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Fluorescence molecular tomography (FMT) is an emerging powerful tool for biomedical research. There are two factors that influence FMT reconstruction most effectively. The first one is the regularization techniques. Traditional methods such…

Image and Video Processing · Electrical Eng. & Systems 2021-09-09 Yan Liu , Wuwei Ren , Habib Ammari

Optical mapping by direct visualization of individual DNA molecules, stretched in nanochannels with sequence-specific fluorescent labeling, represents a promising tool for disease diagnostics and genomics. An important challenge for this…

Quantitative Methods · Quantitative Biology 2015-01-21 Charleston Noble , Adam N. Nilsson , Camilla Freitag , Jason P. Beech , Jonas O. Tegenfeldt , Tobias Ambjörnsson

Lensless on-chip microscopy has shown great potential for biomedical imaging due to its large-area and high-throughput imaging capabilities. By combining the pixel super-resolution (PSR) technique, it can improve the resolution beyond the…

Image and Video Processing · Electrical Eng. & Systems 2024-09-06 Xuyang Chang , Shaowei Jiang , Yongcun Hu , Liheng Bian

Structured illumination microscopy (SIM) achieves superresolution in fluorescence imaging through patterned illumination and computational image reconstruction, yet current methods require bulky, costly modulation optics and high-precision…

Optics · Physics 2024-04-15 Tamal Roy , Peter T. Brown , Douglas P. Shepherd , Lisa V. Poulikakos

Disordered metasurfaces offer unique properties unattainable with periodic or ordered metasurfaces, notably the absence of deterministic interference effects at specific wavelengths and angles. In this work, we introduce a lithography-free…

Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…

Three-dimensional fluorescence microscopy often suffers from anisotropy, where the resolution along the axial direction is lower than that within the lateral imaging plane. We address this issue by presenting Dual-Cycle, a new framework for…

Image and Video Processing · Electrical Eng. & Systems 2022-09-26 Tomas Kerepecky , Jiaming Liu , Xue Wen Ng , David W. Piston , Ulugbek S. Kamilov

We consider the problem of active 3D imaging using single-shot structured light systems, which are widely employed in commercial 3D sensing devices such as Apple Face ID and Intel RealSense. Traditional structured light methods typically…

Computer Vision and Pattern Recognition · Computer Science 2025-12-17 Jiaheng Li , Qiyu Dai , Lihan Li , Praneeth Chakravarthula , He Sun , Baoquan Chen , Wenzheng Chen

Parallelism patterns (e.g., map or reduce) have proven to be effective tools for parallelizing high-performance applications. In this paper, we study the recursive registration of a series of electron microscopy images - a time consuming…

Distributed, Parallel, and Cluster Computing · Computer Science 2020-10-26 Marcin Copik , Tobias Grosser , Torsten Hoefler , Paolo Bientinesi , Benjamin Berkels

Metasurfaces play a key role in functionalizing light at the nanoscale. Existing dielectric metasurfaces, however, are often limited to geometric primitives and their usage in emergent hybrid metasurfaces is hampered as confinement of light…

Resolution level and reconstruction quality in nano-computed tomography (nano-CT) are in part limited by the stability of microscopes, because the magnitude of mechanical vibrations during scanning becomes comparable to the imaging…

Computational Engineering, Finance, and Science · Computer Science 2021-03-02 Viktor Nikitin , Vincent De Andrade , Azat Slyamov , Benjamin J. Gould , Yuepeng Zhang , Vandana Sampathkumar , Narayanan Kasthuri , Doga Gursoy , Francesco De Carlo

Hyperspectral cameras face challenging spatial-spectral resolution trade-offs and are more affected by shot noise than RGB photos taken over the same total exposure time. Here, we present a colorization algorithm to reconstruct…

Computer Vision and Pattern Recognition · Computer Science 2024-03-19 M. Kerem Aydin , Qi Guo , Emma Alexander

Linear super-resolution microscopy via synthesis aperture approach permits fast acquisition because of its wide-field implementations, however, it has been limited in resolution because a missing spatial-frequency band occurs when trying to…

Highly confined vectorial electromagnetic field distributions represent an excellent tool for detailed studies in nano-optics and high resolution microscopy, such as nonlinear microscopy, advanced fluorescence imaging or nanoplasmonics.…

Optics · Physics 2014-07-03 Thomas Bauer , Sergej Orlov , Ulf Peschel , Peter Banzer , Gerd Leuchs

Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and…

Lensfree on-chip microscopy is an emerging imaging technique that can be used to visualize and study biological specimens without the need for imaging lens systems. Important issues that can limit the performance of lensfree on-chip…

Optics · Physics 2015-05-11 Alexander Wong , Farnoud Kazemzadeh , Chao Jin , Xiao Yu Wang

When taking photos in dim-light environments, due to the small amount of light entering, the shot images are usually extremely dark, with a great deal of noise, and the color cannot reflect real-world color. Under this condition, the…

Computer Vision and Pattern Recognition · Computer Science 2019-04-17 Di Zhao , Lan Ma , Songnan Li , Dahai Yu

In coherent X-ray diffraction microscopy the diffraction pattern generated by a sample illuminated with coherent x-rays is recorded, and a computer algorithm recovers the unmeasured phases to synthesize an image. By avoiding the use of a…

Camera arrays provide spatial and angular information within a single snapshot. With refocusing methods, focal planes can be altered after exposure. In this letter, we propose a light field refocusing method to improve the imaging quality…

Image and Video Processing · Electrical Eng. & Systems 2021-08-29 Yingqian Wang , Jungang Yang , Yulan Guo , Chao Xiao , Wei An

We present a structured-illumination technique for full-field super-resolution transmission X-ray microscopy, which employs Fourier spectral decomposition inspired by established methods in visible-light microscopy. A 2D grating creating…