Ultra-broadband Optical Diffraction Tomography
Abstract
Optical diffraction tomography (ODT) is a powerful non-invasive 3D imaging technique, but its combination with broadband light sources is difficult. In this study, we introduce ultrabroadband ODT, covering over 150 nm of visible spectral bandwidth with a lateral spatial resolution of 150 nm. Our work addresses a critical experimental gap by enabling the measurement of broadband refractive index changes in 3D samples, a crucial information that is difficult to assess with existing methodologies. We present broadband, spectrally resolved ODT images of HeLa cells, obtained via pulse-shaping based Fourier transform spectroscopy. The spectral observations enabled by ultrabroadband ODT, combined with material-dependent refractive index responses, allow for precise three-dimensional identification of the nanoparticles within cellular structures. Our work represents a crucial step towards time and spectrally-resolved tomography of complex 3D structures with implications for life and materials science applications.
Cite
@article{arxiv.2401.07391,
title = {Ultra-broadband Optical Diffraction Tomography},
author = {Martin Hörmann and Franco V. A. Camargo and Niek F. van Hulst and Giulio Cerullo and Matz Liebel},
journal= {arXiv preprint arXiv:2401.07391},
year = {2024}
}