识别 · 计算机科学 2016-06-20 Philipp Hanslovsky , John A. Bogovic , Stephan Saalfeld

Identifying complex neural circuitry from electron microscopic (EM) images may help unlock the mysteries of the brain. However, identifying this circuitry requires time-consuming, manual tracing (proofreading) due to the size and intricacy…

定量方法 · 定量生物学 2014-09-04 Stephen M. Plaza

Reconstructing a complete object from its parts is a fundamental problem in many scientific domains. The purpose of this article is to provide a systematic survey on this topic. The reassembly problem requires understanding the attributes…

计算机视觉与模式识别 · 计算机科学 2025-03-28 Jiaxin Lu , Yongqing Liang , Huijun Han , Jiacheng Hua , Junfeng Jiang , Xin Li , Qixing Huang
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