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We present a novel learning-based method to build a differentiable computational model of a real fluorescence microscope. Our model can be used to calibrate a real optical setup directly from data samples and to engineer point spread…
Spatial Fluorescence Cross Correlation Spectroscopy is a rarely investigated version of Fluorescence Correlation Spectroscopy, in which the fluorescence signals from different observation volumes are cross-correlated. In the reported…
We measured images and Fourier images of fluorescence for 0.11- and 0.22-$\mu$m-diameter dye-doped polystyrene micro-sphere beads on a solid immersion lens, and experimentally verified strongly-angle-dependent fluorescence intensities due…
The combination of different imaging modalities into single imaging platforms has a strong potential in biomedical sciences since it permits the analysis of complementary properties of the target sample. Here, we report on an extremely…
Incoherently illuminated or luminescent objects give rise to a low-contrast speckle-like pattern when observed through a thin diffusive medium, as such a medium effectively convolves their shape with a speckle-like point spread function…
Single fluorescent molecules, behaving as ideal electric dipole emitters, are powerful nanoscopic probes of complex optical fields. Here, this property is exploited to precisely map the polarization and vectorial structure of tightly…
Uncertainty in the wide-angle Point Spread Function (PSF) at large angles (tens of arcseconds and beyond) is one of the dominant sources of error in a number of important quantities in observational astronomy. Examples include the stellar…
Exploring the use of single-mode fibers (SMFs) in high precision Doppler spectrometers has become increasingly attractive since the advent of diffraction-limited adaptive optics systems on large-aperture telescopes. Spectrometers fed with…
We experimentally compare the optical bandwidth of a conventional single-mode fiber (SMF) with 3 different photonic crystal fibers (PCF) all optimized for visible applications. The spectral attenuation, single-turn bend loss, and mode-field…
Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…
Optical fluorescence imaging is capable of measuring both the spatial and rotational dynamics of single molecules. However, unavoidable measurement noise will result in inaccurate estimates of rotational dynamics, causing a molecule to…
Fluorescence fluctuation spectroscopy is applied to study molecules, passing through a small observation volume, usually subjected to diffusive or convective motion in liquid phase. We suggest that such a technique could be used to measure…
Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is…
Point-spread function of the probe forming optics ($PSF_{optics} $) is reported for the first time in an uncorrected (without multipole correctors) scanning electron microscope (SEM). In an SEM, the electron probe information is lost as the…
Because the opacity of clouds in substellar mass object (SMO) atmospheres depends on the composition and distribution of particle sizes within the cloud, a credible cloud model is essential for accurately modeling SMO spectra and colors. We…
Single dye molecules at cryogenic temperatures display many spectroscopic phenomena known from free atoms and are thus promising candidates for fundamental quantum optical studies. However, the existing techniques for the detection of…
Confocal microscopy of fluorescent labeled particles has been used to study the dynamical and structural properties of colloidal and granular matter in real space. Localization algorithms allow for a fully automatized determination of the…
The combination of single photon emitters (quantum dots) and tailored metal nanoparticles with defined size and shape allows a detailed study of the interaction between light and matter. The enhanced optical near-field of the nanoparticles…
Nanodiamonds containing luminescent point defects are widely explored for applications in quantum bio-sensing such as nanoscale magnetometry, thermometry, and electrometry. A key challenge in the development of such applications is a large…
We demonstrate how optical nanofibers can be used to manipulate and probe single-atom fluorescence. We show that fluorescence photons from a very small number of atoms, average atom number of less than 0.1, around the nanofiber can readily…