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We present a new technique that combines off-axis Digital Holography and Dark Field Microscopy to track 100nm gold particles diffusing in water. We show that a single hologram is sufficient to localize several particles in a thick sample…

光学 · 物理学 2015-06-03 Frédéric Verpillat , Fadwa Joud , Pierre Desbiolles , Michel Gross

This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40…

In-line digital holography is a valuable tool for sizing, locating and tracking micro- or nano-objects in a volume. When a parametric imaging model is available, Inverse Problems approaches provide a straightforward estimate of the object…

仪器与探测器 · 物理学 2023-07-19 Nicolas Verrier , Corinne Fournier , Thierry Fournel

The 3D structure of light scattering from dark-field illuminated live 3T3 cells marked with 40 nm gold nanomarkers is explored. For this purpose, we use a high resolution holographic microscope combining the off-axis heterodyne geometry and…

We report experimental results on heterodyne holographic microscopy of subwavelength-sized gold particles. The apparatus uses continuous green laser illumination of the metal beads in a total internal reflection configuration for dark-field…

Tracking nanoparticle movement is highly desirable in many scientific areas, and various imaging methods have been employed to achieve this goal. Interferometric scattering (iSCAT) microscopy has been particularly successful in combining…

光学 · 物理学 2024-01-24 Kiarash Kasaian , Mahdi Mazaheri , Vahid Sandoghdar

In this paper, we explore the 3D structure of light scattering from dark-field illuminated live 3T3 cells in the presence of 40 nm gold nanomarkers. For this purpose, we use a high resolution holographic microscope combining the off-axis…

We use digital holographic microscopy and Mie scattering theory to simultaneously characterize and track individual colloidal particles. Each holographic snapshot provides enough information to measure a colloidal sphere's radius and…

Three-dimension real-time tracking of single emitters is an emerging tool for assessment of biological behavior as intraneuronal transport, for which spatiotemporal resolution is crucial to understand the microscopic interactions between…

We investigated the localized electronic properties of nanoporous gold films by using an ultra-high vacuum scanning tunneling microscope at low temperature (4.2 K). Second derivative scanning tunneling spectroscopy shows the plasmon peaks…

材料科学 · 物理学 2014-06-10 H. W. Liu , R. Nishitani , T. Fujita , W. Li , L. Zhang , X. Y. Lang , P. Richard , K. S. Nakayama , X. Chen , M. W. Chen , Q. K. Xue

Specialized applications of nanoparticles often call for particular, well-characterized particle size distributions in solution. But, this property can prove difficult to measure. High-throughput methods, such as dynamic light scattering,…

介观与纳米尺度物理 · 物理学 2021-09-24 Luke Melo , Angus Hui , Matt Kowal , Eric Boateng , Zahra Poursorkh , Edène Rocheron , Jake Wong , Ashton Christy , Edward Grant

Monitoring chemical reactions in solutions at the scale of individual entities is challenging: single particle detection requires small confocal volumes which are hardly compatible with Brownian motion, particularly when long integration…

Advanced three-dimensional (3D) tracking methods are essential for studying particle dynamics across a wide range of complex systems, including multiphase flows, environmental and atmospheric sciences, colloidal science, biological and…

流体动力学 · 物理学 2024-12-25 Shyam Kumar M , Jiarong Hong

A differential interference contrast microscopy technique, which employs a photonic crystal fiber as a white-light source, is used to measure both the real and imaginary parts of the complex dielectric constant of single 10 and 15 nm gold…

材料科学 · 物理学 2009-11-11 Patrick Stoller , Volker Jacobsen , Vahid Sandoghdar

Color encoding of depth is shown to occur naturally in holograms that are reconstructed under white light illumination. It can be registered in a common color photograph, allowing a simple method of visual decoding by means of ordinary…

光学 · 物理学 2015-06-26 J. J. Lunazzi

We present recent advances in the instrumentation and analysis methods for quantitative imaging of concentrated colloidal suspensions under flow. After a brief review of colloidal imaging, we describe various flow geometries for two and and…

软凝聚态物质 · 物理学 2009-09-29 Lucio Isa , Rut Besseling , Eric R. Weeks , Wilson C. K. Poon

Particle size measurement based on digital holography with conventional algorithms are usually time-consuming and susceptible to noises associated with hologram quality and particle complexity, limiting its usage in a broad range of…

应用物理 · 物理学 2020-01-01 Siyao Shao , Kevin Mallery , Jiarong Hong

Identifying, visualising and ultimately tracking dynamically moving non-fluorescent nanoparticles in the presence of non-specific scattering is a long-standing challenge across the nano- and life-sciences. In this work we demonstrate that…

光学 · 物理学 2021-08-12 Matz Liebel , Franco V. A. Camargo , Giulio Cerullo , Niek F. van Hulst

A simple and highly reproducible technique is demonstrated for the fabrication of metallic electrodes with nanometer separation. Commercially available bare gold colloidal nanoparticles are first trapped between prefabricated…

介观与纳米尺度物理 · 物理学 2009-11-07 Saiful I. Khondaker , Zhen Yao
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