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Reflection phase imaging provides label-free, high-resolution characterization of biological samples, typically using interferometric-based techniques. Here, we investigate reflection phase microscopy from intensity-only measurements under…

Quantitative Methods · Quantitative Biology 2019-12-18 Alex Matlock , Anne Sentenac , Patrick C. Chaumet , Ji Yi , Lei Tian

A simple coherent-imaging method due to Paganin et al. is widely employed for phase-amplitude reconstruction of samples using a single paraxial x-ray propagation-based phase-contrast image. The method assumes that the sample-to-detector…

Direct orientation contrast imaging of zinc-blende III-V materials is studied using scanning electron microscopy. A quantitative approach is taken using a 3 {\mu}m thick orientation-patterned GaP grown on GaAs sample, studying the…

X-ray microtomography at synchrotron sources is fundamentally limited by the high radiation dose applied to the samples, which restricts investigations to non-native tissue states and thereby compromises the biological relevance of the…

We propose a novel data-driven approach for analyzing synchrotron Laue X-ray microdiffraction scans based on machine learning algorithms. The basic architecture and major components of the method are formulated mathematically. We…

Materials Science · Physics 2020-10-12 Yintao Song , Nobumichi Tamura , Chenbo Zhang , Mostafa Karami , Xian Chen

We outline some basics of imaging using both fully-coherent and partially-coherent X-ray beams, with an emphasis on phase-contrast imaging. We open with some of the basic notions of X-ray imaging, including the vacuum wave equations and the…

Image and Video Processing · Electrical Eng. & Systems 2021-11-16 David M. Paganin , Daniele Pelliccia

We demonstrate a novel approach to the reconstruction of scanning probe x-ray diffraction tomography data with anisotropic poly crystalline samples. The method involves reconstructing a voxel map containing an orientation distribution…

This paper describes the development of a novel medical Xray imaging system adapted to the needs and constraints of low and middle income countries. The developed system is based on an indirect conversion chain: a scintillator plate…

Most material discrimination in security inspections is based on dual-energy x-ray imaging, which enables the determination of a material's effective atomic number (Zeff) as well as electron density and its consequent classification as…

Materials Science · Physics 2023-08-16 A. Astolfo , I. G. Haig , D. Bate , A. Olivo , P. Modregger

X-ray phase-contrast imaging has become indispensable for visualizing samples with low absorption contrast. In this regard, speckle-based techniques have shown significant advantages in spatial resolution, phase sensitivity, and…

Image and Video Processing · Electrical Eng. & Systems 2022-01-20 Zhi Qiao , Xianbo Shi , Yudong Yao , Michael J. Wojcik , Luca Rebuffi , Mathew J. Cherukara , Lahsen Assoufid

We demonstrate the use of a phase retrieval technique for propagation-based phase contrast neutron imaging with a polychromatic beam. This enables imaging samples with low absorption contrast and/or improving the signal-to-noise ratio to…

Scanning probe microscopy is one of the most versatile windows into the nanoworld, providing imaging access to a variety of sample properties, depending on the probe employed. Tunneling probes map electronic properties of samples, magnetic…

Mesoscale and Nanoscale Physics · Physics 2015-01-09 Thomas Häberle , Dominik Schmid-Lorch , Friedemann Reinhard , Jörg Wrachtrup

Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that…

Biological Physics · Physics 2019-11-06 Jiaming Guo , Camille Artur , Jason L. Eriksen , David Mayerich

Crystal orientation and strain mapping of an individual curved and asymmetrical core-shell hetero-nanowire is performed based on transmission electron microscopy. It relies on a comprehensive analysis of scanning nanobeam electron…

Materials Science · Physics 2023-01-16 Serhii Kryvyi , Slawomir Kret , Piotr Wojnar

We propose a novel architecture with a remote phosphor based modular and compact light source in a non-contact dermoscope prototype for skin cancer screening. The spectrum and color temperature of the output light can easily and…

Medical Physics · Physics 2014-06-05 Chandrajit Basu , Sebastian Schlangen , Merve Meinhardt-Wollweber , Bernhard Roth

We demonstrate that powder diffraction data can be collected from sub-micron crystals of a mbrane protein with nearly two orders of magnitude more atoms than the molecules commonly used for powder diffraction. The crystals of photosystem-1…

X-ray imaging is a fast, precise and non-invasive method of imaging which, when combined with computed tomography, provides detailed 3D rendering of samples. Incorporating propagation-based phase contrast can vastly improve data quality for…

Biological tissues are complex structures composed of many elements which make light-based tissue diagnostics challenging. Over the past decades, Monte Carlo technique has been used as a fundamental and versatile approach toward modeling…

Optics · Physics 2024-05-17 Maryam Ghahremani

Significance: Histopathological analysis of tissues is an essential tool for grading, staging, diagnosing and resecting cancers and other malignancies. Current histopathological techniques require substantial sample processing prior to…

Medical Physics · Physics 2021-07-14 Benjamin Ecclestone , Deepak Dinakaran , Parsin Haji Reza

In this paper, a new phase-retrieval algorithm from an X-ray schlieren image is proposed. The schlieren method allows phase-contrast imaging with an objective lens and a knife-edge filter placed at the back focal plane of the objective.…