激光扫描显微镜中驻波激发的多平面荧光揭示红细胞三维信息
生物物理
2015-01-05 v2 光学
摘要
荧光驻波激发在光学显微镜中备受推崇，因为它能提高轴向分辨率。我们在此证明，通过在样品附近放置平面反射镜，可在单点激光扫描显微镜中产生由驻波激发的多平面荧光。我们报告称，每个激发平面内的相对强度取决于荧光团的斯托克斯位移。我们通过使用针对细胞膜的特异性染料，展示了如何利用驻波激发生成红细胞表面膜的精确等高线图，其轴向分辨率约为 90 nm。该方法仅需在现有的共聚焦激光扫描显微镜中添加一面平面镜，可能在研究涉及红细胞膜的疾病（如疟疾）方面证明是有用的。
引用
@article{arxiv.1408.2958,
title = {Standing-wave-excited multiplanar fluorescence in a laser scanning microscope reveals 3D information on red blood cells},
author = {Rumelo Amor and Sumeet Mahajan and William Bradshaw Amos and Gail McConnell},
journal= {arXiv preprint arXiv:1408.2958},
year = {2015}
}
备注
15 pages, 4 figures; changed the discussion of narrow-band detected fringes (Fig. 3) to describe the phenomenon as a moire pattern between the excitation and emission standing-wave fields, rather than a beats pattern; added DiI(5)-labelled red blood cell in Fig. 4 to show that standing-wave fringes are present even when the dye excitation wavelength is outside the haemoglobin absorption band