Mechanical-scan-free and multi-color super-resolution imaging with diffractive spot array illumination
Abstract
Point-scanning microscopy approaches are transforming super-resolution imaging. Despite achieving parallel high-speed imaging using multifocal techniques, efficient multi-color capability with high-quality illumination is currently lacking. In this paper, we present for the first time Mechanical-scan-free and multi-Color Super-resolution Microscopy (MCoSM) by spot array illumination, which enables mechanical-scan-free super-resolution imaging with adjustable resolution and field of view (FoV) based on spatial light modulators (SLMs). Through 100s-10,000s super-resolution spot illumination with different FoV for imaging, we demonstrate the adjustable capacity of MCoSM. MCoSM extends current spectral imaging capabilities through a time-sharing process of different color illumination with phase-shifting scanning, while retaining the spatial flexibility of super-resolution imaging with diffractive spot array illumination. To showcase the prospects for further combining MCoSM with multi-color imaging, we also perform spectral unmixing (four-colors) on images of fluorescent beads at high resolution. MCoSM provides a versatile platform for studying molecular interactions in complex samples at the nanoscale level.
Cite
@article{arxiv.2303.06988,
title = {Mechanical-scan-free and multi-color super-resolution imaging with diffractive spot array illumination},
author = {Ning Xu and Sarah E. Bohndiek and Zexing Li and Cilong Zhang and Qiaofeng Tan},
journal= {arXiv preprint arXiv:2303.06988},
year = {2023}
}
Comments
21 pages, 5 figures