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We demonstrate sub-wavelength sectioning on biological samples with a conventional confocal microscope. This optical sectioning is achieved by the phenomenon of supercritical angle fuorescence, wherein only a fluorophore next to the…
Molecular interactions are key to many chemical and biological processes like protein function. In many signaling processes they occur in sub-cellular areas displaying nanoscale organizations and involving molecular assemblies. The…
Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…
We present an overview of the design of IRIS, an infrared (0.85 - 2.5 micron) integral field spectrograph and imaging camera for the Thirty Meter Telescope (TMT). With extremely low wavefront error (<30 nm) and on-board wavefront sensors,…
Laser illumination of tissue results in a characteristic fluorescence emission spectrum whose features depend on the type of tissue, viz., healthy, adenoma or malignant. Hence measurement of the fluorescence is potentially a rapid and…
Sun-induced fluorescence (SIF) as a close remote sensing based proxy for photosynthesis is accepted as a useful measure to remotely monitor vegetation health and gross primary productivity. In this work we present the new retrieval method…
Near-field imaging experiments exist both in optics and microwaves with often different methods and theoretical supports. For millimeter waves or THz waves, techniques from both fields can be merged to identify materials at the micron scale…
We report on infrared (IR) spectro-microscopy of the electronic excitations in nanometer-thick accumulation layers in FET devices based on poly(3-hexylthiophene). IR data allows us to explore the charge injection landscape and uncovers the…
Optical diffraction tomography (ODT) reconstructs a samples volumetric refractive index (RI) to create high-contrast, quantitative 3D visualizations of biological samples. However, standard implementations of ODT use interferometric…
Long baseline diffraction-limited optical aperture synthesis technology by interferometry plays an important role in scientific study and practical application. In contrast to amplitude (phase) interferometry, intensity interferometry --…
The James Webb Space Telescope is orders of magnitude more sensitive than any other facility across the near to mid-infrared wavelengths. Many approved programs take advantage of its highly stable point spread function (PSF) to directly…
Immunofluorescent (IF) imaging is crucial for visualizing biomarker expressions, cell morphology and assessing the effects of drug treatments on sub-cellular components. IF imaging needs extra staining process and often requiring cell…
Digital holographic microscopy is a thriving imaging modality that attracted considerable research interest in quantitative biological cell imaging due to its ability to not only create excellent label-free contrast, but also supply…
Precise measurements of the geometrical thickness of a sample and its refractive index are important for materials science, engineering, and medical diagnosis. Among the possible non-contact evaluation methods, optical interferometric…
Diffraction of light beams from the phase steps due to the abrupt changes in the boundary of step leads to Fresnel fringes that their visibility and intensity profile depend on the change of the step height or light incident angle. The…
Grazing-Incidence X-ray fluorescence (GIXRF) analysis, which is closely related to total-reflection XRF, is a very powerful technique for the in-depth analysis of many types of technologically relevant samples, e.g. nanoparticle…
Significance: Diffuse in-vivo Flow Cytometry (DiFC) is an emerging fluorescence sensing method to non-invasively detect labeled circulating cells in-vivo. However, due to Signal-to-Noise Ratio (SNR) constraints largely attributed to…
In recent years, Neural Radiance Fields (NeRFs) have demonstrated significant potential in encoding highly-detailed 3D geometry and environmental appearance, positioning themselves as a promising alternative to traditional explicit…
Concentration gradients at the microscale play a central role in many physical, chemical, and biological systems, yet their quantitative visualization remains challenging due to the limited optical contrast associated with changes in…
Radio-frequency interference (RFI) is a major systematic limitation in radio astronomy, particularly for science cases requiring high sensitivity, such as 21 cm cosmology. Traditionally, RFI is dealt with by identifying its signature in the…