相关论文: High-Contrast Interferometric Imaging of Single-Mo…
More than twenty years ago, scientists succeeded in pushing the limits of optical detection to single molecules using fluorescence. This breakthrough has revolutionized biophysical measurements, but restrictions in photophysics and labeling…
Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is…
The ability to detect and identify molecules at high sensitivity without the use of labels or capture agents is important for medical diagnostics, threat identification, environmental monitoring, and basic science. Microtoroid optical…
Interferometric-based microscopies stand as powerful label-free approaches for monitoring and characterising chemical reactions and heterogeneous nanoparticle systems in real time with single particle sensitivity. Nevertheless, coherent…
Monitoring the kinetics and conformational dynamics of single enzymes is crucial in order to better understand their biological functions as these motions and structural dynamics are usually unsynchronized among the molecules. Detecting the…
Sensors that are able to detect and track single unlabelled biomolecules are an important tool both to understand biomolecular dynamics and interactions at nanoscale, and for medical diagnostics operating at their ultimate detection limits.…
Single-molecule fluorescence techniques have revolutionized our ability to study proteins. However, the presence of a fluorescent label can alter the protein structure and/or modify its reaction with other species. To avoid the need for a…
Current optical detection schemes for single molecules require light absorption, either to produce fluorescence or direct absorption signals. This severely limits the range of molecules that can be detected, because most molecules are…
We employ heterodyne interferometry to investigate the effect of a single organic molecule on the phase of a propagating laser beam. We report on the first phase-contrast images of individual molecules and demonstrate a single-molecule…
Lensless illumination single-pixel imaging with a multicore fiber (MCF) is a computational imaging technique that enables potential endoscopic observations of biological samples at cellular scale. In this work, we show that this technique…
In this paper we compare the performance of multi and single-mode interferometry for the estimation of the phase of the complex visibility. We provide a theoretical description of the interferometric signal which enables to derive the phase…
Single-molecule approaches to biology offer a powerful new vision to elucidate the mechanisms that underpin the functioning of living cells. However, conventional optical single molecule spectroscopy techniques such as F\"orster…
Current single molecule methods either rely on fluorescence or lack chemical information. Here we report stimulated Raman photothermal encoded scattering (SRPSCAT) microscopy for quantitative bond-selective imaging of single-biomolecule…
Label-free single-molecule detection is essential for studying biomolecules in their native state, yet having materials with intrinsic molecular specificity to identify a broad range of molecules without complex functionalization remains…
Label-free biosensors enable the monitoring of biomolecular interactions in real-time, which is key to the analysis of the binding characteristics of biomolecules. While refractometric optical biosensors are sensitive and well-established,…
Single molecule detection provides detailed information about molecular structures and functions, but it generally requires the presence of a fluorescent marker which can interfere with the activity of the target molecule or complicate the…
We compare performances of ground-based single-mode and multimode (speckle) interferometers in the presence of partial Adaptive Optics correction of atmospheric turbulence. It is first shown that for compact sources (i.e. sources smaller…
Interferometry techniques are essential for extracting phase information from optical systems, enabling precise measurements of dispersion and highly sensitive detection of perturbations. While phase sensing offers enhanced sensitivity…
Optical detection of individual nanometer-sized analytes, virus particles, and protein molecules holds great promise for understanding and control of biological samples and healthcare applications. As fluorescent labels impose restrictions…
Lensless illumination single-pixel imaging with a multicore fiber (MCF) is a computational imaging technique that enables potential endoscopic observations of biological samples at cellular scale. In this work, we show that this technique…