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Immunoaffinity-based liquid biopsies of circulating tumor cells (CTCs) hold great promise for cancer management, but typically suffer from low throughput, relative complexity and post-processing limitations. Here we address these issues…

定量方法 · 定量生物学 2026-04-29 Jing Yan , Jie Wang , Robert Dallmann , Renquan Lu , Jérôme Charmet

A novel and fast semi-automatic method for segmentation, locating and counting blood cells in an image is proposed. In this method, thresholding is used to separate the nucleus from the other parts. We also use Hough transform for circles…

图像与视频处理 · 电气工程与系统科学 2020-04-21 Hamed Sadeghi , Shahram Shirani , David W. Capson

Investigation of cell structure is hardly imaginable without bright-field microscopy. Numerous modifications such as depth-wise scanning or videoenhancement make this method being state-of-the-art. This raises a question what maximal…

图像与视频处理 · 电气工程与系统科学 2020-10-29 Kirill Lonhus , Renata Rychtarikova , Ganna Platonova , Dalibor Stys

Flow cytometry is a high-throughput technology used to quantify multiple surface and intracellular markers at the level of a single cell. This enables to identify cell sub-types, and to determine their relative proportions. Improvements of…

Thrombosis is a common complication following the surgical implantation of blood contacting devices, and is strongly influenced by the phenomenon of near-wall enrichment of platelets. This paper describes a multi-constituent continuum…

流体动力学 · 物理学 2017-05-09 Wei-Tao Wu , Nadine Aubry , James F. Antaki , Mehrdad Massoudi

Islet microencapsulation is a promising solution to diabetes treatment, but its quality control based on manual microscopic inspection is extremely low-throughput, highly variable and laborious. This study presents a high-throughput…

仪器与探测器 · 物理学 2018-12-31 Yibo Zhang , Michael Alexander , Sam Yang , Yinxu Bian , Elliot Botvinick , Jonathan R. T. Lakey , Aydogan Ozcan

We combine confocal imaging, microfluidics and image analysis to record 3D-images of cells in flow. This enables us to recover the full 3D representation of several hundred living cells per minute. Whereas 3D confocal imaging has thus far…

流体动力学 · 物理学 2017-09-19 S. Quint , A. F. Christ , A. Guckenberger , S. Himbert , L. Kaestner , S. Gekle , C. Wagner

This work describes a new microfluidic device developed for rapid screening of solubility diagrams. In several parallel channels, hundreds of nanoliter-volume droplets of a given solution are first stored with a gradual variation in the…

软凝聚态物质 · 物理学 2007-05-23 P. Laval , N. Lisai , J. -B. Salmon , M. Joanicot

Large-scale microfluidic microsystems with complex three-dimensional (3D) configurations are highly in demand by both fundamental research and industrial application, holding the potentials for fostering a wide range of innovative…

应用物理 · 物理学 2020-01-13 Zijie Lin , Jian Xu , Yunpeng Song , Xiaolong Li , Peng Wang , Wei Chu , Zhenhua Wang , Ya Cheng

Flow cytometry is a widespread and powerful technique, whose resolution is determined by its capacity to accurately distinguish fluorescently positive populations from negative ones. However, most informative results are discarded while…

Analyzing the morphological attributes of blood vessels plays a critical role in the computer-aided diagnosis of many cardiovascular and ophthalmologic diseases. Although being extensively studied, segmentation of blood vessels,…

图像与视频处理 · 电气工程与系统科学 2020-10-12 Yicheng Wu , Chengwei Pan , Shuqi Wang , Ming Zhang , Yong Xia , Yizhou Yu

Automated segmentation in medical image analysis is a challenging task that requires a large amount of manually labeled data. However, most existing learning-based approaches usually suffer from limited manually annotated medical data,…

计算机视觉与模式识别 · 计算机科学 2022-03-22 Chenyu You , Yuan Zhou , Ruihan Zhao , Lawrence Staib , James S. Duncan

Cardiovascular disease and chronic kidney disease are major complications of diabetes, leading to high morbidity and mortality. Early detection of these conditions is critical, yet traditional diagnostic markers often lack sensitivity in…

其他定量生物学 · 定量生物学 2025-10-20 Syed Ibad Hasnain

We describe an approach for modeling fluid concentration profiles in grid-based microfluidic chips for fluid mixing. This approach provides an algorithm that predicts fluid concentrations at the chip outlets. Our algorithm significantly…

计算工程、金融与科学 · 计算机科学 2019-11-19 Huong Luu , Marek Chrobak

Coronary Microvascular Dysfunction (CMD) is characterized by impaired vasodilation and can lead to insufficient blood flow to the myocardium during stress or exertion, affecting millions of people globally. Despite their diagnostic value,…

计算工程、金融与科学 · 计算机科学 2025-08-04 Haizhou Yang , Jiyang Zhang , Ismael Z. Assi , Brahmajee K. Nallamothu , Krishna Garikipati , C. Alberto Figueroa

The movement of molecules inside living cells is a fundamental feature of biological processes. The ability to both observe and analyse the details of molecular diffusion in vivo at the single molecule and single cell level can add…

定量方法 · 定量生物学 2012-11-21 Alex Robson , Kevin Burrage , Mark Leake

Accurate classification of haematological cells is critical for diagnosing blood disorders, but presents significant challenges for machine automation owing to the complexity of cell morphology, heterogeneities of biological, pathological,…

This chapter presents an overview of microfluidic devices reported in the literature, used to develop methodologies for nucleation of biomolecules, with crystal size control, and for collecting thermodynamic and kinetic data. Part I is…

软凝聚态物质 · 物理学 2025-12-16 Nadine Candoni , Romain Grossier , Stéphane Veesler

Multiple Sclerosis (MS) is a disorder that usually appears in adults in their thirties. It has a prevalence that ranges between 2 and 150 per 100 000. Epidemiological studies of MS have provided hints on possible causes for the disease…

亚细胞过程 · 定量生物学 2013-08-23 Laura Facchini , Alberto Bellin , Eleuterio F. Toro

Blood, a ubiquitous and fundamental carbohydrate material composed of plasma, red blood cells, white blood cells, and platelets, has been playing an important role in biology, life science, history, and religious study, while graphene has…

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