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In this work, we propose a structured illumination (SI) method based on a two-photon excitation (TPE) scanning laser beam. Advantages of TPE methods include optical sectioning, low photo-toxicity, and robustness in the face of sample…

生物物理 · 物理学 2016-09-16 Alex Bardales , Qinggele Li , Bingzhao Zhu , Xiaodong Tao , Marc Reinig , Joel Kubby

Current in vivo microscopy allows us detailed spatiotemporal imaging (3D+t) of complete organisms and offers insights into their development on the cellular level. Even though the imaging speed and quality is steadily improving,…

图像与视频处理 · 电气工程与系统科学 2020-01-31 Sourabh Bhide , Ralf Mikut , Maria Leptin , Johannes Stegmaier

A low-temperature ultra-high vacuum scanning probe microscopy (SPM) system with molecular beam epitaxy capability and optical access was conceived, built, and tested in our lab. The design of the whole system is discussed here, with special…

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

光学 · 物理学 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

This comprehensive review discusses the development of scanning electron microscopy and the application of this technology in different fields such as biology, nanobiotechnology and biomedical science. Besides being a tool for high…

定量方法 · 定量生物学 2023-11-02 Aniruddha Acharya

Microscopes have morphed from purely optical instruments into motorized, robotic machines that form images on digital sensors rather than eyeballs. This continuing trend towards automation and digitization enables many new approaches to…

其他定量生物学 · 定量生物学 2020-05-04 Raghav Chhetri , Stephan Preibisch , Nico Stuurman

The Transfer Matrix Method (TMM) has become a prominent tool for the optical simulation of thin$-$film solar cells, particularly among researchers specializing in organic semiconductors and perovskite materials. As the commercial viability…

Structured illumination microscopy (SIM) achieves doubled spatial resolution by exciting the specimen with a high-contrast, high-frequency sinusoidal pattern. Such an excitation pattern can be generated by interference between multiple…

光学 · 物理学 2020-07-15 Meiqi Li , Yaning Li , Wenhui Liu , Karl Zhanghao , Peng Xi

In this paper, we have designed a low-cost scanning tunneling microscope (STM) priced at 300 USD or 2000 CNY. This microscope is suitable for educational purposes and low-demand research imaging at the nanometer level. This microscope's…

仪器与探测器 · 物理学 2024-01-17 Weilin Ma

In recent years, image-scanning microscopy (ISM, also termed pixel-reassignment microscopy) has emerged as a technique that improves the resolution and signal-to-noise compared to confocal and widefield microscopy by employing a detector…

光学 · 物理学 2023-04-19 Tal I. Sommer , Gil Weinberg , Ori Katz

This document explores the advantages of integrating open source software and practices in managing a scientific lab, emphasizing reproducibility and the avoidance of pitfalls. It details practical applications from website management using…

计算机与社会 · 计算机科学 2024-05-14 Julien Cohen-Adad

Real time computer control is an essential feature of scanning probe microscopes, which have become essential tools for the characterization and investigation of nanometer scale samples. Most commercial (and some open-source) scanning probe…

介观与纳米尺度物理 · 物理学 2013-01-24 Venkat Chandrasekhar , Manan Mehta

A mini-PET style detector system is being developed for a plant imaging application with a compact array of silicon photomultipliers (SiPM) replacing position sensitive photomultipliers (PSPMT). In addition to compactness, the use of SiPMs…

Optimizing communication performance is imperative for large-scale computing because communication overheads limit the strong scalability of parallel applications. Today's network cards contain rather powerful processors optimized for data…

分布式、并行与集群计算 · 计算机科学 2017-10-20 Torsten Hoefler , Salvatore Di Girolamo , Konstantin Taranov , Ryan E. Grant , Ron Brightwell

Light-sheet fluorescence microscopy (LSFM) is used to capture volume images of biological specimens. It offers high contrast deep inside densely fluorescence labelled samples, fast acquisition speed and minimal harmful effects on the…

图像与视频处理 · 电气工程与系统科学 2024-04-05 Niklas Rottmayer , Claudia Redenbach , Florian Fahrbach

In the era of large time-domain spectro-photometric surveys, surface variations such as starspots, chemical inhomogeneities, pulsations, rotational distortions, and binary interactions can now be directly detected and modelled. Accurately…

太阳与恒星天体物理 · 物理学 2025-11-17 M. Jabłońska , T. Różański , L. Casagrande , H. Shah , P. A. Kołaczek-Szymański , M. Rychlicki , Yuan-Sen Ting

Wireless networks are a common place nowadays and almost all of the modern devices support wireless communication in some form. These networks differ from more traditional computing systems due to the ad-hoc and spontaneous nature of…

网络与互联网体系结构 · 计算机科学 2010-07-15 Yasir Arfat Malkani , Lachhman Das Dhomeja

As an analogue of electromagnetically induced transparency (EIT), plasmon-induced transparency (PIT) has been realized both in plasmonic metamaterial and waveguide structures. Via near-field coupling within unit cells, PIT with broadband…

Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for quantitative fluorescence imaging because fluorescence lifetime is independent of concentration of fluorescent molecules or excitation/detection efficiency and is robust…

Most modern optical display and sensing devices utilize a limited number of spectral units within the visible range, based on human color perception. In contrast, the rapid advancement of machine-based pattern recognition and spectral…