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Compared to imaging in the visible and near-infrared regions below 900 nm, imaging in the second near-infrared window (NIR-II, 1000-1700 nm) is a promising method for deep-tissue high-resolution optical imaging in vivo mainly due to the…
Advancement in mid-infrared (MIR) technology has led to promising biomedical applications of MIR spectroscopy, such as liquid biopsy or breath diagnosis. On the contrary, MIR microscopy has been rarely used for live biological samples in an…
Optical diffraction tomography (ODT) is an interferometric microscopy technique capable of measuring 3-D refractive index (RI) distribution of transparent samples. Multiple 2-D holograms of a sample illuminated with various angles are…
Detector requirements for far infrared astronomy generally result in devices which exhibit a few-moded response to incident radiation. The sensitivity and spatial form of the individual modes to which such a detector is sensitive can be…
Knowledge of optical properties, such as the refractive index (RI), of biological tissues is important in optical imaging, as they influence the distribution and propagation of light in tissue. To accurately study the response of cancerous…
The refractive index of single microparticles is derived from precise measurement and rigorous modeling of the stiffness of a laser trap. We demonstrate the method for particles of four different materials with diameters from 1.6 to 5.2…
Surface molecules, distributed in diverse patterns and clusters on cell membranes, influence vital functions of living cells. It is therefore important to understand their molecular surface organisation under different physiological and…
In this work, we present a technique to determine the mid-infrared refractive indices of thin superconducting films using Fourier transform infrared spectroscopy (FTIR). In particular, we performed FTIR transmission and reflection…
Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…
A non-invasive, in-situ calibration method for Total Internal Reflection Microscopy (TIRM) based on optical tweezing is presented which greatly expands the capabilities of this technique. We show that by making only simple modifications to…
The Total Internal Reflection Ultrasonic Sensor (TIRUS) is a device especially aimed at detecting subsurface flaws in a tested specimen based on the frustrated total internal reflection of bulk ultrasonic waves in the sensor body [1]. In…
Mid-infrared photo-induced force microscopy (IR-PiFM/PiF-IR) enables high-resolution chemical imaging of surfaces with lateral resolution less than 5 nm. Here are some answers to questions about the physical background, practical handling…
White light spectral interferometry is applied to measure the refractive index in absorbing liquids in the spectral range from 400 to 1000 nm. We analyze the influence of absorption on the visibility of interferometric fringes and,…
Multiplexed immuno-fluorescence tissue imaging, allowing simultaneous detection of molecular properties of cells, is an essential tool for characterizing the complex cellular mechanisms in translational research and clinical practice. New…
Three-dimensional molecular imaging of living cells is essential for unraveling cellular metabolism and response to therapies. However, existing volumetric methods, including fluorescence microscopy and quantitative phase imaging, either…
In a recent study entitled "Cell nuclei have lower refractive index and mass density than cytoplasm", we provided strong evidence indicating that the nuclear refractive index (RI) is lower than the RI of the cytoplasm for several cell…
Magnetic resonance imaging (MRI) is the cornerstone technique for diagnostic medicine, biology, and neuroscience. This imaging method is highly innovative, noninvasive and its impact continues to grow. It can be used for measuring changes…
Several types of Wavefront Sensors (WFS) are nowadays available in the field of Adaptive Optics (AO). Generally speaking, their basic principle consists in measuring slopes or curvatures of Wavefront Errors (WFE) transmitted by a telescope,…
Phase-sensitive integrated photonic devices are highly susceptible to minor manufacturing deviations, resulting in significant performance inconsistencies. This variability has limited the scalability and widespread adoption of these…
We present an overview of the design of IRIS, an infrared (0.84 - 2.4 micron) integral field spectrograph and imaging camera for the Thirty Meter Telescope (TMT). With extremely low wavefront error (<30 nm) and on-board wavefront sensors,…