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Although carbon nanotubes are potential candidates for DNA encapsulation and subsequent delivery of biological payloads to living cells, the thermodynamical spontaneity of DNA encapsulation under physiological conditions is still a matter…

生物物理 · 物理学 2016-05-05 Fernando J. A. L. Cruz , Juan J. de Pablo , José P. B. Mota

Microchip electrokinetic methods are capable of increasing the sensitivity of molecular assays by enriching and purifying target analytes. However, their use is currently limited to assays that can be performed under a high external…

定量方法 · 定量生物学 2019-01-01 Xander F. van Kooten , Moran Bercovici , Govind V. Kaigala

Bacteriophages, phages for short, are viruses of bacteria. The majority of phages contain a double-stranded DNA genome packaged in a capsid at a density of ~500 mg/ml. This high density requires substantial compression of the normal B form…

生物大分子 · 定量生物学 2010-12-13 Debabrata Panja , Ian J. Molineux

In vivo configurations of dsDNA of bacteriophage viruses in a capsid are known to form hexagonal chromonic liquid crystal phases. This article studies the liquid crystal ordering of viral dsDNA in an icosahedral capsid, combining the…

软凝聚态物质 · 物理学 2020-02-26 Shawn Walker , Javier Arsuaga , M. Carme Calderer , Lindsey Hiltner , Mariel Vazquez

The process of compaction of high molecular weight DNA T4 is investigated directly in a AFM liquid cell. The AFM-images of globules formed by DNA molecules in the result of compaction in water-alcohol environments at high izopropanol…

软凝聚态物质 · 物理学 2011-07-22 M. O. Gallyamov , O. A. Pyshkina , V. G. Sergeyev , I. V. Yaminsky

We characterize the equilibrium thermodynamics of a thick polymer confined in a spherical region of space. This is used to gain insight into the DNA packaging process. The experimental reference system for the present study is the recent…

统计力学 · 物理学 2007-05-23 Davide Marenduzzo , Cristian Micheletti

Many industrial soft materials often include oil-in-water (O/W) emulsions at the core of their formulations. By using tuneable interface stabilizing agents, such emulsions can self-assemble into complex structures. DNA has been used for…

The energetic and transport properties of a double-stranded DNA dodecamer encapsulated in hydrophobic carbon nanotubes are probed employing two limiting nanotube diameters, D=4 nm and D=3 nm, corresponding to (51,0) and (40,0) zig-zag…

生物物理 · 物理学 2016-05-06 Fernando J. A. L. Cruz , Juan J. de Pablo , José P. B. Mota

Our study aims at a better control and understanding of the transfer of a complex [DNA supercoiled plasmid - dodecyltrimethylammonium surfactant] layer from a liquid-vapour water interface onto a silicon surface without any additional…

化学物理 · 物理学 2008-01-18 Frédérique Battin-Leclerc , A. A. Konnov , J. L. Jaffrezo , M. Legrand

Recent in vitro experiments have shown that DNA ejection from bacteriophage can be partially stopped by surrounding osmotic pressure when ejected DNA is digested by DNase I on the course of ejection. We argue in this work by combination of…

生物物理 · 物理学 2008-12-18 Meerim Jeembaeva , Martin Castelnovo , Frida Larsson , Alex Evilevitch

The distance-resolved effective interaction potential between two parallel DNA molecules is calculated by computer simulations with explicit tetravalent counterions and monovalent salt. Adding counterions first yields an attractive minimum…

软凝聚态物质 · 物理学 2009-11-10 E. Allahyarov , H. Löwen , G. Gompper

Several controversial issues concerning the packing of linear DNA in bacteriophages and globules are discussed. Exact relations for the osmotic pressure, capsid pressure and loading force are derived in terms of the hole size inside phages…

软凝聚态物质 · 物理学 2007-05-23 Theo Odijk

A theory of condensation and resolubilization of a dilute DNA solution with growing concentration of multivalent cations, N is suggested. It is based on a new theory of screening of a macroion by multivalent cations, which shows that due to…

软凝聚态物质 · 物理学 2009-10-31 T. T. Nguyen , I. Rouzina , B. I. Shklovskii

We show experimentally that a cheap glass microcapillary can accumulate {\lambda}-phage DNA at its tip and deliver the DNA into the capillary using a combination of electro-osmotic flow, pressure-driven flow, and electrophoresis. We develop…

We discuss how a balance can be established between the force acting to eject DNA from viral capsids and the force resisting its entry into a colloidal suspension which mimics the host cell cytoplasm. The ejection force arises from the…

生物物理 · 物理学 2007-05-23 A. Evilevitch , M. Castelnovo , C. M. Knobler , W. M. Gelbart

The volume occupied by unconstrained bacterial DNA in physiological solutions exceeds 1000 times the volume of the cell. Still, it is confined to a well defined region of the cell called the nucleoid, which occupies only a fraction of the…

生物物理 · 物理学 2016-09-27 Marc Joyeux

With the rapid increase of available digital data, DNA storage is identified as a storage media with high density and capability of long-term preservation, especially for archival storage systems. However, the encoding density (i.e., how…

新兴技术 · 计算机科学 2021-08-26 Bingzhe Li , Li Ou , David Du

The effective DNA-DNA interaction force is calculated by computer simulations with explicit tetravalent counterions and monovalent salt. For overcharged DNA molecules, the interaction force shows a double-minimum structure. The positions…

生物大分子 · 定量生物学 2009-11-10 E. Allahyarov , G. Gompper , H. Löwen

A liquid crystal continuum modeling framework for icosahedra bacteriophage viruses is developed and tested. The main assumptions of the model are the chromonic columnar hexagonal structure of confined DNA, the high resistance to bending and…

软凝聚态物质 · 物理学 2017-12-05 Javier Arsuaga , Maria-Carme Calderer , Lindsey Hiltner , Mariel Vazquez

DNA-coated colloids can crystallize into a multitude of lattices, ranging from face-centered cubic to diamond and thereby contribute to our understanding of crystallization and open avenues to producing structures with useful photonic…

软凝聚态物质 · 物理学 2026-04-17 Pepijn G. Moerman , Huang Fang , Thomas E. Videbæk , W. Benjamin Rogers , Rebecca Schulman
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