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Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field…

Deblurring is a fundamental inverse problem in bioimaging. It requires modelling the point spread function (PSF), which captures the optical distortions entailed by the image formation process. The PSF limits the spatial resolution…

Image and Video Processing · Electrical Eng. & Systems 2019-03-04 Denis K. Samuylov , Prateek Purwar , Gábor Székely , Grégory Paul

Stereo vision between images faces a range of challenges, including occlusions, motion, and camera distortions, across applications in autonomous driving, robotics, and face analysis. Due to parameter sensitivity, further complications…

Computer Vision and Pattern Recognition · Computer Science 2026-01-21 Antonin Clerc , Michael Quellmalz , Moritz Piening , Philipp Flotho , Gregor Kornhardt , Gabriele Steidl

In traditional optical imaging systems, the spatial resolution is limited by the physics of diffraction, which acts as a low-pass filter. The information on sub-wavelength features is carried by evanescent waves, never reaching the camera,…

Optics · Physics 2018-12-13 Oren Solomon , Yonina C. Eldar , Maor Mutzafi , Mordechai Segev

Optical super-resolution microscopy is a key technology for structural biology that offers high imaging contrast and live-cell compatibility. Minimal (fluorescence) photons flux microscopy, or MINFLUX, is an emerging super-resolution…

Optics · Physics 2025-08-29 Lukas Scheiderer , Zach Marin , Jonas Ries

Localization microscopy enables imaging with resolutions that surpass the conventional optical diffraction limit. Notably, the MINFLUX method achieves super-resolution by shaping the excitation point-spread function (PSF) to minimize the…

Recording the fluorescence of a magneto-optical trap (MOT) is a standard tool for measuring atom numbers in experiments with ultracold atoms. When trapping few atoms in a small MOT, the emitted fluorescence increases with the atom number in…

Quantum Gases · Physics 2021-03-17 Martin Schlederer , Alexandra Mozdzen , Thomas Lompe , Henning Moritz

Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background…

Optics · Physics 2021-02-02 Oumeng Zhang , Matthew D. Lew

MINFLUX (Minimal Photon Flux) is a single-molecule imaging technique capable of resolving fluorophores at a precision of <5 nm. Interpretation of the point patterns generated by this technique presents challenges due to variable emitter…

Applications · Statistics 2026-02-24 Jack Peyton , Benjamin Davis , Emily Gribbin , Daniel Rolfe , Hannah Mitchell

This paper studies the problem of exact localization of sparse (point or extended) objects with noisy data. The crux of the proposed approach consists of random illumination. Several recovery methods are analyzed: the Lasso, BPDN and the…

Information Theory · Computer Science 2015-05-19 Albert Fannjiang

Motivated by the importance of optical microscopes to science and engineering, scientists have pondered for centuries how to improve their resolution and the existence of fundamental resolution limits. In recent years, a new class of…

Quantum Physics · Physics 2015-07-28 Mankei Tsang

Single-sample face recognition is one of the most challenging problems in face recognition. We propose a novel algorithm to address this problem based on a sparse representation based classification (SRC) framework. The new algorithm is…

Computer Vision and Pattern Recognition · Computer Science 2014-02-11 Liansheng Zhuang , Tsung-Han Chan , Allen Y. Yang , S. Shankar Sastry , Yi Ma

Here, we report analysis and summary of research in the field of localization microscopy for optical imaging. We introduce the basic elements of super-resolved localization microscopy methods for PALM and STORM, commonly used both in vivo…

Optics · Physics 2021-02-25 Jack W Shepherd , Mark C Leake

In the past years, optical fluorescence microscopy (OFM) made steady progress towards increasing the localisation precision of fluorescent emitters in biological samples. The high precision achieved by these techniques has prompted new…

We present an ultra-fast, precise, parameter-free method, which we term Deep-STORM, for obtaining super-resolution images from stochastically-blinking emitters, such as fluorescent molecules used for localization microscopy. Deep-STORM uses…

Optics · Physics 2018-05-03 Elias Nehme , Lucien E. Weiss , Tomer Michaeli , Yoav Shechtman

We demonstrate improved detection of small trapped atomic ensembles through advanced post-processing and optimal analysis of absorption images. A fringe removal algorithm reduces imaging noise to the fundamental photon-shot-noise level and…

Atomic Physics · Physics 2010-12-17 C. F. Ockeloen , A. F. Tauschinsky , R. J. C. Spreeuw , S. Whitlock

Single-molecule approaches to biology offer a powerful new vision to elucidate the mechanisms that underpin the functioning of living cells. However, conventional optical single molecule spectroscopy techniques such as F\"orster…

Microscopy enables direct observation of cellular morphology in 3D, with transmitted-light methods offering low-cost, minimally invasive imaging and fluorescence microscopy providing specificity and contrast. Virtual staining combines these…

Many microscopy applications are limited by the total amount of usable light and are consequently challenged by the resulting levels of noise in the acquired images. This problem is often addressed via (supervised) deep learning based…

Image and Video Processing · Electrical Eng. & Systems 2020-08-20 Anna S. Goncharova , Alf Honigmann , Florian Jug , Alexander Krull

Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…