3D-Imaging and Quantification of Magnetic Nanoparticle Uptake by Living Cells
Abstract
Magnetic particle imaging (MPI) is a non-invasive, non-ionizing imaging technique for the visualization and quantification of magnetic nanoparticles (MNPs). The technique is especially suitable for cell imaging as it offers zero background contribution from the surrounding tissue, high sensitivity, and good spatial and temporal resolutions. Previous studies have demonstrated that the dynamic magnetic behaviour of MNPs changes during cellular binding and internalization. In this study, we demonstrate how this information is encoded in the MPI imaging signal. Through MPI imaging we are able to discriminate between free and cell-bound MNPs in reconstructed images. This technique was used to image and quantify the changes that occur in-vitro when free MNPs come into contact with cells and undergo cellularuptake over time. The quantitative MPI results were verified by a phenanthroline assay. The results showed a mean relative difference of 23.8% for the quantification of cell-bound MNPs. The insights gained from such observations provide a new window into fundamental biological processes and associated pathological changes occurring at a cellular level. This technique could therefore offer new opportunities for the early diagnosis of inflammatory diseases.
Keywords
Cite
@article{arxiv.1912.01259,
title = {3D-Imaging and Quantification of Magnetic Nanoparticle Uptake by Living Cells},
author = {Hendrik Paysen and Norbert Loewa and Anke Stach and James Wells and Olaf Kosch and Shailey Twamley and Marcus R. Makowski and Tobias Schaeffter and Antje Ludwig and Frank Wiekhorst},
journal= {arXiv preprint arXiv:1912.01259},
year = {2020}
}
Comments
9 pages (+2 pages supplementary methods)