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We combine confocal imaging, microfluidics and image analysis to record 3D-images of cells in flow. This enables us to recover the full 3D representation of several hundred living cells per minute. Whereas 3D confocal imaging has thus far…

流体动力学 · 物理学 2017-09-19 S. Quint , A. F. Christ , A. Guckenberger , S. Himbert , L. Kaestner , S. Gekle , C. Wagner

Laser Doppler holography (LDH) is an interferometric blood flow imaging technique based on full-field measurements of the Doppler spectrum. LDH has so far been demonstrated in the retina with ultrafast cameras, typically at 75 kHz. We show…

图像与视频处理 · 电气工程与系统科学 2020-08-26 Leo Puyo , Michel Paques , Michael Atlan

We demonstrate a motion-free intensity diffraction tomography technique that enables direct inversion of 3D phase and absorption from intensity-only measurements for weakly scattering samples. We derive a novel linear forward model,…

图像与视频处理 · 电气工程与系统科学 2018-06-26 Ruilong Ling , Waleed Tahir , Hsing-Ying Lin , Hakho Lee , Lei Tian

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

Holotomography, a three-dimensional quantitative phase imaging technique, presents an innovative, non-invasive approach to studying biological samples by exploiting the refractive index as an intrinsic imaging contrast. Despite offering…

光学 · 物理学 2023-08-04 Herve Hugonnet , HyunJun Han , Weisun Park , YongKeun Park

Increasing demand for understanding the vast heterogeneity of cellular phenotypes has driven the development of imaging flow cytometry (IFC), that combines features of flow cytometry with fluorescence and bright field microscopy. IFC…

定量方法 · 定量生物学 2019-02-06 Yuanyuan Han , Rui Tang , Yi Gu , Alex Ce Zhang , Wei Cai , Violet Castor , Sung Hwan Cho , William Alaynick , Yu-Hwa Lo

The measurement of three-dimensional (3D) images and the analysis of subcellular organelles are crucial for the study of the pathophysiology of cells and tissues. Optical diffraction tomography (ODT) facilitates label-free and quantitative…

图像与视频处理 · 电气工程与系统科学 2019-10-08 Taean Chang , Seungwoo Shin , Moosung Lee , YongKeun Park

DeepAngle is a machine learning-based method to determine the contact angles of different phases in the tomography images of porous materials. Measurement of angles in 3--D needs to be done within the surface perpendicular to the angle…

计算机视觉与模式识别 · 计算机科学 2022-11-29 Arash Rabbani , Chenhao Sun , Masoud Babaei , Vahid J. Niasar , Ryan T. Armstrong , Peyman Mostaghimi

Circulating tumor cells (CTCs) are cancer cells found in the bloodstream that serve as biomarkers for early cancer detection, prognostication, and disease monitoring. However, CTC detection remains challenging due to low cell abundance and…

Optical diffraction tomography (ODT) is a three-dimensional (3D) label-free imaging technique. The 3D refractive index distribution of a sample can be reconstructed from multiple two-dimensional optical field images via ODT. Herein, we…

光学 · 物理学 2021-02-03 Chansuk Park , Kyeoreh Lee , Yoonseok Baek , Yongkeun Park

Accelerating imaging speed in optical microscopy is often realized at the expense of image contrast, image resolution, and detection sensitivity- a common predicament for advancing high-speed and high-throughput cellular imaging. We here…

Correlation filters are special classifiers designed for shift-invariant object recognition, which are robust to pattern distortions. The recent literature shows that combining a set of sub-filters trained based on a single or a small group…

计算机视觉与模式识别 · 计算机科学 2018-02-14 Baochang Zhang , Shangzhen Luan , Chen Chen , Jungong Han , Wei Wang , Alessandro Perina , Ling Shao

We propose a new model-free method to detect change points between distinct phases in a single random trajectory of an intermittent stochastic process. The local convex hull (LCH) is constructed for each trajectory point, while its…

统计力学 · 物理学 2019-11-05 Y. Lanoiselée , D. S. Grebenkov

We present a lensless, interferenceless incoherent digital holography technique based on the principle of coded aperture correlation holography. The acquired digital hologram by this technique contains a three-dimensional image of some…

光学 · 物理学 2017-08-02 Manoj Kumar , A. Vijayakumar , Joseph Rosen

Laser Doppler holography (LDH) is a full-field imaging technique that was recently used in the human eye to reveal blood flow contrasts in the retinal and choroidal vasculature non-invasively, and with high temporal resolution. We here…

医学物理 · 物理学 2020-04-01 L. Puyo , M. Paques , M. Fink , J-A Sahel , M. Atlan

According to time-distance helioseismology, information about internal fluid motions is encoded in the travel times of solar waves. The inverse problem consists of inferring 3-D vector flows from a set of travel-time measurements. Here we…

太阳与恒星天体物理 · 物理学 2015-05-27 M. Svanda , L. Gizon , S. M. Hanasoge , S. D. Ustyugov

Cells alter the path of light, a fact that leads to well-known aberrations in single cell or tissue imaging. Optical diffraction tomography (ODT) measures the biophysical property that causes these aberrations, the refractive index (RI).…

定量方法 · 定量生物学 2017-08-14 Mirjam Schürmann , Gheorghe Cojoc , Salvatore Girardo , Elke Ulbricht , Jochen Guck , Paul Müller

The ability to resolve and quantify features at submicrometer scales from a single-shot image is crucial for real-time uncovering intricate structures in unlabeled biological samples and analyzing them at the subcellular level. We introduce…

光学 · 物理学 2025-01-22 Jaromír Běhal , Miroslav Ježek

Recent innovations shows that blending of details captured by single Low Dynamic Range (LDR) sensor overcomes the limitations of standard digital cameras to capture details from high dynamic range scene. We present a method to produce…

计算机视觉与模式识别 · 计算机科学 2022-07-12 Harbinder Singh , Dinesh Arora , Vinay Kumar

Internal properties of a sample can be observed by medical imaging tools, such as ultrasound devices, magnetic resonance imaging (MRI) and optical coherence tomography (OCT) which are based on relying on changes in material density or…

计算机视觉与模式识别 · 计算机科学 2020-12-22 Mehrdad Shafiei Dizaji , Devin Harris
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