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The capability of focus control has been central to optical technologies that require both high temporal and spatial resolutions. However, existing varifocal lens schemes are commonly limited to the response time on the microsecond…

We introduce a full field fluorescence imaging technique with axial confinement of about 100 nm at the sample/substrate interface. Contrary to standard surface imaging techniques, this confinement is obtained through emission filtering.…

Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field…

We report a new lensless microscopy configuration by integrating the concepts of transverse translational ptychography and defocus multi-height phase retrieval. In this approach, we place a tilted image sensor under the specimen for…

Image and Video Processing · Electrical Eng. & Systems 2021-11-03 Shaowei Jiang , Chengfei Guo , Patrick Hu , Derek Hu , Pengming Song , Tianbo Wang , Zichao Bian , Zibang Zhang , Guoan Zheng

Hyperspectral microscopy is an imaging technique that provides spectroscopic information with high spatial resolution. When applied in the relevant wavelength region, such as in the infrared (IR), it can reveal a rich spectral fingerprint…

Hard x-ray imaging is indispensable across diverse fields owing to its high penetrability. However, the resolution of traditional x-ray imaging modalities, such as computed tomography (CT) systems, is constrained by factors including beam…

Medical Physics · Physics 2024-02-23 O. Sefi , A. Ben Yehuda , Y. Klein , S. Bloch , H. Schwartz , E. Cohen , S. Shwartz

Aperture based scanning near field optical microscopes are important instruments to study light at the nanoscale and to understand the optical functionality of photonic nanostructures. In general, a detected image is affected by both, the…

Many experiments in biological and medical sciences currently use multiphoton microscopy as a core imaging technique. To date, solid-state lasers are most commonly used as excitation beam sources. However, the most demanding applications…

Investigation of cell structure is hardly imaginable without bright-field microscopy. Numerous modifications such as depth-wise scanning or videoenhancement make this method being state-of-the-art. This raises a question what maximal…

Image and Video Processing · Electrical Eng. & Systems 2020-10-29 Kirill Lonhus , Renata Rychtarikova , Ganna Platonova , Dalibor Stys

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…

Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that…

Biological Physics · Physics 2019-11-06 Jiaming Guo , Camille Artur , Jason L. Eriksen , David Mayerich

Light field (LF) image super-resolution (SR) aims at reconstructing high-resolution LF images from their low-resolution counterparts. Although CNN-based methods have achieved remarkable performance in LF image SR, these methods cannot fully…

Computer Vision and Pattern Recognition · Computer Science 2022-01-31 Zhengyu Liang , Yingqian Wang , Longguang Wang , Jungang Yang , Shilin Zhou

Nonlinear optical microscopy allows rapid high-resolution microscopy with image contrast generated from intrinsic properties of the sample. Established modalities such as multiphoton excited fluorescence and second/third-harmonic generation…

Super-resolution fluorescence microscopy is of great interest in life science studies for visualizing subcellular structures at the nanometer scale. Among various kinds of super-resolution approaches, image scanning microscopy (ISM) offers…

A novel THz near-field spectrometer is presented which allows to perform biological and medical studies with high spectral resolution combined with a spatial resolution down to l/100. In the setup an aperture much smaller than the used…

Biological Physics · Physics 2009-11-07 S. Mair , B. Gompf , M. Dressel

We demonstrate a significant resolution enhancement beyond the conventional limit in multiphoton microscopy (MPM) using saturated excitation of fluorescence. Our technique achieves super-resolved imaging by temporally modulating the…

Optical stellar interferometers have demonstrated milli-arcsecond resolution with few apertures spaced hundreds of meters apart. To obtain rich direct images, many apertures will be needed, for a better sampling of the incoming wavefront.…

Instrumentation and Methods for Astrophysics · Physics 2014-09-17 Arun Surya , Swapan K. Saha , Antoine Labeyrie

This work demonstrates a multi-lens microscopic imaging system that overlaps multiple independent fields of view on a single sensor for high-efficiency automated specimen analysis. Automatic detection, classification and counting of various…

Computer Vision and Pattern Recognition · Computer Science 2022-01-19 Xing Yao , Vinayak Pathak , Haoran Xi , Amey Chaware , Colin Cooke , Kanghyun Kim , Shiqi Xu , Yuting Li , Timothy Dunn , Pavan Chandra Konda , Kevin C. Zhou , Roarke Horstmeyer

In this work, we propose using camera arrays coupled with coherent illumination as an effective method of improving spatial resolution in long distance images by a factor of ten and beyond. Recent advances in ptychography have demonstrated…

Computer Vision and Pattern Recognition · Computer Science 2015-11-29 Jason Holloway , M. Salman Asif , Manoj Kumar Sharma , Nathan Matsuda , Roarke Horstmeyer , Oliver Cossairt , Ashok Veeraraghavan

We report a novel lensless on-chip microscopy platform based on near-field blind ptychographic modulation. In this platform, we place a thin diffuser in between the object and the image sensor for light wave modulation. By blindly scanning…

Image and Video Processing · Electrical Eng. & Systems 2020-02-13 Shaowei Jiang , Jiakai Zhu , Pengming Song , Chengfei Guo , Zichao Bian , Ruihai Wang , Yikun Huang , Shiyao Wang , He Zhang , Guoan Zheng
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