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Phase microscopy is an invaluable tool in the biosciences and in clinical diagnostics. The sensitivity of current phase microscopy techniques is optimized for one specific mean phase value and varies significantly across a given sample.…

Optics · Physics 2019-08-30 Thomas Juffmann , Andrés de los Ríos Sommer , Sylvain Gigan

Speckled illumination enhances widefield fluorescence microscopy by enabling optical sectioning and super resolution. In random illumination microscopy, sequences of speckled illumination patterns are used to excite fluorescent samples and…

Recently, it was discovered that microsphere can generate super-resolution focusing beyond diffraction limit. This has led to the development of an exciting super-resolution imaging technique -microsphere nanoscopy- that features a record…

Optics · Physics 2021-08-26 Zengbo Wang

Most computer vision systems and computational photography systems are visible light based which is a small fraction of the electromagnetic (EM) spectrum. In recent years radio frequency (RF) hardware has become more widely available, for…

Image and Video Processing · Electrical Eng. & Systems 2019-01-15 Manikanta Kotaru , Guy Satat , Ramesh Raskar , Sachin Katti

We introduce a phase imaging mechanism for scanning transmission electron microscopy that exploits the complementary intensity changes of transmitted disks at different scattering angles. For scanning transmission electron microscopy, this…

Instrumentation and Detectors · Physics 2023-07-06 Binbin Wang , David W. McComb

Hyperspectral pansharpening consists of fusing a high-resolution panchromatic band and a low-resolution hyperspectral image to obtain a new image with high resolution in both the spatial and spectral domains. These remote sensing products…

Computer Vision and Pattern Recognition · Computer Science 2024-12-30 Matteo Ciotola , Giuseppe Guarino , Gemine Vivone , Giovanni Poggi , Jocelyn Chanussot , Antonio Plaza , Giuseppe Scarpa

The detection of ultrafast optical and radio-frequency (RF) signals is crucial for applications ranging from high-speed communications to advanced sensing. However, conventional detectors are fundamentally constrained by their intrinsic…

Multiphoton microscopy (MPM) has gained enormous popularity over the years for its capacity to provide high resolution images from deep within scattering samples1. However, MPM is generally based on single-point laser-focus scanning, which…

Optics · Physics 2018-12-14 Devin R. Beaulieu , Ian G. Davison , Thomas G. Bifano , Jerome Mertz

We introduce a wide-field magneto-optical microscope to probe magnetization dynamics with femtosecond temporal and sub-micrometer spatial resolution. We carefully calibrate the non-linear dependency between the magnetization of the sample…

This paper proposes the design of a custom mirror-based light field camera adapter that is cheap, simple in construction, and accessible. Mirrors of different shape and orientation reflect the scene into an upwards-facing camera to create…

Robotics · Computer Science 2016-12-19 Dorian Tsai , Donald G. Dansereau , Steve Martin , Peter Corke

Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging…

Wide-field imaging is widely adopted due to its fast acquisition, cost-effectiveness and ease of use. Its extension to direct volumetric applications, however, is burdened by the trade-off between resolution and depth of field (DOF),…

Thick biological tissues give rise to not only the scattering of incoming light waves, but also aberrations of the remaining unscattered waves. Due to the inability of existing optical imaging methodologies to overcome both of these…

Multifocal microscopy (MFM) offers high-speed three-dimensional imaging through the simultaneous image capture from multiple focal planes. Conventional MFM systems use a fabricated grating in the emission path for a single emission…

Biological Physics · Physics 2019-07-31 M. Junaid Amin , Sabine Petry , Haw Yang , Joshua W. Shaevitz

Light field (LF) images containing information for multiple views have numerous applications, which can be severely affected by low-light imaging. Recent learning-based methods for low-light enhancement have some disadvantages, such as a…

Computer Vision and Pattern Recognition · Computer Science 2023-08-16 Shansi Zhang , Nan Meng , Edmund Y. Lam

The recent introduction of portable, low-field MRI (LF-MRI) into the clinical setting has the potential to transform neuroimaging. However, LF-MRI is limited by lower resolution and signal-to-noise ratio, leading to incomplete…

We present an overview of the performances of a plenoptic microscope which combines the high sensitivity of a laser optical feedback imaging setup , the high resolution of optical synthetic aperture and a shot noise limited signal to noise…

Optics · Physics 2015-06-19 W Glastre , O Hugon , O Jacquin , H Guillet de Chatellus , E Lacot

Biological and biomedical samples are routinely examined using focused two-photon (2P) fluorescence microscopy due to its intrinsic axial sectioning and reduced out-of-focus bleaching. However, 2P imaging often requires excitation…

This thesis centres on the development of multidimensional fluorescence imaging tools, with a particular emphasis on fluorescence lifetime imaging (FLIM) microscopy for application to biological research. The key aspects of this thesis are…

Instrumentation and Detectors · Physics 2017-08-14 Egidijus Auksorius

Super-resolution imaging using sub-diffraction field localization by micron sized transparent beads (microspheres) was recently demonstrated [1]. Practical applications in microscopy require control over the positioning of the microspheres.…

Optics · Physics 2013-12-25 Leonid A. Krivitsky , Jia Jun Wang , Zengbo Wang , Boris Lukiyanchuk
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