用于细胞膜成像的共焦超临界角荧光显微镜
光学
2016-11-25 v1
摘要
我们展示了利用传统共焦显微镜对生物样品进行亚波长层析成像。这种光学层析是通过超临界角荧光现象实现的，其中只有位于折射率不连续界面附近的荧光团才能将辐射的传播分量发射到所谓的“禁戒角”中。该技术的简便性使其仅需对探测通道进行简单修改，即可集成到高数值孔径共焦扫描显微镜中。共焦 -SAF 显微镜将成为实现高分辨率表面成像的有力工具，尤其适用于生物样品的膜成像。
引用
@article{arxiv.1312.6294,
title = {Confocal supercritical angle fluorescence microscopy for cell membrane imaging},
author = {Siddharth Sivankutty and Thomas Barroca and Céline Mayet and Guillaume Dupuis and Emmanuel Fort and Sandrine Lévêque-Fort},
journal= {arXiv preprint arXiv:1312.6294},
year = {2016}
}
备注
This paper was published in Optics Letters and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following link on the OSA website: http://www.opticsinfobase.org/ol/upcoming_pdf.cfm?id=200895. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law