3D orientation super-resolution spatial-frequency-shift microscopy
Abstract
Super-resolution mapping of the 3D orientation of fluorophores reveals the alignment of biological structures where the fluorophores are tightly attached, and thus plays a vital role in studying the organization and dynamics of bio-complexes. However, current super-resolution imaging techniques are either limited to 2D orientation mapping or suffer from slow speed and the requirement of special labels in 3D orientation mapping. Here, we propose a novel polarized virtual spatial-frequency-shift effect to overcome these restrictions to achieve a universal 3D orientation super-resolution mapping capability. To demonstrate the mechanism, we simulate the imaging process and reconstruct the spatial-angular information for sparsely distributed dipoles with random 3D orientations and microfilament-like structures decorated with fluorophores oriented parallel to them. The 3D orientation distribution can be recovered with a doubled spatial resolution and an average angular precision of up to 2.39 degrees. The performance of the approach with noise has also been analyzed considering real implementation.
Cite
@article{arxiv.2401.09085,
title = {3D orientation super-resolution spatial-frequency-shift microscopy},
author = {Xiaowei Liu and Mingwei Tang and Ning Zhou and Chenlei Pang and Zhong Wen and Xu Liu and Qing Yang},
journal= {arXiv preprint arXiv:2401.09085},
year = {2024}
}
Comments
22 pages, 5 figures