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Immunofluorescent (IF) imaging is crucial for visualizing biomarker expressions, cell morphology and assessing the effects of drug treatments on sub-cellular components. IF imaging needs extra staining process and often requiring cell…

Quantitative analysis of cell structures is essential for biomedical and pharmaceutical research. The standard imaging approach relies on fluorescence microscopy, where cell structures of interest are labeled by chemical staining…

Staining is critical to cell imaging and medical diagnosis, which is expensive, time-consuming, labor-intensive, and causes irreversible changes to cell tissues. Recent advances in deep learning enabled digital staining via supervised model…

图像与视频处理 · 电气工程与系统科学 2023-03-06 Ziwang Xu , Lanqing Guo , Shuyan Zhang , Alex C. Kot , Bihan Wen

With the rapid development of deep generative models (such as Generative Adversarial Networks and Diffusion models), AI-synthesized images are now of such high quality that humans can hardly distinguish them from pristine ones. Although…

计算机视觉与模式识别 · 计算机科学 2023-09-06 Yan Ju , Shan Jia , Jialing Cai , Haiying Guan , Siwei Lyu

Multiplex immunofluorescence (MxIF) is an emerging imaging technique that produces the high sensitivity and specificity of single-cell mapping. With a tenet of 'seeing is believing', MxIF enables iterative staining and imaging extensive…

图像与视频处理 · 电气工程与系统科学 2021-09-21 Shunxing Bao , Yucheng Tang , Ho Hin Lee , Riqiang Gao , Sophie Chiron , Ilwoo Lyu , Lori A. Coburn , Keith T. Wilson , Joseph T. Roland , Bennett A. Landman , Yuankai Huo

Multiplex brightfield imaging offers the advantage of simultaneously analyzing multiple biomarkers on a single slide, as opposed to single biomarker labeling on multiple consecutive slides. To accurately analyze multiple biomarkers…

图像与视频处理 · 电气工程与系统科学 2024-08-16 Satarupa Mukherjee , Jim Martin , Yao Nie

Over the past years, image generation and manipulation have achieved remarkable progress due to the rapid development of generative AI based on deep learning. Recent studies have devoted significant efforts to address the problem of face…

计算机视觉与模式识别 · 计算机科学 2024-02-15 Yuhang Lu , Touradj Ebrahimi

The creation of in-silico datasets can expand the utility of existing annotations to new domains with different staining patterns in computational pathology. As such, it has the potential to significantly lower the cost associated with…

图像与视频处理 · 电气工程与系统科学 2024-03-12 Dominik Winter , Nicolas Triltsch , Philipp Plewa , Marco Rosati , Thomas Padel , Ross Hill , Markus Schick , Nicolas Brieu

In this paper, we propose a novel application of Generative Adversarial Networks (GAN) to the synthesis of cells imaged by fluorescence microscopy. Compared to natural images, cells tend to have a simpler and more geometric global structure…

计算机视觉与模式识别 · 计算机科学 2017-09-13 Anton Osokin , Anatole Chessel , Rafael E. Carazo Salas , Federico Vaggi

Generative artificial intelligence (AI) is rapidly transforming medical imaging by enabling capabilities such as data synthesis, image enhancement, modality translation, and spatiotemporal modeling. This review presents a comprehensive and…

图像与视频处理 · 电气工程与系统科学 2025-08-14 Xuanru Zhou , Cheng Li , Shuqiang Wang , Ye Li , Tao Tan , Hairong Zheng , Shanshan Wang

Multiple stains are usually used to highlight biological substances in biomedical image analysis. To decompose multiple stains for co-localization quantification, blind source separation is usually performed. Prior model-based stain…

图像与视频处理 · 电气工程与系统科学 2021-02-16 Xingyu Li

The automated analysis of medical images is currently limited by technical and biological noise and bias. The same source tissue can be represented by vastly different images if the image acquisition or processing protocols vary. For an…

Generative Artificial Intelligence (GenAI) is a field of AI that creates new data samples from existing ones. It utilizing deep learning to overcome the scarcity and regulatory constraints of healthcare data by generating new data points…

机器学习 · 计算机科学 2024-10-02 Sarthak Pati , Szymon Mazurek , Spyridon Bakas

Despite the recent success in applying supervised deep learning to medical imaging tasks, the problem of obtaining large and diverse expert-annotated datasets required for the development of high performant models remains particularly…

计算机视觉与模式识别 · 计算机科学 2019-11-21 Amirata Ghorbani , Vivek Natarajan , David Coz , Yuan Liu

Carrying out clinical diagnosis of retinal vascular degeneration using Fluorescein Angiography (FA) is a time consuming process and can pose significant adverse effects on the patient. Angiography requires insertion of a dye that may cause…

图像与视频处理 · 电气工程与系统科学 2021-10-20 Sharif Amit Kamran , Khondker Fariha Hossain , Alireza Tavakkoli , Stewart Lee Zuckerbrod , Salah A. Baker , Kenton M. Sanders

Many medical and biological protocols for analyzing individual biological cells involve morphological evaluation based on cell staining, designed to enhance imaging contrast and enable clinicians and biologists to differentiate between…

While hundreds of artificial intelligence (AI) algorithms are now approved or cleared by the US Food and Drugs Administration (FDA), many studies have shown inconsistent generalization or latent bias, particularly for underrepresented…

Recent advances in deep learning and on-device inference could transform routine screening for skin cancers. Along with the anticipated benefits of this technology, potential dangers arise from unforeseen and inherent biases. A significant…

计算机视觉与模式识别 · 计算机科学 2025-12-23 Ko Watanabe , Stanislav Frolov , Aya Hassan , David Dembinsky , Adriano Lucieri , Andreas Dengel

Simulating in silico cellular responses to interventions is a promising direction to accelerate high-content image-based assays, critical for advancing drug discovery and gene editing. To support this, we introduce MorphGen, a…

定量方法 · 定量生物学 2025-10-13 Berker Demirel , Marco Fumero , Theofanis Karaletsos , Francesco Locatello

Generation of photo-realistic images, semantic editing and representation learning are a few of many potential applications of high resolution generative models. Recent progress in GANs have established them as an excellent choice for such…

计算机视觉与模式识别 · 计算机科学 2021-12-13 Partha Ghosh , Dominik Zietlow , Michael J. Black , Larry S. Davis , Xiaochen Hu
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