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Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…

Biological Physics · Physics 2007-08-27 James H. Rice

Researchers from a broad spectrum of scientific and engineering disciplines are increasingly using near-field infrared spectroscopic techniques to characterize materials nondestructively and with nanoscale spatial resolution. However,…

Optics · Physics 2023-03-21 Jonathan M. Larson , Hans A. Bechtel , Robert Kostecki

IR spectroscopy has been widely used for chemical identification and quantitative analysis of reactions occurring in a specific time and space domains by measuring an average signal of the entire system1. Achieving IR measurements with…

Advancements in digital imaging technologies have sparked increased interest in using multiplexed immunofluorescence (mIF) images to visualise and identify the interactions between specific immunophenotypes with the tumour microenvironment…

Image and Video Processing · Electrical Eng. & Systems 2024-07-01 Piumi Sandarenu , Julia Chen , Iveta Slapetova , Lois Browne , Peter H. Graham , Alexander Swarbrick , Ewan K. A. Millar , Yang Song , Erik Meijering

We proposed a refractive index (RI) sensing method with temperature compensation by using an optical frequency comb (OFC) sensing cavity employing a multimode-interference (MMI) fiber, namely, the MMI-OFC sensing cavity. The MMI-OFC sensing…

Optical transition radiation interferometry (OTRI) has been shown to be a very useful technique to measure the divergence of electron beams with energies in the range of 15-100 MeV. However, application of this method to low energy or very…

Accelerator Physics · Physics 2007-05-23 A. G. Shkvarunets , R. B. Fiorito , P. G. O'Shea

4D live fluorescence microscopy is often compromised by prolonged high intensity illumination which induces photobleaching and phototoxic effects that generate photo-induced artifacts and severely impair image continuity and detail…

Image and Video Processing · Electrical Eng. & Systems 2026-02-17 Cunmin Zhao , Ziyuan Luo , Guoye Guan , Zelin Li , Yiming Ma , Zhongying Zhao , Renjie Wan

Recently, significant progress has been made in the study of methods for 3D reconstruction from multiple images using implicit neural representations, exemplified by the neural radiance field (NeRF) method. Such methods, which are based on…

Computer Vision and Pattern Recognition · Computer Science 2024-04-19 Wooseok Kim , Taiki Fukiage , Takeshi Oishi

In the present work a new model of a refractive index (RI) sensor is exhibited. This is based on a dual core micro-structured optical fiber (MOF), where two holes are introduced at the core centers. In this way, the model enhances the…

Optics · Physics 2018-03-16 G. Tsigaridas , V. Karvouniaris , G. Chalkiadakis , P. Persephonis

IRIS (InfraRed Imaging Spectrograph) is a first light near-infrared diffraction limited imager and integral field spectrograph being designed for the future Thirty Meter Telescope (TMT). IRIS is optimized to perform astronomical studies…

Uncertainties of refractive and group index in dispersion measurement by spectrally resolved white light interferometry are deeply analyzed. First, the contribution to uncertainty of the different parameters affecting both indices is…

We present an estimate of the performance that will be achieved during on orbit operations of the JWST Mid Infrared Instrument, MIRI. The efficiency of the main imager and spectrometer systems in detecting photons from an astronomical…

Instrumentation and Methods for Astrophysics · Physics 2015-08-19 Alistair Glasse , G. H. Rieke , E. Bauwens , Macarena Garcıa-Marın , M. E. Ressler , Steffen Rost , T. V. Tikkanen , B. Vandenbussche , G. S. Wright

Atomic Force Microscopy - Infrared (AFM-IR) spectroscopy allows spectroscopic studies in the mid-infrared spectral region with a spatial resolution better than 50 nm. We show that the high spatial resolution can be used to perform…

Quantitative Methods · Quantitative Biology 2017-04-06 Luca Quaroni , Katarzyna Pogoda , Joanna-Wiltowska Zuber , Wojciech Kwiatek

Fluorescence Lifetime Imaging (FLI) is a critical molecular imaging modality that provides unique information about the tissue microenvironment, which is invaluable for biomedical applications. FLI operates by acquiring and analyzing photon…

Image and Video Processing · Electrical Eng. & Systems 2024-12-06 Ismail Erbas , Vikas Pandey , Navid Ibtehaj Nizam , Nanxue Yuan , Amit Verma , Margarida Barosso , Xavier Intes

Conventional bright-field (BF) cytology of thyroid fine-needle aspiration biopsy (FNAB) suffers from staining variability and limited subcellular contrast. Here, we present a refractive index-correlated pseudocoloring (RICP) framework that…

This study introduces a graphene-assisted terahertz metamaterial absorber operating at a resonance frequency of 8.436 THz. The unit cell incorporates a graphene-based split-ring resonator (SRR) configuration with additional auxiliary splits…

Patient-specific therapies require that cells be manufactured in multiple batches of small volumes, making it a challenge for conventional modes of quality control. The added complexity of inherent variability (even within batches)…

Biological Physics · Physics 2018-04-17 Derrick Yong , Ahmad Amirul Abdul Rahim , Jesslyn Ong , May Win Naing

Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it provides information about the emitting state only. Here, we present an…

Biological Physics · Physics 2023-10-27 Pavel Malý , Dita Strachotová , Aleš Holoubek , Petr Heřman

High-throughput screening using cell images is an efficient method for screening new candidates for pharmaceutical drugs. To complete the screening process, it is essential to have an efficient process for analyzing cell images. This paper…

Computer Vision and Pattern Recognition · Computer Science 2024-02-28 Mizuki Fukasawa , Tomokazu Fukuda , Takuya Akashi

Neural Radiance Field (NeRF) research has attracted significant attention recently, with 3D modelling, virtual/augmented reality, and visual effects driving its application. While current NeRF implementations can produce high quality visual…

Computer Vision and Pattern Recognition · Computer Science 2023-06-02 Adrian Azzarelli , Nantheera Anantrasirichai , David R Bull