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Recent advances in optical technology have significantly enhanced the resolution of imaging of living cells, achieving nanometer-scale precision. However, the crowded three-dimensional environment within cells presents a challenge for…

Optics · Physics 2024-11-20 Vahid Nasirimarekani , Zuzana Ditte , Eberhard Bodenschatz

This work introduces a novel reinterpretation of structured illumination (SI) microscopy for coherent imaging that allows three-dimensional imaging of complex refractive index (RI). To do so, we show that coherent SI is mathematically…

Optics · Physics 2017-05-11 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

This article presents a method to perform diffraction tomography in a standard microscope that includes an LED array for illumination. After acquiring a sequence of intensity-only images of a thick sample, a ptychography-based…

Optics · Physics 2015-10-30 Roarke Horstmeyer , Changhuei Yang

We present an external interferometric setup that is able to simultaneously acquire three wavelengths of the same sample instance without scanning or multiple exposures. This setup projects onto the monochrome digital camera three off-axis…

Imaging mass spectrometry (IMS) is a powerful tool for untargeted, highly multiplexed molecular mapping of tissue in biomedical research. IMS offers a means of mapping the spatial distributions of molecular species in biological tissue with…

Computer Vision and Pattern Recognition · Computer Science 2025-08-05 Yijie Zhang , Luzhe Huang , Nir Pillar , Yuzhu Li , Lukasz G. Migas , Raf Van de Plas , Jeffrey M. Spraggins , Aydogan Ozcan

Synchrotron infrared hyperspectral microscopy is a label-free and non-invasive technique well suited for imaging of chemical events in situ. It can track the spatial and temporal distributions of molecules of interests in a specimen in its…

Biological Physics · Physics 2021-07-20 Hoi-Ying N. Holman , Wujun Zhao , Jennifer D. Nill , Liang Chen , Sankar Raju Narayanasamy , Tina Jeoh

A novel non-invasive microscopy technique for imaging and sizing of folded DNA molecules with the use of photovoltaic tweezers and phase-sensitive detection is elaborated and realized. This novel method is compared with the state-of-the-art…

Atomic Physics · Physics 2025-02-27 Lusine Tsarukyan , Anahit Badalyan , Rafael Drampyan

Modern Flash X-ray diffraction Imaging (FXI) acquires diffraction signals from single biomolecules at a high repetition rate from X-ray Free Electron Lasers (XFELs), easily obtaining millions of 2D diffraction patterns from a single…

Image and Video Processing · Electrical Eng. & Systems 2020-10-28 Jing Liu , Stefan Engblom , Carl Nettelblad

Fiber-based micro-endoscopes are a critically important tool for minimally-invasive deep-tissue imaging. However, the state-of-the-art micro-endoscopes cannot perform three-dimensional imaging through dynamically-bent fibers without the use…

Optics · Physics 2022-11-09 Noam Badt , Ori Katz

A method is proposed for high-resolution, three-dimensional reconstruction of internal structure of objects from planar transmission images. The described approach can be used with any form of radiation or matter waves, in principle,…

Optics · Physics 2022-08-24 T. E. Gureyev , H. M. Quiney , L. J. Allen

The elastodynamic response of finite 3D phononic structures is analyzed by means of comparing experimental findings obtained through a laser Doppler vibrometry-based methodology and theoretical computations performed with the…

Materials Science · Physics 2019-04-24 I. K. Tragazikis , D. A. Exarchos , P. T. Dalla , K. Dassios , T. E. Matikas , I. E. Psarobas

Diffraction microtomography in coherent light is foreseen as a promising technique to image transparent living samples in three dimensions without staining. Contrary to conventional microscopy with incoherent light, which gives…

Optics · Physics 2009-11-13 Stanislas Vertu , Jean-Jacques Delaunay , Olivier Haeberle

Imaging through diffusers presents a challenging problem with various digital image reconstruction solutions demonstrated to date using computers. We present a computer-free, all-optical image reconstruction method to see through random…

Volumetric biological imaging often involves compromising high temporal resolution at the expense of high spatial resolution when popular scanning methods are used to capture 3D information. We introduce an integrated experimental and image…

Optical diffraction tomography (ODT) is a powerful non-invasive 3D imaging technique, but its combination with broadband light sources is difficult. In this study, we introduce ultrabroadband ODT, covering over 150 nm of visible spectral…

Optical diffraction tomography (ODT) is a three-dimensional (3D) label-free imaging technique. The 3D refractive index distribution of a sample can be reconstructed from multiple two-dimensional optical field images via ODT. Herein, we…

Optics · Physics 2021-02-03 Chansuk Park , Kyeoreh Lee , Yoonseok Baek , Yongkeun Park

There is an increasing need for label free methods that could reveal intracellular structures and dynamics. In this context, we develop a new optical tomography method working in transmission - Full-field optical transmission tomography…

Optical diffraction tomography (ODT) reconstructs a samples volumetric refractive index (RI) to create high-contrast, quantitative 3D visualizations of biological samples. However, standard implementations of ODT use interferometric…

Image and Video Processing · Electrical Eng. & Systems 2024-12-13 Shwetadwip Chowdhury , Michael Chen , Regina Eckert , David Ren , Fan Wu , Nicole Repina , Laura Waller

We propose a novel framework to automatically learn to aggregate and transform photometric measurements from multiple unstructured views into spatially distinctive and view-invariant low-level features, which are subsequently fed to a…

Computer Vision and Pattern Recognition · Computer Science 2024-12-12 Xiang Feng , Kaizhang Kang , Fan Pei , Huakeng Ding , Jinjiang You , Ping Tan , Kun Zhou , Hongzhi Wu

Internal properties of a sample can be observed by medical imaging tools, such as ultrasound devices, magnetic resonance imaging (MRI) and optical coherence tomography (OCT) which are based on relying on changes in material density or…

Computer Vision and Pattern Recognition · Computer Science 2020-12-22 Mehrdad Shafiei Dizaji , Devin Harris
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