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Three-dimensional X-ray histology techniques offer a non-invasive alternative to conventional 2D histology, enabling volumetric imaging of biological tissues without the need for physical sectioning or chemical staining. However, the…

Image and Video Processing · Electrical Eng. & Systems 2025-09-12 Sarah C. Irvine , Christian Lucas , Diana Krüger , Bianca Guedert , Julian Moosmann , Berit Zeller-Plumhoff

We report the initial demonstrations of the use of single crystals in indirect x-ray imaging for x-ray phase contrast imaging at the Washington University in St. Louis Computational Bioimaging Laboratory (CBL). Based on single Gaussian peak…

Accelerator Physics · Physics 2018-05-14 A. H. Lumpkin , A. B. Garson , M. A. Anastasio

Two-dimensional Talbot array illuminators (TAIs) were designed, fabricated, and evaluated for high-resolution high-contrast x-ray phase imaging of soft tissue at 10-20keV. The TAIs create intensity modulations with a high compression ratio…

Sensitive to scattering from unresolved sample structures, the dark-field channel in full-field X-ray imaging provides complementary information to that offered by conventional attenuation and phase-contrast methods. A range of experimental…

We propose a method for optical nano-imaging in which the structure of a three-dimensional inhomogeneous medium may be recovered from far-field power measurements. Neither phase control of the illuminating field nor phase measurements of…

Mesoscale and Nanoscale Physics · Physics 2017-02-02 Alexander A. Govyadinov , George Y. Panasyuk , John C. Schotland

Medical differential phase contrast x-ray imaging (DPCI) promises improved soft-tissue contrast at lower x-ray dose. The dose strongly depends on both the angular sensitivity and on the visibility of a grating-based Talbot-Lau…

Medical Physics · Physics 2016-03-23 Oliver Preusche

Phase-contrast and dark-field imaging are relatively new X-ray imaging modalities that provide additional information to conventional attenuation-based imaging. However, this new information comes at the price of a more complex acquisition…

The field of biomedical imaging has undergone a rapid growth in recent years, mostly due to the implementation of ad-hoc designed experimental setups, theoretical support methods and numerical reconstructions. Especially for biological…

Biological Physics · Physics 2017-06-30 Daniele Ancora

Established x-ray diffraction methods allow for high-resolution structure determination of crystals, crystallized protein structures or even single molecules. While these techniques rely on coherent scattering, incoherent processes like…

Holographic coherent X-ray imaging enables nanoscale imaging of biological cells and tissues, rendering both phase and absorption contrast, i.e. real and imaginary parts of the refractive index. Unlike the standard model, which assumes a…

Analysis of PDEs · Mathematics 2026-05-29 Thorsten Hohage , Milad Karimi , Björn Müller

Knowledge gained through X-ray crystallography fostered structural determination of materials and greatly facilitated the development of modern science and technology in the past century. Atomic details of sample structures is achievable by…

Optics · Physics 2016-09-14 Hong Yu , Ronghua Lu , Shensheng Han , Honglan Xie , Guohao Du , Tiqiao Xiao , Daming Zhu

Computational imaging modalities support a simplification of the active architectures required in an imaging system and these approaches have been validated across the electromagnetic spectrum. Recent implementations have utilized…

Classical Physics · Physics 2016-08-24 Thomas Fromenteze , Xiaojun Liu , Michael Boyarsky , Jonah Gollub , David R. Smith

Phase Contrast Imaging (PCI), Dark-Field (DF) and Directional Dark-Field (DDF) imaging are recent X-ray imaging modalities that have demonstrated their interest by providing access to information and contrasts different from those provided…

Image and Video Processing · Electrical Eng. & Systems 2023-04-25 Clara Magnin , Laurene Quenot , Sylvain Bohic , Dan Mihai Cenda , Manuel Fernández Martínez , Blandine Lantz , Bertrand Faure , Emmanuel Brun

Image simulation remains under-exploited for the most widely used biological phase microscopy methods, because of difficulties in simulating partially coherent illumination. We describe an open-source toolbox, microlith…

Optics · Physics 2014-05-14 Shalin B. Mehta , Rudolf Oldenbourg

We have performed soft-X-ray angle resolved photoemission for metallic V$_2$O$_3$. Combining a micro focus beam (40 x 65 ${\mu}$m$^2$) and micro positioning techniques with a long working distance microscope, we have succeeded in observing…

A resolution-enhanced dark-field microscope by structured light illumination is proposed to improve resolution and contrast. A set of phase-shifted fringes are projected to the sample plane at large angle to capture modulated dark-field…

Image and Video Processing · Electrical Eng. & Systems 2022-02-14 Shaobai Li , Bofan Song , Rongguang Liang

Currently, diagnostic medicine uses a multitude of tools ranging from ionising radiation to histology analysis. With advances in piezoelectric crystal technology, high-frequency ultrasound imaging has developed to achieve comparatively high…

Medical Physics · Physics 2022-01-13 Akhil Kallepalli , James Halls , David B. James , Mark A. Richardson

The measurement of the Si lattice parameter by x-ray interferometry assumes the use of strain-free crystals, which might not be true because of intrinsic stresses due to surface relaxation, reconstruction, and oxidation. We used x-ray…

Applied Physics · Physics 2022-02-17 Enrico Massa , Carlo Paolo Sasso , Matteo Fretto , Luca Martino , Giovanni Mana

Electron and x-ray microscopes allow one to image the entire, unlabeled structure of hydrated materials at a resolution well beyond what visible light microscopes can achieve. However, both approaches involve ionizing radiation, so that…

Applied Physics · Physics 2020-04-22 Ming Du , Chris Jacobsen
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