Related papers: Using fluorescent beads to emulate single fluropho…
We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive…
Quantitative fluorescence analysis is often used to derive chemical properties, including stoichiometries, of biomolecular complexes. One fundamental underlying assumption in the analysis of fluorescence data -- whether it be the…
Nanoscale localization of point emitters is critical to several methods in optical fluorescence microscopy, including single-molecule super-resolution imaging and tracking. While the precision of the localization procedure has been the…
The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…
In this work we experimentally demonstrate for the first time a recently proposed criterion adressed to detect nonclassical behavior in the fluorescence emission of ensembles of single-photon emitters. In particular, we apply the method to…
Single-shot volumetric fluorescence (SVF) imaging offers a significant advantage over traditional imaging methods that require scanning across multiple axial planes as it can capture biological processes with high temporal resolution. The…
We establish a method, called pbFFS for photobleaching Fluctuation Fluorescence Spectroscopy, which aims at characterizing molecules or particles labelled with a unknown distribution or fluorophores. Using photobleaching as a control…
Optical fibres are essential for many types of highly-multiplexed and precision spectroscopy. The success of the new generation of multifibre instruments under construction to investigate fundamental problems in cosmology, such as the…
We explore theoretically the electroluminescence of single molecules. We adopt a local-electrode framework that is appropriate for scanning tunneling microscopy (STM) experiments where electroluminescence originates from individual…
We employ heterodyne interferometry to investigate the effect of a single organic molecule on the phase of a propagating laser beam. We report on the first phase-contrast images of individual molecules and demonstrate a single-molecule…
In this study, we report initial demonstrations of the use of single crystals in indirect x-ray imaging with a benchtop implementation of propagation-based (PB) x-ray phase contrast imaging. Based on single Gaussian peak fits to the x-ray…
The ultimate goal of the "Pi of the Sky" apparatus is observation of optical flashes of astronomical origin and other light sources variable on short timescales. We search mainly for optical emission of Gamma Ray Bursts, but also for…
We show how the effects of azimuthal optical aberrations on singular light beams can result in an intensity modulation in the beam waist or focal point spread function (PSF) that is directly proportional to the amplitude of the applied…
A new concept of using focus-diverse point spread functions (PSFs) for modal wavefront sensing (WFS) is explored. This is based on relatively straightforward image moment analysis of measured PSFs, which differentiates it from other…
Single molecule localization microscopy is a recently developed superresolution imaging technique to visualize structural properties of single cells. The basic principle consists in chemically attaching fluorescent dyes to the molecules,…
We present both theoretical and experimental results on fluorescence of single defect centers in diamond nanocrystals embedded in a planar dielectric microcavity. From a theoretical point of view, we show that the overall fluorescence…
We experimentally investigate the fluorescence photon emission characteristics for single q-dots by using optical nanofibers. We demonstrate that single q-dots can be deposited along an optical nanofiber systematically and reproducibly with…
Important applications of single-particle tracking (SPT) aim at deciphering the diffusion properties of single fluorescent nanoparticles immersed in heterogeneous environments, such as multi-cellular biological tissues. To maximize the…
Investigations that probe defects one at a time offer a unique opportunity to observe properties and dynamics that are washed out of ensemble measurements. Here we present confocal fluorescence measurements of individual defects in Al-doped…
In this paper, we study a macroscopic system of electrically interacting metallic beads organized as a sequence along an annulus. A random mechanical shaking mimics the thermal excitation. We exhibit non Fickian diffusion (Single File…