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Fluorescence microscopy is widely used for the study of biological specimens. Deconvolution can significantly improve the resolution and contrast of images produced using fluorescence microscopy; in particular, Bayesian-based methods have…

Methodology · Statistics 2015-02-04 Alexander Wong , Xiao Yu Wang , Maud Gorbet

In some super-resolution techniques, adjacent points are illuminated at different times. Thereby, their locations and light intensities can be detected even if the images are very blurred due to diffraction. According to conventional…

Image and Video Processing · Electrical Eng. & Systems 2019-12-10 Edward Y. Sheffield

Existing single-image denoising algorithms often struggle to restore details when dealing with complex noisy images. The introduction of near-infrared (NIR) images offers new possibilities for RGB image denoising. However, due to the…

Computer Vision and Pattern Recognition · Computer Science 2025-03-25 Yuchen Wang , Hongyuan Wang , Lizhi Wang , Xin Wang , Lin Zhu , Wanxuan Lu , Hua Huang

Single particle-resolved fluorescence imaging is an enabling technology in cold-atom physics. However, so far, this technique was not available for nanophotonic atom-light interfaces. Here, we image single atoms that are trapped and…

High-numerical-aperture (NA) oblique plane microscopy enables noninvasive fluorescence imaging of subcellular dynamics without requiring radical sample modification. However, performance degrades at depth in multicellular specimens as…

Fourier light-field microscopy (FLFM) uses a micro-lens array (MLA) to segment the Fourier Plane of the microscopic objective lens to generate multiple two-dimensional perspective views, thereby reconstructing the three-dimensional(3D)…

Optics · Physics 2022-08-29 Haixin Huang , Haoyuan Qiu , Hanzhe Wu , Yihong Ji , Heng Li , Bin Yu , Danni Chen , Junle Qu

Highly ordered periodic arrays of silver nanoparticles have been fabricated which exhibit surface plasmon resonances in the visible spectrum. We demonstrate the ability of these structures to alter the fluorescence properties of vicinal dye…

Other Condensed Matter · Physics 2009-11-13 T. Ritman-Meer , N. I. Cade , D. Richards

Fast scanning probe microscopy enabled via machine learning allows for a broad range of nanoscale, temporally resolved physics to be uncovered. However, such examples for functional imaging are few in number. Here, using piezoresponse force…

Digital imaging has been steadily improving over the past decades and we are moving towards a wide use of multi- and hyperspectral cameras. A key component of such imaging systems are color filter arrays, which define the spectrum of light…

Coordinate networks like Multiplicative Filter Networks (MFNs) and BACON offer some control over the frequency spectrum used to represent continuous signals such as images or 3D volumes. Yet, they are not readily applicable to problems for…

Computer Vision and Pattern Recognition · Computer Science 2022-10-28 Shayan Shekarforoush , David B. Lindell , David J. Fleet , Marcus A. Brubaker

Recently, imaging by microspheres and dielectric particle-lenses emerged as a simple solution to obtaining super-resolution images of nanoscale devices and structures. Calibrated resolution of ~{\lambda}/6 - {\lambda}/8 has been…

Optics · Physics 2021-08-26 Zengbo Wang , Boris Luk'yanchuk

Conventional super-resolution methods suffer from two drawbacks: substantial computational cost in upscaling an entire large image, and the introduction of extraneous or potentially detrimental information for downstream computer vision…

Computer Vision and Pattern Recognition · Computer Science 2023-12-12 Tianyi Zhang , Kishore Kasichainula , Yaoxin Zhuo , Baoxin Li , Jae-sun Seo , Yu Cao

Fluorescence microscopy, while being a key driver for progress in the life sciences, is also subject to technical limitations. To overcome them, computational multiplexing techniques have recently been proposed, which allow multiple…

Computer Vision and Pattern Recognition · Computer Science 2025-10-22 Ashesh Ashesh , Florian Jug

Modern high-resolution microscopes, such as the scanning tunneling microscope, are commonly used to study specimens that have dense and aperiodic spatial structure. Extracting meaningful information from images obtained from such…

Computational Physics · Physics 2018-08-28 Sky C. Cheung , John Y. Shin , Yenson Lau , Zhengyu Chen , Ju Sun , Yuqian Zhang , John N. Wright , Abhay N. Pasupathy

Super-resolution optical fluctuation imaging (SOFI) provides a resolution beyond the diffraction limit by analysing stochastic fluorescence fluctuations with higher-order statistics. Using nth order spatio-temporal cross-cumulants the…

Single particle imaging (SPI) at X-ray free electron lasers (XFELs) is particularly well suited to determine the 3D structure of particles in their native environment. For a successful reconstruction, diffraction patterns originating from a…

Data Analysis, Statistics and Probability · Physics 2021-12-17 Dameli Assalauova , Alexandr Ignatenko , Fabian Isensee , Sergey Bobkov , Darya Trofimova , Ivan A. Vartanyants

Achromatic combinations of a diffractive Phase Fresnel Lens and a refractive correcting element have been proposed for X-ray and gamma-ray astronomy and for microlithography, but considerations of absorption often dictate that the…

Astrophysics · Physics 2009-11-10 Gerald K. Skinner

Application of circularly polarized beams in interferometric illumination of colloid sphere monolayers enables the direct fabrication of rectangular patterns consisting of circular nanohole miniarrays in metal films. The spectral and…

Optics · Physics 2020-05-18 Emese Toth , Aron Sipos , Oliver A. Fekete , Maria Csete

A miniaturized full-frequency encoded illumination (mini-FEI) chip is presented for high-throughput super-resolution imaging using the spatial frequency shift (SFS) effect. A tunable full SFS scheme is achieved through propagating and…

Single particle cryo-electron microscopy has become a critical tool in structural biology over the last decade, able to achieve atomic scale resolution in three dimensional models from hundreds of thousands of (noisy) two-dimensional…

Numerical Analysis · Mathematics 2023-07-19 Aaditya V. Rangan , Leslie Greengard
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