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Interference of light fields plays an important role in various high-precision measurement schemes. It has been shown that super resolving phase measurements beyond the standard coherent state limit can be obtained either by using maximally…

Quantum Physics · Physics 2013-10-08 Emanuele Distante , Miroslav Jezek , Ulrik L. Andersen

Wavefront-shaping is a promising approach for imaging fluorescent targets deep inside scattering tissue despite strong aberrations. It enables focusing an incoming illumination into a single spot inside tissue, as well as correcting the…

Optics · Physics 2024-01-23 Dror Aizik , Anat Levin

Information about microscopic objects with features smaller than the diffraction limit is almost entirely lost in a far-field diffraction image but could be partly recovered with data completition techniques. Any such approach critically…

Optics · Physics 2020-11-17 Maria Bancerek , Krzysztof M. Czajkowski , Rafal Kotynski

In single-molecule microscopy it is necessary to locate with high precision point sources from noisy observations of the spectrum of the signal at frequencies capped by $f_c$, which is just about the frequency of natural light. This paper…

Information Theory · Computer Science 2015-04-06 Veniamin I. Morgenshtern , Emmanuel J. Candes

An image super-resolution method from multiple observation of low-resolution images is proposed. The method is based on sub-pixel accuracy block matching for estimating relative displacements of observed images, and sparse signal…

Computer Vision and Pattern Recognition · Computer Science 2014-02-18 Toshiyuki Kato , Hideitsu Hino , Noboru Murata

In single-molecule super-resolution microscopy, engineered point-spread functions (PSFs) are designed to efficiently encode new molecular properties, such as 3D orientation, into complex spatial features captured by a camera. To fully…

Image and Video Processing · Electrical Eng. & Systems 2019-07-16 Hesam Mazidi , Eshan S. King , Oumeng Zhang , Arye Nehorai , Matthew D. Lew

We demonstrate single-site-resolved fluorescence imaging of ultracold $^{87}\mathrm{Rb}$ atoms in a triangular optical lattice by employing Raman sideband cooling. Combining a Raman transition at the D1 line and a photon scattering through…

Quantum Gases · Physics 2020-12-22 Ryuta Yamamoto , Hideki Ozawa , David C. Nak , Ippei Nakamura , Takeshi Fukuhara

Microscopy is one of the most essential imaging techniques in life sciences. High-quality images are required in order to solve (potentially life-saving) biomedical research problems. Many microscopy techniques do not achieve sufficient…

Computer Vision and Pattern Recognition · Computer Science 2018-10-24 Joris Roels , Jan Aelterman , Jonas De Vylder , Hiep Luong , Yvan Saeys , Wilfried Philips

We have developed an automatic method for segmenting fluorescence lifetime (FLT) imaging microscopy (FLIM) images of cells inspired by a multi-resolution community detection (MCD) based network segmentation method. The image processing…

Medical Physics · Physics 2014-01-06 Dandan Hu , Pinaki Sarder , Peter Ronhovde , Sandra Orthaus , Samuel Achilefu , Zohar Nussinov

Scalable atom-based quantum platforms for simulation, computing, and metrology require fast high-fidelity, low-loss imaging of individual atoms. Standard fluorescence detection methods rely on continuous cooling, limiting the detection…

Super-resolution fluorescence microscopy overcomes blurring arising from light diffraction, allowing the reconstruction of fine scale details in biological structures. Standard methods come at the expense of long acquisition time and/or…

Optimization and Control · Mathematics 2021-10-12 Bastien Laville , Laure Blanc-Féraud , Gilles Aubert

An optical imager that exploits off-center image rotation to encode both the lateral and depth coordinates of point sources in a single snapshot can perform 3D localization and tracking of space debris. When actively illuminated, unresolved…

Instrumentation and Methods for Astrophysics · Physics 2018-12-24 Chao Wang , Robert Plemmons , Sudhakar Prasad , Raymond Chan , Mila Nikolova

We show that structural information can be extracted from single molecule localization microscopy (SMLM) data. More precisely, we reinterpret SMLM data as the measures of a phaseless optical diffraction tomography system for which the…

Signal Processing · Electrical Eng. & Systems 2023-07-19 Thanh-An Pham , Emmanuel Soubies , Ferréol Soulez , Michael Unser

The knowledge of the exact structure of the optical system PSF enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications.…

Super-resolution microscopy is rapidly gaining importance as an analytical tool in the life sciences. A compelling feature is the ability to label biological units of interest with fluorescent markers in living cells and to observe them…

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

Optics · Physics 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

Nanoscale localization of point emitters is critical to several methods in optical fluorescence microscopy, including single-molecule super-resolution imaging and tracking. While the precision of the localization procedure has been the…

Optics · Physics 2020-07-15 Petar N. Petrov , W. E. Moerner

Optically active quantum dots, for instance self-assembled InGaAs quantum dots, are potentially excellent single photon sources. The fidelity of the single photons is much improved using resonant rather than non-resonant excitation. With…

Mesoscale and Nanoscale Physics · Physics 2014-10-08 Andreas V. Kuhlmann , Julien Houel , Daniel Brunner , Arne Ludwig , Dirk Reuter , Andreas D. Wieck , Richard J. Warburton

An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking…

We present the experimental reconstruction of sub-wavelength features from the far-field intensity of sparse optical objects: sparsity-based sub-wavelength imaging combined with phase-retrieval. As examples, we demonstrate the recovery of…