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Twisting DNA under a constant applied force reveals a thermally activated transition into a state with a supercoiled structure known as a plectoneme. Using transition state theory, we predict the rate of this plectoneme nucleation to be of…

生物物理 · 物理学 2015-03-17 Bryan C. Daniels , James P. Sethna

We observed reptation of single DNA molecules in fused silica nanoslits of sub-30 nm height. The reptation behavior and the effect of confinement are quantitatively characterized using orientation correlation and transverse fluctuation…

生物物理 · 物理学 2015-02-19 Jia-Wei Yeh , K. K. Sriram , Alessandro Taloni , Yeng-Long Chen , Chia-Fu Chou

Bending of DNA from a straight rod to a circular form in presence of any of the mono-, di-, tri- or tetravalent counterions has been simulated in strong Coulomb coupling environment employing a previously developed energy minimization…

生物物理 · 物理学 2015-05-28 Arup K. Mukherjee

RNA folding is a kinetic process governed by the competition of a large number of structures stabilized by the transient formation of base pairs that may induce complex folding pathways and the formation of misfolded structures. Despite of…

生物物理 · 物理学 2009-03-16 M. Manosas , I. Junier , F. Ritort

The influence of tension on DNA looping has been studied both experimentally and theoretically in the past. However, different theoretical models have yielded different predictions, leaving uncertainty about their validity. We briefly…

统计力学 · 物理学 2025-01-17 Wout Laeremans , Wouter G Ellenbroek

The aim of the present paper is the study of the entropic elasticity of the dsDNA molecule, having a cristallographic length L of the order of 10 to 30 persistence lengths A, when it is subject to spatial obstructions. We have not tried to…

软凝聚态物质 · 物理学 2009-11-11 C. Bouchiat

DNA polymerases are an essential class of enzymes or molecular motors that catalyze processive DNA syntheses during DNA replications. A critical issue for DNA polymerases is their molecular mechanism of processive DNA replication. We have…

软凝聚态物质 · 物理学 2021-11-01 Yu-Ru Liu , Peng-Ye Wang , Wei Li , Ping Xie

We study the electric properties of single-stranded DNA molecules with hairpin-like shapes in the presence of a magnetic flux. It is shown that the current amplitude can be modulated by the applied field. The details of the electric…

介观与纳米尺度物理 · 物理学 2007-05-23 Juyeon Yi , Henri Orland

Biological information is not only stored in the digital chemical sequence of double helical DNA, but is also encoded in the mechanical properties of the DNA strands, which can influence biochemical processes involving its readout. For…

软凝聚态物质 · 物理学 2015-06-10 Christian Matek , Thomas E. Ouldridge , Jonathan P. K. Doye , Ard A. Louis

We examine the conformations of a model for a short segment of closed DNA. The molecule is represented as a cylindrically symmetric elastic rod with a constraint corresponding to a specification of the linking number. We obtain analytic…

软凝聚态物质 · 物理学 2009-10-31 Boris Fain , Joseph Rudnick

Topology affects physical and biological properties of DNA and impacts fundamental cellular processes, such as gene expression, genome replication, chromosome structure and segregation. In all organisms DNA topology is carefully modulated…

生物大分子 · 定量生物学 2019-03-27 P. Bettotti , V. Visone , L. Lunelli , G. Perugino , M. Ciaramella , A. Valenti

Fractal-like nanopatterned DNA thin films have been fabricated on mica substrate by Langmuir-Blodgett (LB) technique. Structures and components of DNA nanopatterns were investigated using atomic force microscopy (AFM) and X-ray…

材料科学 · 物理学 2010-09-10 Shuxi Dai , Xingtang Zhang , Zuliang Du , Hongxin Dang

Motivated by the wide ranging experimental results on the conductivity of DNA, we have investigated extraordinary configurations and chemical environments in which DNA might become a true molecular wire, perticularly from enhanced…

软凝聚态物质 · 物理学 2009-09-29 R. G. Endres , D. L. Cox , R. R. P. Singh

A solid-state nanopore can electrophoretically capture a DNA molecule and pull it through in a folded configuration. The resulting ionic current signal indicates where along its length the DNA was captured. A statistical study using an 8 nm…

生物物理 · 物理学 2015-06-11 Mirna Mihovilovic , Nick Hagerty , Derek Stein

Atomic Force Microscopy analysis is employed to study the geometrical and topological properties of $3000$kbp DNA molecules fixed in mica substrates with $MgCl_{2}$. We found that the aggregates on the substrate surface for certain salt…

软凝聚态物质 · 物理学 2017-06-09 Elsa de la Calleja , R. F Bazoni , M. S. Rocha , Marcia Barbosa

Enzymatic ligation is essential for the synthesis of long DNA. However, the number of ligated products exponentially decays as the DNA synthesis proceeds in a random manner. The controlling of ligation randomness is of importance to…

生物物理 · 物理学 2021-09-29 Takaharu Y. Shiraki , Ken-ichiro Kamei , Yusuke T. Maeda

From a nanoscience perspective, cellular processes and their reduced in vitro imitations provide extraordinary examples for highly robust few or single molecule reaction pathways. A prime example are biochemical reactions involving DNA…

生物物理 · 物理学 2007-05-23 Ralf Metzler , Tobias Ambjoernsson , Andreas Hanke , Yongli Zhang , Stephen Levene

This paper introduces the use of cable dynamics models as a means to explore the mechanics of DNA on long-length scales. It is on these length scales that DNA forms twisted and curved three-dimensional shapes known as supercoils and loops.…

生物物理 · 物理学 2007-05-23 Sachin Goyal , Todd Lillian , Noel C. Perkins , Edgar Meyhofer

The flexibility of short DNA chains is investigated via computation of the average correlation function between dimers which defines the persistence length. Path integration techniques have been applied to confine the phase space available…

软凝聚态物质 · 物理学 2018-10-19 Marco Zoli

Trapping, linearization, and imaging of single molecule DNA is of broad interest to both biophysicists who study polymer physics and engineers who build nucleic acid analyzing methods such as optical mapping. In this study, single DNA…