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The resolution of X-ray diffraction microscopy is limited by the maximum dose that can be delivered prior to sample damage. In the proposed Serial Crystallography method, the damage problem is addressed by distributing the total dose over…

Electron and x-ray microscopes allow one to image the entire, unlabeled structure of hydrated materials at a resolution well beyond what visible light microscopes can achieve. However, both approaches involve ionizing radiation, so that…

应用物理 · 物理学 2020-04-22 Ming Du , Chris Jacobsen

Following the recent developement of Fourier ptychographic microscopy (FPM) in the visible range by Zheng et al. (2013), we propose an adaptation for hard x-rays. FPM employs ptychographic reconstruction to merge a series of low-resolution,…

仪器与探测器 · 物理学 2016-09-27 H. Simons , H. F. Poulsen , J. P. Guigay , C. Detlefs

Advances in microscopy imaging enable researchers to visualize structures at the nanoscale level thereby unraveling intricate details of biological organization. However, challenges such as image noise, photobleaching of fluorophores, and…

图像与视频处理 · 电气工程与系统科学 2024-09-19 Pamela Osuna-Vargas , Maren H. Wehrheim , Lucas Zinz , Johanna Rahm , Ashwin Balakrishnan , Alexandra Kaminer , Mike Heilemann , Matthias Kaschube

In-situ X-ray diffraction (XRD) and transmission electron microscopy (TEM) have been used to investigate many physical science phenomena, ranging from phase transitions, chemical reaction and crystal growth to grain boundary dynamics. A…

The challenge of imaging low-density objects in an electron microscope without causing beam damage is significant in modern TEM. This is especially true for life science imaging, where the sample, rather than the instrument, still…

光学 · 物理学 2025-01-29 Francisco Vega Ibáñez , Jo Verbeeck

Coherent X-ray diffraction microscopy is a method of imaging non-periodic isolated objects at resolutions only limited, in principle, by the largest scattering angles recorded. We demonstrate X-ray diffraction imaging with high resolution…

Accurate dose distribution prediction is crucial in the radiotherapy planning. Although previous methods based on convolutional neural network have shown promising performance, they have the problem of over-smoothing, leading to prediction…

图像与视频处理 · 电气工程与系统科学 2024-10-31 Xin Liao , Zhenghao Feng , Jianghong Xiao , Xingchen Peng , Yan Wang

Astronomical imaging with micro-arcsecond ($\mu$as) angular resolution could enable breakthrough scientific discoveries. Previously-proposed $\mu$as X-ray imager designs have been interferometers with limited effective collecting area. Here…

天体物理仪器与方法 · 物理学 2020-06-24 Brandon D. Chalifoux , Ralf K. Heilmann , Herman L. Marshall , Mark L. Schattenburg

Quantum diamond microscopy (QDM), which employs nitrogen-vacancy (NV) center ensembles, is a promising approach to quantitatively imaging magnetic fields with both high resolution that approaches the diffraction limit and a wide field of…

光学 · 物理学 2025-05-20 Shunsuke Nishimura , Moeta Tsukamoto , Kento Sasaki , Kensuke Kobayashi

We present a theoretical evaluation of radiation dose constraints for extreme ultraviolet (EUV) and soft X-ray microscopy. Our work particularly addresses the long-standing concern regarding strong absorption of EUV radiation in biological…

应用物理 · 物理学 2026-05-29 Chang Liu , Leona Licht , Jan Rothhardt

Radiation damage is considered to be the major problem that still prevents imaging an individual biological molecule for structural analysis. So far, all known mapping techniques using sufficient short wave-length radiation, be it X-rays or…

生物物理 · 物理学 2010-10-28 Matthias Germann , Tatiana Latychevskaia , Conrad Escher , Hans-Werner Fink

Diffraction-before-destruction imaging with single ultrashort X-ray pulses has the potential to visualise non-equilibrium processes, such as chemical reactions, at the nanoscale with sub-femtosecond resolution in the native environment…

We demonstrate that powder diffraction data can be collected from sub-micron crystals of a mbrane protein with nearly two orders of magnitude more atoms than the molecules commonly used for powder diffraction. The crystals of photosystem-1…

The development of three-dimensional (3D) non-destructive X-ray characterization techniques in home laboratories is essential for enabling many more researchers to perform 3D characterization daily, overcoming the limitations imposed by…

High angular resolution X-ray imaging is always demanded by astrophysics and solar physics, which can be realized by coded-mask imaging with very long mask-detector distance in principle. Previously the diffraction-interference effect has…

天体物理学 · 物理学 2009-11-13 Chen Zhang , Shuang Nan Zhang

The X-ray free electron lasers (XFEL) can enable diffractive structural determination of protein crystals or single molecules that are too radiation-sensitive for conventional X-ray analysis. However the electronic form factor could have…

量子物理 · 物理学 2017-01-30 Zheng Li , Nikita Medvedev , Henry Chapman , Yanhua Shih

X-ray diffraction with high spatial resolution is a prerequisite for the characterization of (poly)-crystalline materials on micro- or nanoscopic scales. This can be achieved by utilizing a focused X-ray beam and scanning of the sample.…

材料科学 · 物理学 2023-01-04 Prerana Chakrabarti , Peter Modregger

Modern technology for producing extremely bright and coherent X-ray laser pulses provides the possibility to acquire a large number of diffraction patterns from individual biological nanoparticles, including proteins, viruses, and DNA.…

统计方法学 · 统计学 2018-07-11 Stefan Engblom , Carl Nettelblad , Jing Liu

Pencil x-ray beam imaging provides superior spatial resolution than other imaging geometries like sheet beam and cone beam geometries due to the illumination of a line instead of an area or volume. However, the pencil beam geometry suffers…

医学物理 · 物理学 2021-02-01 Ignacio O. Romero , Changqing Li
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