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The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. We focus here on computational methods that promise to boost live-cell fluorescence microscopy,…
We present an analytical description and an experimental realization of interscale mixing microscopy, a diffraction-based imaging technique that is capable of detecting wavelength/10 objects in far-field measurements with both coherent and…
Spectral imaging is a fundamental diagnostic technique with widespread application. Conventional spectral imaging approaches have intrinsic limitations on spatial and spectral resolutions due to the physical components they rely on. To…
A number of questions in systems biology such as understanding how dynamics of neuronal networks are related to brain function require the ability to capture the functional dynamics of large cellular populations at high speed. Recently,…
The most realistic information about the transparent sample such as a live cell can be obtained only using bright-field light microscopy. At high-intensity pulsing LED illumination, we captured a primary 12-bit-per-channel (bpc) response…
Recent development of lensless imagers has enabled three-dimensional (3D) imaging through a thin piece of optics in close proximity to a camera sensor. A general challenge of wide-field lensless imaging is the high computational complexity…
In this short review we will highlight some of the recent advancements in the field of high-resolution laboratory spectroscopy that meet the needs dictated by the advent of highly sensitive and broadband telescopes like ALMA and SOFIA.…
High-speed image acquisition in light microscopy is essential for a wide range of applications, including observing dynamic biological processes and enabling high-throughput sample analysis. However, traditional imaging speeds are often…
Structured Illumination Microscopy is a widespread methodology to image live and fixed biological structures smaller than the diffraction limits of conventional optical microscopy. Using recent advances in image up-scaling through deep…
We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is…
With the introduction of consumer light field cameras, light field imaging has recently become widespread. However, there is an inherent trade-off between the angular and spatial resolution, and thus, these cameras often sparsely sample in…
Ptychography has become prominent at synchrotron facilities worldwide for characterizing biological and material specimens' topological structures and properties at the nanometer or atomic scale, due to its lens - less, highly quantitative…
Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms.…
The resolution of far-field optical microscopes, which rely on propagating optical modes, is widely believed to be limited because of diffraction to a value on the order of a half-wavelength $\lambda /2$ of the light used. Although…
Scattering and absorption limit the penetration of optical fields into tissue, but wavefront correction, often used to compensate for these effects, is incompatible with wide field-of-view imaging and complex to implement. We demonstrate a…
Optical approaches to fluorescent, spectroscopic, and morphological imaging have made exceptional advances in the last decade. Super-resolution imaging and wide-field multiphoton imaging are now underpinning major advances across the…
Light-field imaging facilitates the miniaturization of 3D cameras while it requires the extension of the depth-of-field (DoF) for practical applications such as endoscopy and intraoral scanning. Here we report a light-field camera (LFC)…
As one of important analysis tools, microscopes with high spatial resolution are indispensable for scientific research and medical diagnosis, and much attention is always focused on the improvement of resolution. Over the past decade, a…
Volumetric biological imaging often involves compromising high temporal resolution at the expense of high spatial resolution when popular scanning methods are used to capture 3D information. We introduce an integrated experimental and image…
Formation of a bright-field microscopic image of a transparent phase object is described in terms of elementary geometrical optics. Our approach is based on the premise that image replicates the intensity distribution (real or virtual) at…