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Though structured illumination (SI) microscopy is a popular imaging technique conventionally associated with fluorescent super-resolution, recent works have suggested its applicability towards sub-diffraction coherent imaging with…

Quantitative Methods · Quantitative Biology 2024-12-13 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

Sub-diffraction resolution imaging has played a pivotal role in biological research by visualizing key, but previously unresolvable, sub-cellular structures. Unfortunately, applications of far-field sub-diffraction resolution are currently…

Biological Physics · Physics 2017-05-02 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

Optical diffraction tomography (ODT) provides three-dimensional refractive index (RI) tomograms of a transparent microscopic object. However, because of the finite numerical aperture of objective lenses, ODT has the limited access to…

Optics · Physics 2017-11-22 Moosung Lee , Seungwoo Shin , YongKeun Park

Optical diffraction tomography (ODT) reconstructs a samples volumetric refractive index (RI) to create high-contrast, quantitative 3D visualizations of biological samples. However, standard implementations of ODT use interferometric…

Image and Video Processing · Electrical Eng. & Systems 2024-12-13 Shwetadwip Chowdhury , Michael Chen , Regina Eckert , David Ren , Fan Wu , Nicole Repina , Laura Waller

We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM).…

Optics · Physics 2018-01-04 Seungwoo Shin , Doyeon Kim , Kyoohyun Kim , Yongkeun Park

Holographic Tomography (HT) or Optical Diffraction Tomography (ODT) provides slice-by-slice information about the refractive index (RI) of three-dimensional (3D) samples and is emerging as an important label-free imaging modality for Life…

Image and Video Processing · Electrical Eng. & Systems 2025-04-22 N Goyal , K Khare

Simultaneous imaging of fluorescence-labeled and label-free phase objects in the same sample provides distinct and complementary information. Most multimodal fluorescence-phase imaging operates in transmission mode, capturing fluorescence…

Optics · Physics 2024-08-20 Renzhi He , Yucheng Li , Junjie Chen , Yi Xue

We present a tomographic imaging technique, termed Deep Prior Diffraction Tomography (DP-DT), to reconstruct the 3D refractive index (RI) of thick biological samples at high resolution from a sequence of low-resolution images collected…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Kevin C. Zhou , Roarke Horstmeyer

We demonstrate a motion-free intensity diffraction tomography technique that enables direct inversion of 3D phase and absorption from intensity-only measurements for weakly scattering samples. We derive a novel linear forward model,…

Image and Video Processing · Electrical Eng. & Systems 2018-06-26 Ruilong Ling , Waleed Tahir , Hsing-Ying Lin , Hakho Lee , Lei Tian

Deconvolution phase microscopy enables high-contrast visualization of transparent samples through reconstructions of their transmitted phases or refractive indexes. Herein, we propose a method to extend 2D deconvolution phase microscopy to…

Optics · Physics 2022-04-20 Herve Hugonnet , Mahn Jae Lee , Yongkeun Park

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

We introduce a single-frame diffractive imaging method called randomized probe imaging (RPI). In RPI, a sample is illuminated by a structured probe field containing speckles smaller than the sample's typical feature size. Quantitative…

The refractive index (RI) of cells and tissues is crucial in pathophysiology as a noninvasive and quantitative imaging contrast. Although its measurements have been demonstrated using three-dimensional quantitative phase imaging methods,…

The non-interference three-dimensional refractive index(RI) tomography has attracted extensive attention in the life science field for its simple system implementation and robust imaging performance. However, the complexity inherent in the…

Optics · Physics 2023-06-13 Delong Yang , Shaohui Zhang , Yao Hu , Qun Hao

Here, we present a novel microscopic technique for measuring wavelength-dependent three-dimensional (3-D) distributions of the refractive indices (RIs) of microscopic samples in the visible wavelengths. Employing 3-D quantitative phase…

Optics · Physics 2016-04-20 JaeHwang Jung , Kyoohyun Kim , Jonghee Yoon , YongKeun Park

Optical microscopy has been employed to derive salient characteristics of an object in various fields, including cell biology, flow cytometry, biopsy, and neuroscience. In particular, measuring the phase of light scattered from an object…

Optics · Physics 2025-03-18 Jeonghun Oh , Herve Hugonnet , Weisun Park , YongKeun Park

Optical Diffraction Tomography (ODT) is a powerful non-invasive imaging technique widely used in biological and medical applications. While significant progress has been made in transmission configuration, reflection ODT remains challenging…

Optics · Physics 2026-05-14 Thomas Wasik , Victor Barolle , Alexandre Aubry , Josselin Garnier

Structured Illumination Microscopy (SIM) allows access to spatial information beyond the diffraction limit by folding high frequency components into the optical system's base-band. Using various algorithmic techniques, an image containing…

Optics · Physics 2024-10-16 Doron Shterman , Guy Bartal

Optical diffraction tomography (ODT) is an interferometric microscopy technique capable of measuring 3-D refractive index (RI) distribution of transparent samples. Multiple 2-D holograms of a sample illuminated with various angles are…

Refractive Index Tomography is the inverse problem of reconstructing the continuously-varying 3D refractive index in a scene using 2D projected image measurements. Although a purely refractive field is not directly visible, it bends light…

Computer Vision and Pattern Recognition · Computer Science 2023-12-06 Brandon Zhao , Aviad Levis , Liam Connor , Pratul P. Srinivasan , Katherine L. Bouman
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