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Two-dimensional gel electrophoresis has been instrumental in the development of proteomics. Although it is no longer the exclusive scheme used for proteomics, its unique features make it a still highly valuable tool, especially when…

基因组学 · 定量生物学 2012-06-15 Thierry Rabilloud

Two-dimensional electrophoresis of proteins has preceded, and accompanied, the birth of proteomics. Although it is no longer the only experimental scheme used in modern proteomics, it still has distinct features and advantages. The purpose…

基因组学 · 定量生物学 2011-09-15 Thierry Rabilloud , Cécile Lelong

Authors of biomedical publications use gel images to report experimental results such as protein-protein interactions or protein expressions under different conditions. Gel images offer a concise way to communicate such findings, not all of…

信息检索 · 计算机科学 2014-03-06 Tobias Kuhn , Mate Levente Nagy , ThaiBinh Luong , Michael Krauthammer

Authors of biomedical publications often use gel images to report experimental results such as protein-protein interactions or protein expressions under different conditions. Gel images offer a way to concisely communicate such findings,…

信息检索 · 计算机科学 2012-09-10 Tobias Kuhn , Michael Krauthammer

Two-dimensional electrophoresis has nurtured the birth of proteomics. It is however no longer the exclusive setup used in proteomics, with the development of shotgun proteomics techniques that appear more fancy and fashionable…

基因组学 · 定量生物学 2013-12-05 Thierry Rabilloud

Two-dimensional gel electrophoresis has been instrumental in the birth and developments of proteomics, although it is no longer the exclusive separation tool used in the field of proteomics. In this review, a historical perspective is made,…

基因组学 · 定量生物学 2010-08-17 Thierry Rabilloud , Mireille Chevallet , Sylvie Luche , Cécile Lelong

Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It -combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. For its use in…

基因组学 · 定量生物学 2012-06-15 Thierry Rabilloud

One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear proteins lies in their strong interaction with nuclear…

Gel electrophoresis, a widely used technique to separate DNA according to their size and weight, generates images that can be analyzed automatically. Manual or semiautomatic image processing presents a bottleneck for further development and…

计算机视觉与模式识别 · 计算机科学 2016-11-17 Naima Kaabouch , Richard R. Schultz , Barry Milavetz , Lata Balakrishnan

The use of an extra SDS separation in a different buffer system provide a technique for deconvoluting 2D gel spots made of several proteins (Colignon et al. Proteomics, 2013, 13, 2077-2082). This technique keeps the quantitative analysis of…

基因组学 · 定量生物学 2013-11-18 Thierry Rabilloud

Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry. These techniques, dealing with proteins…

The science of solving clinical problems by analyzing images generated in clinical practice is known as medical image analysis. The aim is to extract information in an effective and efficient manner for improved clinical diagnosis. The…

计算机视觉与模式识别 · 计算机科学 2019-05-22 Syed Muhammad Anwar , Muhammad Majid , Adnan Qayyum , Muhammad Awais , Majdi Alnowami , Muhammad Khurram Khan

Two-dimensional electrophoresis is still a very valuable tool in proteomics, due to its reproducibility and its ability to analyze complete proteins. However, due to its sensitivity to dynamic range issues, its most suitable use in the…

基因组学 · 定量生物学 2013-09-11 Thierry Rabilloud , Sarah Triboulet

The two-dimensional (2D) ultrasound imaging is widely used in several fields. In this study, the potential of two-dimensional ultrasound imaging technique based on machine learning is shown. A line transmitter was placed at one side and a…

医学物理 · 物理学 2020-04-21 Yoshiki Nagatani , Shigeaki Okumura , Shuqiong Wu , Tetsuya Matsuda

We present a computational method for real-time, patient-specific simulation of 2D ultrasound (US) images. The method uses a large number of tracked ultrasound images to learn a function that maps position and orientation of the transducer…

图像与视频处理 · 电气工程与系统科学 2020-05-12 Cesare Magnetti , Veronika Zimmer , Nooshin Ghavami , Emily Skelton , Jacqueline Matthew , Karen Lloyd , Jo Hajnal , Julia A. Schnabel , Alberto Gomez

The quality and ease of proteomics analysis depends on the performance of the analytical tools used, and thus of the performances of the protein separation tools used to deconvolute complex protein samples. Among protein samples, membrane…

基因组学 · 定量生物学 2008-12-31 Thierry Rabilloud , Mireille Chevallet , Sylvie Luche , Cécile Lelong

Membrane and nuclear proteins of poor solubility have been separated by high resolution two-dimensional (2-D) gel electrophoresis. Isoelectric focusing with immobilized pH gradients leads to severe quantitative losses of proteins in the…

基因组学 · 定量生物学 2007-05-23 T. Rabilloud , C. Adessi , A. Giraudel , J. Lunardi

Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally…

基因组学 · 定量生物学 2010-05-07 Thierry Rabilloud

In medical imaging analysis, deep learning has shown promising results. We frequently rely on volumetric data to segment medical images, necessitating the use of 3D architectures, which are commended for their capacity to capture interslice…

图像与视频处理 · 电气工程与系统科学 2023-05-18 Ikboljon Sobirov , Numan Saeed , Mohammad Yaqub

Novel imaging technologies raise many questions concerning the adaptation of computer-aided decision support systems. Classification models either need to be adapted or even newly trained from scratch to exploit the full potential of…

图像与视频处理 · 电气工程与系统科学 2020-04-28 Georg Wimmer , Michael Gadermayr , Andreas Vécsei , Andreas Uhl
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