Related papers: Super-resolution three-dimensional fluorescence an…
We demonstrate an electro-optic wide-field method to enable fluorescence lifetime microscopy (FLIM) with high throughput and single-molecule sensitivity. Resonantly driven Pockels cells are used to efficiently gate images at 39 MHz,…
Structured illumination microscopy (SIM) is an optical super-resolution technique that enables live-cell imaging beyond the diffraction limit. Reconstruction of SIM data is prone to artefacts, which becomes problematic when imaging highly…
Magnetic Resonance Imaging (MRI) is a powerful imaging technique widely used for visualizing structures within the human body and in other fields such as plant sciences. However, there is a demand to develop fast 3D-MRI reconstruction…
We show that structural information can be extracted from single molecule localization microscopy (SMLM) data. More precisely, we reinterpret SMLM data as the measures of a phaseless optical diffraction tomography system for which the…
A new approach to three-dimensional (3D) dosimetry based on optically-stimulated luminescence (OSL) is presented. By embedding OSL-active particles into a transparent silicone matrix (PDMS), the well-established dosimetric properties of an…
We present a superpixel method for full spatial phase and amplitude control of a light beam using a digital micromirror device (DMD) combined with a spatial filter. We combine square regions of nearby micromirrors into superpixels by low…
The detection of spatial or temporal variations in very thin samples has important applications in the biological sciences. For example, cellular membranes exhibit changes in lipid composition and order, which in turn modulate their…
Digital micromirror device (DMD) serves in a significant part of computational optical setups as a means of encoding an image by the desired pattern. The most prominent is its usage in the so-called single-pixel camera experiment. This…
We combine confocal imaging, microfluidics and image analysis to record 3D-images of cells in flow. This enables us to recover the full 3D representation of several hundred living cells per minute. Whereas 3D confocal imaging has thus far…
We present a technique for mapping the complete 3D spatial intensity profile of a laser beam from its fluorescence in an atomic vapour. We propagate shaped light through a rubidium vapour cell and record the resonant scattering from the…
3D fluorescence microscopy is essential for understanding fundamental life processes through long-term live-cell imaging. However, due to inherent issues in imaging principles, it faces significant challenges including spatially varying…
Diffusion MRI (dMRI) is a critical non-invasive technique to estimate fiber orientation distribution (FOD) for characterizing white matter integrity. Estimating FOD from single-shell low angular resolution dMRI (LAR-FOD) is limited by…
Optimizing the performance of organic solar cells (OSCs) hinges on a comprehensive understanding of their nanostructures, yet traditional characterization methods often fall short, delivering incomplete structural snapshots. We introduce…
Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…
Fluorescence imaging allows for non-invasively visualizing and measuring key physiological parameters like pH and dissolved oxygen. In our work, we created two ratiometric fluorescent microsensors designed for accurately tracking dissolved…
White Light Interferometry (WLI) is a precise optical tool for measuring the 3D topography of microstructures. However, conventional WLI cannot capture the natural color of a sample's surface, which is essential for many microscale research…
Super-resolution mapping of the 3D orientation of fluorophores reveals the alignment of biological structures where the fluorophores are tightly attached, and thus plays a vital role in studying the organization and dynamics of…
There is widespread interest in estimating the fluorescence properties of natural materials in an image. However, the separation between reflected and fluoresced components is difficult, because it is impossible to distinguish reflected and…
Structured illumination microscopy (SIM) achieves superresolution in fluorescence imaging through patterned illumination and computational image reconstruction, yet current methods require bulky, costly modulation optics and high-precision…
Three-dimensional elements, with refractive index distribution structured at sub-wavelength scale, provide an expansive optical design space that can be harnessed for demonstrating multi-functional free-space optical devices. Here we…