Correction of cell-induced optical aberrations in a fluorescence fluctuation microscope
Biological Physics
2014-03-13 v1 Optics
Abstract
We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive tool to assess the optical aberrations introduced by the cell. We demonstrate an adaptive optics (AO) system to remove the aberration-related bias in the FFM measurements. Our data show that AO is not only useful when imaging deep in tissues but also when performing FFM measurements through a single cellular layer. This work paves the way for the application of FFM to complex three-dimensional multicellular samples.
Cite
@article{arxiv.1403.2833,
title = {Correction of cell-induced optical aberrations in a fluorescence fluctuation microscope},
author = {Charles-Edouard Leroux and Alexei Grichine and Irène Wang and Antoine Delon},
journal= {arXiv preprint arXiv:1403.2833},
year = {2014}
}