Related papers: Correction of cell-induced optical aberrations in …
Adaptive optics (AO) is a powerful image correction technique with proven benefits for many life-science microscopy methods. However, the complexity of adding a reflective wavefront modulator and a wavefront sensor into already complicated…
Fluorescence Correlation Spectroscopy (FCS) yields measurement parameters (number of molecules, diffusion time) that characterize the concentration and kinetics of fluorescent molecules within a supposedly known observation volume. Absolute…
Multifocal plane microscopy (MUM) allows three dimensional objects to be imaged in a single camera frame. Our approach uses dual orthogonal diffraction phase gratings with a quadratic distortion of the lines to apply defocus to the first…
Three-photon (3-P) fluorescence microscopy enables deep in vivo imaging with subcellular resolution, but its performance is fundamentally constrained by the maximum permissible laser power required to avoid tissue heating and photodamage.…
Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…
The resolution and contrast of microscope imaging is often affected by aberrations introduced by imperfect optical systems and inhomogeneous refractive structures in specimens. Adaptive optics (AO) compensates these aberrations and restores…
FRET measurements can provide dynamic spatial information on length scales smaller than the diffraction limit of light. Several methods exist to measure FRET between fluorophores, including Fluorescence Lifetime Imaging Microscopy (FLIM),…
Future large space telescopes will be equipped with adaptive optics (AO) to overcome wavefront aberrations and achieve high contrast for imaging faint astronomical objects, such as earth-like exoplanets and debris disks. In contrast to AO…
Adaptive optics (AO) has revolutionized imaging in {fields} from astronomy to microscopy by correcting optical aberrations. In label-free microscopes, however, conventional AO faces limitations due to the absence of guidestar and the need…
The random motion of molecules in living cells has consistently been reported to deviate from standard Brownian motion, a behavior coined as ``anomalous diffusion''. Fluorescence Correlation Spectroscopy (FCS) is a powerful method to…
Fluorescence Correlation Spectroscopy (FCS) is a powerful single-molecule technique which allows for measuring motion (diffusion, flow), concentration, and molecular interaction kinetics of fluorescent molecules from picomolar to micromolar…
Images obtained by microscopes are generally degraded by aberrations. Adaptive optics (AO) has been widely used in to compensate for aberrations and improve reduced image quality. Requiring no separate wavefront sensor, sensorless AO…
The imaging performance of an optical microscope can be degraded by sample-induced aberrations. A general strategy to undo the effect of these aberrations is to apply wavefront correction with a deformable mirror (DM). In most cases, the DM…
A fundamental parameter to determine how electromagnetic waves interfere is their relative phase. Therefore, achieving a fine control over it enables a wide range of interferometric applications. Existing phase control methods rely on…
The aim of this paper is to provide a mathematical model for spatial distribution of membrane electrical potential changes by fluorescence diffuse optical tomography. We derive the resolving power of the imaging method in the presence of…
Adaptive optics (AO) is a powerful tool employed across various research fields, from aerospace to microscopy. Traditionally, AO has focused on correcting optical phase aberrations, with recent advances extending to polarisation…
Fluorescence fluctuations-based super-resolution microscopy (FF-SRM) is an emerging field promising low-cost and live-cell compatible imaging beyond the resolution of conventional optical microscopy. A comprehensive overview on how the…
Context. Adaptive optics (AO) is now a tool commonly deployed in astronomy. The real time correction of the atmospheric turbulence that AO enables allows telescopes to perform close to the diffraction limit at the core of their point spread…
Light microscopy enables multifunctional imaging of biological specimens at unprecedented depths and resolutions. However, the performance of all optical methods degrades with the imaging depth due to sample-induced aberrations. Methods of…
The life of a cell is governed by highly dynamical microscopic processes. Two notable examples are the diffusion of membrane receptors and the kinetics of transcription factors governing the rates of gene expression. Different fluorescence…