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Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

光学 · 物理学 2022-01-26 Yi Xue , David Ren , Laura Waller

We report a computational 3D microscopy technique, termed Fourier ptychographic diffraction tomography (FPDT), that iteratively stitches together numerous variably illuminated, low-resolution images acquired with a low-numerical aperture…

光学 · 物理学 2020-02-19 Chao Zuo , Jiasong Sun , Jiaji Li , Anand Asundi , Qian Chen

Intensity diffraction tomography (IDT) refers to a class of optical microscopy techniques for imaging the 3D refractive index (RI) distribution of a sample from a set of 2D intensity-only measurements. The reconstruction of artifact-free RI…

图像与视频处理 · 电气工程与系统科学 2022-08-16 Renhao Liu , Yu Sun , Jiabei Zhu , Lei Tian , Ulugbek Kamilov

We present a tomographic imaging technique, termed Deep Prior Diffraction Tomography (DP-DT), to reconstruct the 3D refractive index (RI) of thick biological samples at high resolution from a sequence of low-resolution images collected…

图像与视频处理 · 电气工程与系统科学 2021-11-18 Kevin C. Zhou , Roarke Horstmeyer

Optical diffraction tomography (ODT) has emerged as a powerful label-free three-dimensional (3D) bioimaging techniques for observing living cells and thin tissue layers. We report a new reflection-mode ODT (rODT) method for imaging thick…

Optical diffraction tomography (ODT) reconstructs a samples volumetric refractive index (RI) to create high-contrast, quantitative 3D visualizations of biological samples. However, standard implementations of ODT use interferometric…

图像与视频处理 · 电气工程与系统科学 2024-12-13 Shwetadwip Chowdhury , Michael Chen , Regina Eckert , David Ren , Fan Wu , Nicole Repina , Laura Waller

This work introduces a novel reinterpretation of structured illumination (SI) microscopy for coherent imaging that allows three-dimensional imaging of complex refractive index (RI). To do so, we show that coherent SI is mathematically…

光学 · 物理学 2017-05-11 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

Optical diffraction tomography (ODT) provides three-dimensional refractive index (RI) tomograms of a transparent microscopic object. However, because of the finite numerical aperture of objective lenses, ODT has the limited access to…

光学 · 物理学 2017-11-22 Moosung Lee , Seungwoo Shin , YongKeun Park

Optical diffraction tomography (ODT) is an interferometric microscopy technique capable of measuring 3-D refractive index (RI) distribution of transparent samples. Multiple 2-D holograms of a sample illuminated with various angles are…

Optical diffraction tomography (ODT) is a three-dimensional (3D) label-free imaging technique. The 3D refractive index distribution of a sample can be reconstructed from multiple two-dimensional optical field images via ODT. Herein, we…

光学 · 物理学 2021-02-03 Chansuk Park , Kyeoreh Lee , Yoonseok Baek , Yongkeun Park

The measurement of three-dimensional (3D) images and the analysis of subcellular organelles are crucial for the study of the pathophysiology of cells and tissues. Optical diffraction tomography (ODT) facilitates label-free and quantitative…

图像与视频处理 · 电气工程与系统科学 2019-10-08 Taean Chang , Seungwoo Shin , Moosung Lee , YongKeun Park

Optical diffraction tomography (ODT) enables non-invasive information-rich 3D refractive index (RI) reconstruction of unimpaired transparent biological and technical samples, crucial in biomedical research, optical metrology, materials…

Under-display ToF imaging aims to achieve accurate depth sensing through a ToF camera placed beneath a screen panel. However, transparent OLED (TOLED) layers introduce severe degradations-such as signal attenuation, multi-path interference…

计算机视觉与模式识别 · 计算机科学 2025-11-04 Xin Qiao , Matteo Poggi , Xing Wei , Pengchao Deng , Yanhui Zhou , Stefano Mattoccia

Diffraction tomography (DT) has been widely explored in transmission-mode configurations, enabling high-resolution, label-free 3D imaging. However, industrial metrology applications, such as semiconductor inspection, typically involve…

光学 · 物理学 2026-02-04 Jiabei Zhu , Tongyu Li , Hao Wang , Yi Shen , Guorong Hu , Lei Tian

Cells alter the path of light, a fact that leads to well-known aberrations in single cell or tissue imaging. Optical diffraction tomography (ODT) measures the biophysical property that causes these aberrations, the refractive index (RI).…

定量方法 · 定量生物学 2017-08-14 Mirjam Schürmann , Gheorghe Cojoc , Salvatore Girardo , Elke Ulbricht , Jochen Guck , Paul Müller

Non-contact measurement of the refractive index and thickness of multilayer biological tissues is of great significance for biomedical applications and can greatly improve medical diagnosis and treatment. In this work, we introduce a…

光学 · 物理学 2024-04-19 Yu-Lin Ku , Yao-Gen Shu

Label-free tomographic microscopy offers a compelling means to visualize three-dimensional (3D) refractive index (RI) distributions from two-dimensional (2D) intensity measurements. However, limited forward-model accuracy and the ill-posed…

光学 · 物理学 2025-02-27 Delong Yang , Shaohui Zhang , Jiasong Sun , Chao Zuo , Qun Hao

We present an alternative numerical reconstruction algorithm for direct tomographic reconstruction of a sample refractive indices from the measured intensities of its far-field coherent diffraction patterns. We formulate the well-known…

计算物理 · 物理学 2019-02-06 T. Ramos , Bastian E. Grønager , Martin Skovgaard Andersen , J. W. Andreasen

Coherent diffractive imaging (CDI) provides new opportunities for high resolution X-ray imaging with simultaneous amplitude and phase contrast. Extensions to CDI broaden the scope of the technique for use in a wide variety of experimental…

光学 · 物理学 2016-01-20 Matthew H. Seaberg , Alexandre d'Aspremont , Joshua J. Turner

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

光学 · 物理学 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels
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