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Three-dimensional particle tracking is an essential tool in studying dynamics under the microscope, namely, fluid dynamics in microfluidic devices, bacteria taxis, cellular trafficking. The 3d position can be determined using 2d imaging…

Computer Vision and Pattern Recognition · Computer Science 2015-09-08 Eldad Afik

Recently, chiral structured illumination microscopy has been proposed to image fluorescent chiral domains at sub-wavelength resolution. Chiral structured illumination microscopy is based on the combination of structured illumination…

Optics · Physics 2021-07-28 Shiang-Yu Huang , Ankit Kumar Singh , Jer-Shing Huang

Diffraction analysis in four dimensional scanning transmission electron microscopy now enables the mapping of local structures including symmetry, strain, and polarization of materials. However, measuring the distribution of these…

Materials Science · Physics 2024-06-19 Tim B. Eldred , Jacob G. Smith , Wenpei Gao

Optical diffraction tomography is an indispensable tool for studying objects in three-dimensions due to its ability to accurately reconstruct scattering objects. Until now this technique has been limited to coherent light because spatial…

The non-interference three-dimensional refractive index(RI) tomography has attracted extensive attention in the life science field for its simple system implementation and robust imaging performance. However, the complexity inherent in the…

Optics · Physics 2023-06-13 Delong Yang , Shaohui Zhang , Yao Hu , Qun Hao

Biological cell imaging has become one of the most crucial research interests due to its wide-ranging applications in biomedical and microbiology studies. However, three-dimensional (3D) imaging of biological cells remains critically…

We use a spatial light modulator in a diffraction tomographic system to assess the accuracy of different refractive index reconstruction algorithms. Optical phase conjugation principles through complex media, allows us to quantify the error…

In many laboratories, conventional bright-field transmission microscopes are available to study the structure and organization principles of fibrous tissue samples, but they usually provide only 2D information. To access the third…

Super-resolution fluorescence microscopy, with a resolution beyond the diffraction limit of light, has become an indispensable tool to directly visualize biological structures in living cells at a nanometer-scale resolution. Despite…

Computer Vision and Pattern Recognition · Computer Science 2018-09-05 Yu Li , Fan Xu , Fa Zhang , Pingyong Xu , Mingshu Zhang , Ming Fan , Lihua Li , Xin Gao , Renmin Han

Intensity correlation microscopy (ICM), which is prominently known through antibunching microscopy or super-resolution optical fluctuation imaging (SOFI), provides superresolution through a correlation analysis of antibunching of…

Optics · Physics 2018-10-08 Anton Classen , Joachim von Zanthier , Girish S. Agarwal

In recent time there has been an increasing amount of interest in developing novel techniques for the generation of complex vector light beams. Amongst these, digital holography stands out as one of the most flexible and versatile with…

Three-dimensional electron diffraction (3DED) is a powerful technique providing for crystal structure solutions of sub-micron sized crystals too small for structure determination via X-ray techniques. The entry requirement, however, of a…

This paper presents a novel dual-functional hybrid Reconfigurable Intelligent Surface (RIS) for simultaneous sensing and reconfigurable reflections. We design a novel hybrid unit cell featuring dual elements, which share the same phase…

One of the most common methods for reconstructing three-dimensional (3D) images of real objects is digital holography. This technique relies on the use of electro-optical devices that modify the phase or amplitude of light fields in a…

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang

We demonstrate a label-free, scan-free {\it intensity} diffraction tomography technique utilizing annular illumination (aIDT) to rapidly characterize large-volume 3D refractive index distributions in vitro. By optimally matching the…

Optics · Physics 2020-01-01 Jiaji Li , Alex Matlock , Yunzhe Li , Qian Chen , Chao Zuo , Lei Tian

We present a tomographic imaging technique, termed Deep Prior Diffraction Tomography (DP-DT), to reconstruct the 3D refractive index (RI) of thick biological samples at high resolution from a sequence of low-resolution images collected…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Kevin C. Zhou , Roarke Horstmeyer

We demonstrate a three-dimensional (3D) optical diffraction tomographic technique with optimal frequency combination (OFC-ODT) for the 3D quantitative phase imaging of unlabeled specimens. Three sets of through-focus intensity images are…

Optics · Physics 2018-03-06 Jiaji Li , Qian Chen Jiasong Sun , Jialin Zhang , Junyi Ding , Chao Zuo

We present a new formalism for understanding the optical properties of metasurfaces, optically thin composite diffractive devices. The proposed technique, Rigorous Diffraction Interface Theory (R-DIT), provides an analytical framework for…

Optics · Physics 2017-05-24 Christopher M. Roberts , Viktor A Podolskiy

We have developed an automatic method for segmenting fluorescence lifetime (FLT) imaging microscopy (FLIM) images of cells inspired by a multi-resolution community detection (MCD) based network segmentation method. The image processing…

Medical Physics · Physics 2014-01-06 Dandan Hu , Pinaki Sarder , Peter Ronhovde , Sandra Orthaus , Samuel Achilefu , Zohar Nussinov