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In order to discern aggregation in solutions, we present a quantum mechanical analog of the photon statistics from fluorescent molecules diffusing through a focused beam. A generating functional is developed to fully describe the…

生物物理 · 物理学 2009-11-10 Hai-cang Ren , Noel L. Goddard , Gregoire Altan-Bonnet , Albert Libchaber

In this paper are reviewed various designs of advanced, multi-aperture optical systems dedicated to high angular resolution imaging or to the detection of exo-planets by nulling interferometry. A simple Fourier optics formalism applicable…

光学 · 物理学 2016-01-20 Francois Henault

Efficiently collecting light emitted or scattered from nanoscale systems, which can be embedded in a high-index medium, is a challenge for fundamental spectroscopic studies and commercial applications of quantum dots, color centers, single…

光学 · 物理学 2019-05-31 Jordi Puig , Christophe Galland

Diffraction is a manifestation of light at edge due to its wavelike nature. The well-known diffraction phenomena are Fresnel and Fraunhofer, they find variety of applications individually. But the synergy of two phenomena is not studied and…

物理教育 · 物理学 2020-12-15 Pritam P Shetty , Jayachandra Bingi

A fluorescence correlation spectroscopy (FCS) system based on two independent measurement volumes is presented. The optical setup and data acquisition hardware are detailed, as well as a complete protocol to control the location, size and…

光学 · 物理学 2009-08-26 P. Ferrand , M. Pianta , A. Kress , A. Aillaud , H. Rigneault , D. Marguet

A low cost fluorescence-based optical system is developed for detecting the presence of certain microorganisms and molecules within a diluted sample. A specifically designed device setup compatible with conventional 96 well plates is chosen…

计算机视觉与模式识别 · 计算机科学 2026-04-17 Zhendong Cao , Katrina G. Salvante , Ash Parameswaran , Pablo A. Nepomnaschy , Hongji Dai

Effective light extraction from optically active solid-state spin centres inside high-index semiconductor host crystals is an important factor in integrating these pseudo-atomic centres in wider quantum systems. Here we report increased…

This paper describes a micro fluorescence in situ hybridization ({\mu}FISH)-based rapid detection of cytogenetic biomarkers on formalin-fixed paraffin embedded (FFPE) tissue sections. We demonstrated this method in the context of detecting…

组织与器官 · 定量生物学 2018-06-19 Deborah Huber , Govind V. Kaigala

Fluorescence detection, either involving propagating or near-field emission, is widely being used in spectroscopy, sensing and microscopy. Total internal reflection fluorescence (TIRF) confines fluorescence excitation by an evanescent…

定量方法 · 定量生物学 2020-06-24 Martin Oheim , Adi Salomon , Maia Brunstein

We report on the fabrication and diffraction-limited characterization of parabolic focusing micromirrors. Sub - micron beam waists are measured for mirrors with 10 {\mu}m radius aperture and measured fixed focal lengths in the range from 24…

光学 · 物理学 2015-02-16 Alison McDonald , Gail McConnell , David C. Cox , Erling Riis , Paul F. Griffin

Microscale imaging of mesoscale bulk materials under dynamic compression is important for understanding their properties. In this work, we study the effects of the depth of field (DoF) and field of view (FoV) of the optical lens and extract…

Free-electron lasers (FEL) in the extreme ultraviolet (XUV) and X-ray regime opened up the possibility for experiments at high power densities, in particular allowing for fluence-dependent absorption and scattering experiments to reveal…

We propose and demonstrate an interferometric scheme for measuring the two-dimensional two-point cross-spectral density function in a two-shot manner. Our scheme comprises a Michelson interferometer with a converging lens in one of the arms…

仪器与探测器 · 物理学 2018-08-01 Abhinandan Bhattacharjee , Shaurya Aarav , Anand K. Jha

The use of individual multimode optical fibers in endoscopy applications has the potential to provide highly miniaturized and noninvasive probes for microscopy and optical micromanipulation. A few different strategies have been proposed…

We present an experimental method allowing to quantify slip at the wall in viscous polymer fluids, based on the observation of the evolution under simple shear flow of a photobleached pattern within a fluorescent labeled polymer melt. This…

We investigate - both experimentally and theoretically - the inelastic interaction between fast electrons and the electromagnetic field scattered by metallic apertures and nanostructures on dielectric membranes using photon induced…

Droplet microfluidics combined with optical detection has become a powerful approach for high-throughput single-cell assays, but these systems often face limited sensitivity and signal heterogeneity due to optical and geometrical…

流体动力学 · 物理学 2026-03-05 Kanimozhi Kumaresan , Thaipally Sujith , Anil Prabhakar , Ashis Kumar Sen

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

光学 · 物理学 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

We studied the ultrafast third-order optical nonlinearity in a single crystal of multiferroic bismuth ferrite (BiFeO3) in the near-infrared range of 0.5-1.0 eV, where the material is fundamentally transparent,at room temperature. With pump…

强关联电子 · 物理学 2015-09-02 Eiichi Matsubara , Takeshi Mochizuki , Masaya Nagai , Toshimitsu Ito , Masaaki Ashida

Two-dimensional molecular aggregate (2DMA), a thin sheet of strongly interacting dipole molecules self-assembled at close distance on an ordered lattice, is a fascinating fluorescent material. It is distinctively different from the single…

光学 · 物理学 2022-06-01 Qing Hu , Dafei Jin , Sang Hoon Nam , Jun Xiao , Yongmin Liu , Xiang Zhang , Nicholas X. Fang