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Increasing demand for understanding the vast heterogeneity of cellular phenotypes has driven the development of imaging flow cytometry (IFC), that combines features of flow cytometry with fluorescence and bright field microscopy. IFC…

Quantitative Methods · Quantitative Biology 2019-02-06 Yuanyuan Han , Rui Tang , Yi Gu , Alex Ce Zhang , Wei Cai , Violet Castor , Sung Hwan Cho , William Alaynick , Yu-Hwa Lo

Conventional three-dimensional (3D) imaging methods require multiple measurements of the sample in different orientation or scanning. When the sample is probed with coherent waves, a single two-dimensional (2D) intensity measurement is…

Image and Video Processing · Electrical Eng. & Systems 2022-01-25 Tatiana Latychevskaia

This paper introduces a novel method for detailed 3D shape reconstruction utilizing thermal polarization cues. Unlike state-of-the-art methods, the proposed approach is independent of illumination and material properties. In this paper, we…

Computer Vision and Pattern Recognition · Computer Science 2025-10-27 Takahiro Kushida , Kenichiro Tanaka

The growing complexity of spatial and structural information in 3D data makes data inspection and visualization a challenging task. We describe a method to create a planar embedding of 3D treelike structures using their skeleton…

Graphics · Computer Science 2022-02-23 Ping Hu , Saeed Boorboor , Joseph Marino , Arie E. Kaufman

Chronic wound monitoring and management require accurate and efficient wound measurement methods. This paper presents a fast, non-invasive 3D wound measurement algorithm based on RGB-D imaging. The method combines RGB-D odometry with…

Computer Vision and Pattern Recognition · Computer Science 2026-01-28 Lena Harkämper , Leo Lebrat , David Ahmedt-Aristizabal , Olivier Salvado , Mattias Heinrich , Rodrigo Santa Cruz

The possibility to obtain a three-dimensional representation of a single object with sub-$\mu$m resolution is crucial in many fields, from material science to clinical diagnostics. This is typically achieved through tomography, which…

We present a method for single-shot three-dimensional imaging through scattering media with a three-dimensional memory effect. In the proposed computational process, a captured speckle image is two-dimensionally correlated with different…

Image and Video Processing · Electrical Eng. & Systems 2019-08-13 Ryoichi Horisaki , Yuka Okamoto , Jun Tanida

While NeRF-based 3D-aware image generation methods enable viewpoint control, limitations still remain to be adopted to various 3D applications. Due to their view-dependent and light-entangled volume representation, the 3D geometry presents…

Computer Vision and Pattern Recognition · Computer Science 2022-03-15 Minsoo Lee , Chaeyeon Chung , Hojun Cho , Minjung Kim , Sanghun Jung , Jaegul Choo , Minhyuk Sung

We show that the symmetries of image formation by scattering enable graph-theoretic manifold-embedding techniques to extract structural and timing information from simulated and experimental snapshots at extremely low signal. The approach…

Computational Physics · Physics 2011-09-27 Peter Schwander , Chun Hong Yoon , Abbas Ourmazd , Dimitrios Giannakis

Many image processing operations involve the modification of the spatial frequency content of images. Here we demonstrate object-plane spatial frequency filtering utilizing the angular sensitivity of a commercial spectral bandstop filter.…

We present a method for differentiable rendering of 3D surfaces that supports both explicit and implicit representations, provides derivatives at occlusion boundaries, and is fast and simple to implement. The method first samples the…

Graphics · Computer Science 2021-08-12 Forrester Cole , Kyle Genova , Avneesh Sud , Daniel Vlasic , Zhoutong Zhang

Traditional fluorescence staining is phototoxic to live cells, slow, and expensive; thus, the subcellular structure prediction (SSP) from transmitted light (TL) images is emerging as a label-free, faster, low-cost alternative. However,…

Computer Vision and Pattern Recognition · Computer Science 2024-07-04 Jintu Zheng , Yi Ding , Qizhe Liu , Yi Cao , Ying Hu , Zenan Wang

Magnetic particle imaging (MPI) is a non-invasive, non-ionizing imaging technique for the visualization and quantification of magnetic nanoparticles (MNPs). The technique is especially suitable for cell imaging as it offers zero background…

Primary neuronal cultures have been widely used to study neuronal morphology, neurophysiology, neurodegenerative processes, and molecular mechanism of synaptic plasticity underlying learning and memory. Yet, the unique behavioral properties…

Image and Video Processing · Electrical Eng. & Systems 2020-08-04 Mikhail E. Kandel , Eunjae Kim , Young Jae Lee , Gregory Tracy , Hee Jung Chung , Gabriel Popescu

Comprehensive assessment of the various aspects of the brain's microstructure requires the use of complementary imaging techniques. This includes measuring the spatial distribution of cell bodies (cytoarchitecture) and nerve fibers…

Brillouin microscopy is an emerging optical elastography technique that can be used to assess mechanical properties of biological samples in a 3D, all-optical and hence non-contact fashion. However, the low cross-section of spontaneous…

Optics · Physics 2024-09-04 Carlo Bevilacqua , Robert Prevedel

A new focal-plane three-dimensional (3D) imaging method based on temporal ghost imaging is proposed and demonstrated. By exploiting the advantages of temporal ghost imaging, this method enables slow integrating cameras have an ability of 3D…

Image and Video Processing · Electrical Eng. & Systems 2018-05-18 Zunwang Bo , Wenlin Gong , Shensheng Han

Optical diffraction tomography (ODT) is a tomographic technique that can be used to measure the three-dimensional (3D) refractive index distribution within living cells without the requirement of any marker. In principle, ODT can be…

Quantitative Methods · Quantitative Biology 2018-01-03 Paul Müller , Mirjam Schürmann , Chii J. Chan , Jochen Guck

We show the possibility to periodically modulate refractive index in atomic medium in space or/and time while simultaneously keeping vanishing absorption/gain. Such modulation is based on periodic resonant enhancement of refractive index,…

Quantum Physics · Physics 2015-05-28 Chris O'Brien , Olga Kocharovskaya

Structured illumination has been widely used for optical sectioning and 3D surface recovery. In a typical implementation, multiple images under non-uniform pattern illumination are used to recover a single object section. Axial scanning of…

Computer Vision and Pattern Recognition · Computer Science 2019-04-26 Shaowei Jiang , Jun Liao , Zichao Bian , Pengming Song , Garrett Soler , Kazunori Hoshino , Guoan Zheng