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Reconfigurable photonic circuits have applications ranging from next-generation computer architectures to quantum networks, coherent radar and optical metamaterials. However, complete reconfigurability is only currently practical on…

We consider the use of digital signal processing to reconstruct continuous reflectance spectra using a small finite set of randomly illuminated light emitting diodes (LEDs). We simulate the use of LEDs having identical spectral distance and…

Optics · Physics 2025-10-29 John C. Howell , Pieter H. Neethling , Tjaart P. J. Kruger

Fluorescence microscopy is essential in biological and medical research, providing critical insights into cellular structures. However, limited by optical diffraction and background noise, a substantial amount of hidden information is still…

Biological Physics · Physics 2026-04-14 Xiaofeng Zhang , Yongsheng Huang , Jielong Yang , Zhili Wang , Si Chen , Linbo Liu , Xin Ge

Stroboscopic nanoscale imaging with free electron laser light is revolutionizing our understanding of fast dynamics in heterogeneous systems. The short wavelength of X-ray and extreme ultraviolet radiation makes it possible to achieve…

Neuromorphic Computing implemented in photonic hardware is one of the most promising routes towards achieving machine learning processing at the picosecond scale, with minimum power consumption. In this work, we present a new concept for…

Emerging Technologies · Computer Science 2022-11-01 K. Sozos , A. Bogris , P. Bienstman , G. Sarantoglou , S. Deligiannidis , C. Mesaritakis

We propose a 3D super-resolution approach to improve both lateral and axial spatial resolution in Total Internal Reflectance Fluorescence (TIRF) imaging applications. Our approach, called 3D-COL0RME (3D - Covariance-based $\ell_0$…

Medical Physics · Physics 2022-07-12 Vasiliki Stergiopoulou , Luca Calatroni , Sébastien Schaub , Laure Blanc-Féraud

The spectral resolution of a dispersive astronomical spectrograph is limited by the trade-off between throughput and the width of the entrance slit. Photonic guided-wave transitions have been proposed as a route to bypass this trade-off, by…

Evanescent light excitation is widely used in super-resolution fluorescence microscopy to confine light and reduce background noise. Herein we propose a method of exploiting evanescent light in the context of emission. When a fluorophore is…

Biological Physics · Physics 2015-09-30 N. Bourg , C. Mayet , G. Dupuis , T. Barroca , P. Bon , S. Lécart , E. Fort , S. Lévêque-Fort

Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…

Image and Video Processing · Electrical Eng. & Systems 2022-01-19 Varun Mannam , Xiaotong Yuan , Scott Howard

Panchromatic (PAN) and multi-spectral (MS) image fusion, named Pan-sharpening, refers to super-resolve the low-resolution (LR) multi-spectral (MS) images in the spatial domain to generate the expected high-resolution (HR) MS images,…

Computer Vision and Pattern Recognition · Computer Science 2022-10-18 Keyu Yan , Man Zhou , Jie Huang , Feng Zhao , Chengjun Xie , Chongyi Li , Danfeng Hong

A unified method for three-dimensional reconstruction of objects from transmission images collected at multiple illumination directions is described. The method may be applicable to experimental conditions relevant to absorption-based,…

Medical Physics · Physics 2022-12-07 Timur E. Gureyev , Hamish G. Brown , Harry M. Quiney , Leslie J. Allen

Live cell culture is crucial in biomedical studies for analyzing cell properties and dynamics in vitro. This study focuses on segmenting unstained live cells imaged with bright-field microscopy. While many segmentation approaches exist for…

Quantitative Methods · Quantitative Biology 2025-08-26 Surajit Das , Gourav Roy , Pavel Zun

Fourier Ptychographic Microscopy (FPM) is a computational imaging method that is able to super-resolve features beyond the diffraction-limit set by the objective lens of a traditional microscope. This is accomplished by using synthetic…

Signal Processing · Electrical Eng. & Systems 2019-04-09 Michael Kellman , Emrah Bostan , Michael Chen , Laura Waller

A lensfree spectral light-field fusion microscopy (LSLFM) system is presented for enabling contrast- and resolution-enhanced imaging of biological specimens. LSLFM consists of a pulsed multispectral lensfree microscope for capturing…

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

Biological Physics · Physics 2024-06-19 Mohamadreza Fazel , Michael J. Wester

On-chip nanophotonics serves as the foundation for the new generation of information technology, but it is challenged by the diffraction limit of light. With the capabilities of confining light into (deep) subwavelength volumes, plasmonics…

Fluorescence lifetime imaging microscopy (FLIM) systems are limited by their slow processing speed, low signal-to-noise ratio (SNR), and expensive and challenging hardware setups. In this work, we demonstrate applying a denoising…

Image and Video Processing · Electrical Eng. & Systems 2021-06-09 Varun Mannam , Yide Zhang , Xiaotong Yuan , Takashi Hato , Pierre C. Dagher , Evan L. Nichols , Cody J. Smith , Kenneth W. Dunn , Scott Howard

Point-scanning microscopy approaches are transforming super-resolution imaging. Despite achieving parallel high-speed imaging using multifocal techniques, efficient multi-color capability with high-quality illumination is currently lacking.…

Optics · Physics 2023-03-14 Ning Xu , Sarah E. Bohndiek , Zexing Li , Cilong Zhang , Qiaofeng Tan

The characterization of nanostructured surfaces with sensitivity in the sub-nm range is of high importance for the development of current and next generation integrated electronic circuits. Modern transistor architectures for e.g. FinFETs…

Interferometric-based microscopies stand as powerful label-free approaches for monitoring and characterising chemical reactions and heterogeneous nanoparticle systems in real time with single particle sensitivity. Nevertheless, coherent…

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