A multimodal laser-scanning nonlinear optical microscope with a rapid broadband Fourier-transform coherent Raman modality
Abstract
Nonlinear optical microscopy allows rapid high-resolution microscopy with image contrast generated from intrinsic properties of the sample. Established modalities such as multiphoton excited fluorescence and second/third-harmonic generation can be combined with other nonlinear techniques, such as coherent Raman spectroscopy which typically allow chemical imaging of a single resonant vibrational mode of a sample. Here, we utilize a single ultrafast laser source to obtain broadband coherent Raman spectra on a microscope, together with other nonlinear microscopy approaches on the same instrument. We demonstrate that the coherent Raman modality allows broadband measurement (>1000 cm-1), with high spectral resolution (<5 cm-1), with a rapid spectral acquisition rate (3-12 kHz). This enables Raman hyperspectral imaging of > kilo-pixel images at >11 frames per second.
Keywords
Cite
@article{arxiv.2005.14020,
title = {A multimodal laser-scanning nonlinear optical microscope with a rapid broadband Fourier-transform coherent Raman modality},
author = {Faris Sinjab and Kazuki Hashimoto and Venktata Ramaiah Badarla and Junko Omachi and Takuro Ideguchi},
journal= {arXiv preprint arXiv:2005.14020},
year = {2020}
}