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In biological research, fluorescence staining is a key technique to reveal the locations and morphology of subcellular structures. However, it is slow, expensive, and harmful to cells. In this paper, we model it as a deep learning task…

Computer Vision and Pattern Recognition · Computer Science 2023-03-28 Donghao Zhou , Chunbin Gu , Junde Xu , Furui Liu , Qiong Wang , Guangyong Chen , Pheng-Ann Heng

Fluorescence microscopy has revolutionized biomedical research over the past three decades. Its high molecular specificity and unrivaled single molecule level sensitivity have enabled breakthroughs in a variety of research fields. For in…

Optics · Physics 2012-03-21 Meng Cui , Ke Si , Reto Fiolka

We proposed a method to achieve superresolved optical imaging without beating the diffraction limit of light. This is achieved by magnifying the ideal optical image of the object through higher-order spatial frequency generation while…

Optics · Physics 2016-08-08 Zhixiang Li , Jianji Liu , Guoquan Zhang

In many real-world problems, we are dealing with collections of high-dimensional data, such as images, videos, text and web documents, DNA microarray data, and more. Often, high-dimensional data lie close to low-dimensional structures…

Computer Vision and Pattern Recognition · Computer Science 2013-02-06 Ehsan Elhamifar , Rene Vidal

3D super-resolution fluorescence microscopy typically requires sophisticated setups, sample preparation, or long measurements. A notable exception, SOFI, only requires recording a sequence of frames and no hardware modifications whatsoever…

Optics · Physics 2024-03-01 Pawel Szczypkowski , Monika Pawlowska , Radek Lapkiewicz

Single fluorescent molecules, behaving as ideal electric dipole emitters, are powerful nanoscopic probes of complex optical fields. Here, this property is exploited to precisely map the polarization and vectorial structure of tightly…

In this two-part paper, we present a novel framework and methodology to analyze data from certain image-based biochemical assays, e.g., ELISPOT and Fluorospot assays. In this second part, we focus on our algorithmic contributions. We…

Signal Processing · Electrical Eng. & Systems 2018-10-19 Pol del Aguila Pla , Joakim Jaldén

Super-resolution microscopy has revolutionized optical fluorescence imaging by improving 3D resolution by 1-2 orders of magnitude. While different methods can successfully increase the resolution, all methods share significant differences…

Biological Physics · Physics 2015-01-26 Thomas Pengo , Nicolas Olivier , Suliana Manley

Sparsity-constrained optimization underlies many problems in signal processing, statistics, and machine learning. State-of-the-art hard-thresholding (HT) algorithms rely on an appropriately selected continuous step-size parameter to ensure…

Machine Learning · Statistics 2026-05-13 Jin Zhu , Junxian Zhu , Zezhi Wang , Borui Tang , Hongmei Lin , Xueqin Wang

The analysis of entangled atomic ensembles and their application for interferometry beyond the standard quantum limit requires an accurate determination of the number of atoms. We present an accurate fluorescence detection technique for…

Quantum Physics · Physics 2019-12-13 A. Hüper , C. Pür , M. Hetzel , J. Geng , J. Peise , I. Kruse , M. Kristensen , W. Ertmer , J. Arlt , C. Klempt

Highly clumped nuclei clusters captured in fluorescence in situ hybridization microscopy images are common histology entities under investigations in a wide spectrum of tissue-related biomedical investigations. Due to their large scale in…

Computer Vision and Pattern Recognition · Computer Science 2018-08-15 Xiaoyuan Guo , Hanyi Yu , Blair Rossetti , George Teodoro , Daniel Brat , Jun Kong

Super-resolution effects in optical and fluorescence biological systems are analyzed and their relations with uncertainty relations are discussed. Super-resolutions obtained in the optical systems, including especially NSOM, SIM and…

Optics · Physics 2015-06-12 Yacob Ben-Aryeh

Photonic circuits can be much faster than their electronic counterparts, but they are difficult to miniaturize below the optical wavelength scale. Nanoscale photonic circuits based on surface plasmon polaritons (SPs) are a promising…

In this paper, we consider a well-known sparse optimization problem that aims to find a sparse solution of a possibly noisy underdetermined system of linear equations. Mathematically, it can be modeled in a unified manner by minimizing…

Optimization and Control · Mathematics 2021-10-01 Lei Yang , Xiaojun Chen , Shuhuang Xiang

Solid-state single-photon emitters (SPE) are a basis for emerging technologies such as quantum communication and quantum sensing. SPE based on fluorescent point defects are ubiquitous in semiconductors and insulators, and new systems with…

Mesoscale and Nanoscale Physics · Physics 2023-11-28 Leah R. Narun , Rebecca E. K. Fishman , Henry J. Shulevitz , Raj N. Patel , Lee C. Bassett

We present new advances in the spectral extraction of point-like sources adapted to the Infrared Spectrograph onboard the Spitzer Space Telescope. For the first time, we created a super-sampled point spread function of the low-resolution…

Instrumentation and Methods for Astrophysics · Physics 2015-05-14 V. Lebouteiller , J. Bernard-Salas , G. C. Sloan , D. J. Barry

Diffusion models are a powerful framework for tackling ill-posed problems, with recent advancements extending their use to point cloud upsampling. Despite their potential, existing diffusion models struggle with inefficiencies as they map…

Computer Vision and Pattern Recognition · Computer Science 2025-01-28 Zhi-Song Liu , Chenhang He , Lei Li

Many interesting and fundamentally practical optimization problems, ranging from optics, to signal processing, to radar and acoustics, involve constraints on the Fourier transform of a function. It is well-known that the {\em fast Fourier…

Optimization and Control · Mathematics 2012-09-05 Robert J. Vanderbei

Strong gravitational lensing offers a wealth of astrophysical information on the background source it affects, provided the lensed source can be reconstructed as if it was seen in the absence of lensing. In the present work, we illustrate…

Instrumentation and Methods for Astrophysics · Physics 2019-02-27 R. Joseph , F. Courbin , J. -L. Starck , S. Birrer

Imaging below the diffraction limit is always a public interest because of the restricted resolution of conventional imaging systems. To beat the limit, evanescent harmonics decaying in space must participate in the imaging process. Here,…

Optics · Physics 2019-10-02 Tie-Jun Huang , Li-Zheng Yin , Ya Shuang , Jiang-Yu Liu , Yunhua Tan , Pu-Kun Liu
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