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We proposed a novel approach to coherent imaging of dynamic samples. The inter-frame similarity of the sample's local structures is found to be a powerful constraint in phasing a sequence of diffraction patterns. We devised a new image…

Optics · Physics 2024-07-11 Pengju Sheng , Fucai Zhang

Coherent diffraction imaging (CDI) is a promising imaging technique revealing most of the information from diffraction measurements. An ideal CDI should reconstruct complex-valued object from a single-shot far-field diffraction without any…

Optics · Physics 2022-08-26 An-Dong Xiong , Xiao-Peng Jin , Xv-Ri Yao , Qing Zhao

We suggest a new multimodal imaging technique for quantitatively measuring the integral (thickness-average) refractive index of the nuclei of live biological cells in suspension. For this aim, we combined quantitative phase microscopy with…

Coherent diffraction imaging (CDI) allows the retrieval of the structure of an isolated object, such as a macromolecule, from its diffraction pattern. CDI requires the fulfilment of two conditions: the imaging radiation must be coherent and…

Optics · Physics 2018-11-16 Tatiana Latychevskaia , Hans-Werner Fink

Coherent diffractive imaging is a technique that recovers the sample image by numerically inverting its diffraction pattern. We propose a generalization of this method for the inversion of multi-wavelength data. Using this approach, we show…

Optics · Physics 2020-05-08 Erik Malm , Edwin Fohtung , Anders Mikkelsen

Structured illumination microscopy (SIM) enables live cell, super-resolution imaging at high speeds. SIM uses sophisticated optical systems to generate pre-determined excitation light patterns, and reconstruction algorithms to enhance the…

In coherent diffractive imaging (CDI) the resolution of the reconstructed object is limited by the numerical aperture of the experimental setup. We present here a theoretical and numerical study for achieving super-resolution by…

Data Analysis, Statistics and Probability · Physics 2016-09-20 Tatiana Latychevskaia , Yuriy Chushkin , Hans-Werner Fink

Cells alter the path of light, a fact that leads to well-known aberrations in single cell or tissue imaging. Optical diffraction tomography (ODT) measures the biophysical property that causes these aberrations, the refractive index (RI).…

Quantitative Methods · Quantitative Biology 2017-08-14 Mirjam Schürmann , Gheorghe Cojoc , Salvatore Girardo , Elke Ulbricht , Jochen Guck , Paul Müller

Optical diffraction tomography is an indispensable tool for studying objects in three-dimensions due to its ability to accurately reconstruct scattering objects. Until now this technique has been limited to coherent light because spatial…

Knowledge of optical properties, such as the refractive index (RI), of biological tissues is important in optical imaging, as they influence the distribution and propagation of light in tissue. To accurately study the response of cancerous…

Biological Physics · Physics 2019-04-10 Neelam Hari , Priyanka Patel , Jacqueline Ross , Kevin Hicks , Frédérique Vanholsbeeck

Structured illumination microscopy (SIM) provides images of fluorescent objects at an enhanced resolution greater than that of conventional epifluorescence wide-field microscopy. Initially demonstrated in 1999 to enhance the lateral…

Optics · Physics 2022-03-09 James D. Manton

Correlation plenoptic imaging (CPI) is emerging as a promising approach to light-field imaging (LFI), a technique enabling simultaneous measurement of light intensity distribution and propagation direction from a scene. LFI allows…

Optics · Physics 2024-06-21 Gianlorenzo Massaro

We report a novel extension to structured illumination (SI) microscopy that utilizes the Talbot self-imaging effect to generate a SI pattern on a sample with field-of-view (FOV) and resolution unconstrained by the numerical aperture (NA) of…

Optics · Physics 2018-01-12 Shwetadwip Chowdhury , Jeffrey Chen , Joseph A. Izatt

We present a new two-snapshot structured light illumination (SLI) reconstruction algorithm for fast image acquisition. The new algorithm, which only requires two mutually {\pi} phase-shifted raw structured images, is implemented on a…

Optics · Physics 2017-10-25 Yunlong Meng , Wei Lin , Chenglin Li , Shih-chi Chen

Complex-field imaging is indispensable for numerous applications at wavelengths from X-ray to THz, with amplitude describing transmittance (or reflectivity) and phase revealing intrinsic structure of the target object. Coherent diffraction…

Image and Video Processing · Electrical Eng. & Systems 2021-04-07 Meng Li , Liheng Bian , Guoan Zheng , Andrew Maiden , Yang Liu , Yiming Li , Qionghai Dai , Jun Zhang

Diffraction microtomography in coherent light is foreseen as a promising technique to image transparent living samples in three dimensions without staining. Contrary to conventional microscopy with incoherent light, which gives…

Optics · Physics 2009-11-13 Stanislas Vertu , Jean-Jacques Delaunay , Olivier Haeberle

New lensless diffractive X-ray technic for micro-scale imaging of biological tissue is based on quantitative phase retrieval schemes. By incorporating refraction, this method yields improved contrast compared to purely absorption-based…

Numerical Analysis · Mathematics 2018-03-28 Victor Palamodov

This paper presents a Multispectral imaging (MSI) approach that combines the use of a diffractive optical element, and a deep learning algorithm for spectral reconstruction. Traditional MSI techniques often face challenges such as high…

Image and Video Processing · Electrical Eng. & Systems 2023-10-06 Khen Cohen , Tuval Kay

For quantitative phase imaging (QPI) based on transport-of-intensity equation (TIE), partially coherent illumination provides speckle-free imaging, compatibility with brightfield microscopy, and transverse resolution beyond coherent…

Optics · Physics 2017-05-31 Chao Zuo , Jiasong Sun , Jiaji Li , Jialin Zhang , Anand Asundi , Qian Chen

Ptychography enables coherent diffractive imaging (CDI) of extended samples by raster scanning across the illuminating XUV/X-ray beam thereby generalizing the unique advantages of CDI techniques. Table-top realizations of this method are…